LOCUS       sequence-332371-        8531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid pWSK29 encoding E. coli LacZ' for making reporter fusion 
            proteins for topology mapping.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Daniels C, Vindurampulle C, Morona R
  TITLE     Overexpression and topology of the Shigella flexneri O-antigen 
            polymerase (Rfc/Wzy).
  JOURNAL   Mol Microbiol. 1998 Jun;28(6):1211-22. doi: 
            10.1046/j.1365-2958.1998.00884.x.
  PUBMED    9680210
REFERENCE   2  (bases 1 to 8531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1046/j.1365-2958.1998.00884"; journalName: "Mol Microbiol"; 
            date: "1998-06"; volume: "28"; issue: "6"; pages: "1211-22"
COMMENT     Sequence Label: pRMCD70
FEATURES             Location/Qualifiers
     source          1..8531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1463..1482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1464..1482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1492..1509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1492..1508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1650..2105
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1737..1756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1947..1968
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2131..2235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2236..2304,2305..3096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2454..2473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4117..5067)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5115..5337
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     primer_bind     6482..6499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6616..6637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6652..6657,6658..6675,6676..6682)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6690..6706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6695..6717
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6714..6730
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6714..6730
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6749..6769
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6751..6769
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     6806..6822
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6806..6822
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(6853..8531,1..1369)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
ORIGIN
        1 ggactgggtg gatcagtcgc tgattaaata tgatgaaaac ggcaacccgt ggtcggctta
       61 cggcggtgat tttggcgata cgccgaacga tcgccagttc tgtatgaacg gtctggtctt
      121 tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa caccagcagc agtttttcca
      181 gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga
      241 taacgagctc ctgcactgga tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt
      301 gcctctggat gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc
      361 ggagagcgcc gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg
      421 gtcagaagcc gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt
      481 gacgctcccc gccgcgtccc acgccatccc gcatctgacc accagcgaaa tggatttttg
      541 catcgagctg ggtaataagc gttggcaatt taaccgccag tcaggctttc tttcacagat
      601 gtggattggc gataaaaaac aactgctgac gccgctgcgc gatcagttca cccgtgcacc
      661 gctggataac gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga
      721 acgctggaag gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga
      781 tacacttgct gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac
      841 cttatttatc agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt
      901 tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct
      961 ggcgcaggta gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga
     1021 ccgccttact gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc
     1081 gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc
     1141 acaccagtgg cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat
     1201 ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc acatggctga atatcgacgg
     1261 tttccatatg gggattggtg gcgacgactc ctggagcccg tcagtatcgg cggaattaca
     1321 gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg
     1381 caggccatgt ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt tgacctcgag
     1441 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     1501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1621 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     1681 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1741 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1801 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1861 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     1921 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1981 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2041 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2101 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2221 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2281 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2341 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2401 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2461 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2521 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2581 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2641 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2701 catgtaactc gccttgatcg ctgggaaccg gagctgaatg aagccatacc aaacgacgag
     2761 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     2821 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     2881 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     2941 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3001 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3061 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3121 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3181 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagat
     3241 ctctgttcgg gggttcactg acgctgcgtg actacgatgg tcaggttgcg gaggctatgg
     3301 ccctggtacg agcgctgaac aaaatgacga aagcaggtat gcctgaaagc gtgcgtattg
     3361 cctgaaaaca caacccgcta cgggggagac ttacccgaaa tctgatttat tcaacaaagc
     3421 cactttcagt atggatttgg gtgttacggg ggggggttgg tgcttaaaat cgcgctgagg
     3481 atttctgatg gtgttaagcg ggcggttttg agatgtaaac tcgcccgttt aacataatgg
     3541 atcttgcgcg caccgcccga acaccactcg ccacaaaaaa ccgccggaac gtccaaaagt
     3601 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
     3661 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
     3721 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
     3781 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
     3841 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
     3901 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
     3961 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     4021 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     4081 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     4141 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     4201 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     4261 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     4321 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     4381 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     4441 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     4501 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     4561 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     4621 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     4681 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     4741 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     4801 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     4861 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     4921 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     4981 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     5041 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     5101 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     5161 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     5221 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     5281 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     5341 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     5401 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     5461 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     5521 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     5581 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     5641 ggcgttttat ggcgggtctg cggagtggtg aatccgttag cgaggtgccg ccggcttcca
     5701 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     5761 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     5821 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc
     5881 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
     5941 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
     6001 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
     6061 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
     6121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
     6181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
     6241 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     6301 ttgaaggctc tcaagggcat cggcattcgc cattcaggct gcctcctacc aagacgactt
     6361 caaatccgac ccgtagagat ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     6421 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     6481 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     6541 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     6601 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     6661 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     6721 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     6781 ggagctccac cgcggtggcg gccgctctag aactagtgga tcccccgggc tgcaggaatt
     6841 cccggggatc ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6901 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6961 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     7021 gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     7081 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc
     7141 tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     7201 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     7261 ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
     7321 agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
     7381 gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg
     7441 atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca aatcagcgat
     7501 ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga ggctgaagtt
     7561 cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg gcagggtgaa
     7621 acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt
     7681 tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa
     7741 atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
     7801 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
     7861 aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt
     7921 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
     7981 aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
     8041 tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
     8101 cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg
     8161 cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
     8221 ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg
     8281 cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
     8341 cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt
     8401 tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac
     8461 agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg tttacagggc
     8521 ggcttcgtct g
//
