LOCUS       sequence-332389-        8765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BeCyclOp in BWMs of C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Gao S, Nagpal J, Schneider MW, Kozjak-Pavlovic V, Nagel G, 
            Gottschalk A
  TITLE     Optogenetic manipulation of cGMP in cells and animals by the tightly
            light-regulated guanylyl-cyclase opsin CyclOp.
  JOURNAL   Nat Commun. 2015 Sep 8;6:8046. doi: 10.1038/ncomms9046.
  PUBMED    26345128
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-09-8"; volume: "6:8046. doi"; pages: " 
            10.1038/ncomms9046"
COMMENT     Sequence Label: pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    83..104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(119..124,125..142,143..149)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    157..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     162..184
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     181..197
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     181..197
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4901..5611
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5046..5064)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5314..5333
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(6527..6549)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6649..6668
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6750..6768)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6836..6940
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6941..7009,7010..7801)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7159..7178)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7972..8560
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8461..8480
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8714..8731
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
       61 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      121 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      181 caggaaacag ctatgaccat gattacgcca agcttgcggc tataataagt tcttgaataa
      241 aataattttc ccgacaaaac atgagtattt ctttcgaaaa taaaagtgca ggctaattag
      301 agattattct gtaattaact gcataatttg tcacgtgcca tagttttaca ttccactacg
      361 tcatagttct taaaatacta atctcctgaa aatagaagta ggtgaagaaa gtttaattat
      421 cagttctaaa atgacaattg atctttggaa tatgttctga aactaccgat cattgaacag
      481 atgctatttg aatgatatag aattgtatat ttgcaatttc tgaaacgcgt tcttaaaggc
      541 acacagatta attcaaaagg gtctggccgc aaaaaggttt atggtggccg attttgagtt
      601 ttgtgtgtga ttgctttttc acaatcagtg ttttcaggat tatgtgatga actagatctt
      661 caagtttcgt tacatttcat atgttttcgg aactcacgaa gtacatattg ggtattgtgc
      721 tcaaaaaatt cagcaatcag cttcgctccg ctgactttag aacccaaaaa aatagtatgg
      781 ccaaactgac tgtgttacga tcatttcaat ttttcaatac atatttaaga tttctaagag
      841 taagaaggtc aaaaactgtt ctggaataca tatatatttt tcaggttaca attagtcaaa
      901 aagtgcactg aaatatacgt tttaatttca cgaataaccc aattagttca atgtattttt
      961 ggtcaaccaa cgttaaagtt tggcttccaa ccaattatca tttctgatca accacaatgt
     1021 tttttcttta tctgcaagtt aattttttat ttttatccag atgtttggca tatttttcaa
     1081 ttcttcacta gcgcccactt cttgcacttc cggcgccctg aatctaatgc atctgttgca
     1141 agaattgaaa gaccaatcaa cacattgttt tcttcacgag atactgaaga aaatgaataa
     1201 aaacagagaa aaagagccat gtgattagtg acaactgttg ctaacagata ccatagcttg
     1261 gacttggtac gtgatggcaa cgtatgggtc aacaaaaatg attgcagagg gggtgcaaaa
     1321 cagtcaagtc gagaaaatat gaaaaacaga aaacaaagaa cagaaaaatg ggtttgagag
     1381 tcagtataat ttataaaaga aaaattgtac atagaaatta accatttttg tagaagaagt
     1441 tatttttcaa gcatcgttaa aaattattca aagcacctta tttcatattt aattttaaac
     1501 atggttaaat gaacaacacg gtgcgcaatc aggaaaactt gaaatctgaa actgttgttg
     1561 tgatcttctt cgcaactgtt cagatagcac tagtgtaatg ttaagagtgc gcgaatataa
     1621 tggaatataa tggatcacac ctcctgccat caggtaaacg tctctgttat cacatatttc
     1681 caactattaa atttttacct tttacagttt tacatttttt tgaaaaaagt aactttttgt
     1741 cttcaaaatc cctgacgaaa atatcaaata ttttaatcga gactgcagag gaaccgattg
     1801 atgatttgga aaatccagct ttacctgtgt aagaactgaa aagtttcata accctagggt
     1861 attcccagtt acattcccca ctggctaaca atagcaccca gtttttcatc acctttcttc
     1921 aaatttctcg gcgatttgtt aaaaacaaaa tttgtgtccc ttctctgata tctctatgtc
     1981 tctaaacaca agttcatcgg aaaacgaagg agggtaggtg ttggttgggc tcccgaagtg
     2041 aaaatagaag agcaagaata gaatattaga gagagagtgc agagagggcg ggatagctcc
     2101 cgggattccg ttttcttctt ctttatcttc aacgatgatg tgtgtgcgtg ttgtatagat
     2161 tctgttgctc ccccacaact cgctccgaag gctcaataca attcaattga tattggagga
     2221 gagcctaccg gagtgggagg ataagaagaa acataagaag aagaagaaga agaagcatgc
     2281 ttctggtttt tgatgctatg aaaacggcac aaaaagatga ttgaggtccc ttttcaatac
     2341 cttctctcat ctttcaaatc ccattgaaac ctaaaacttc tcaccacgct ttaccattgt
     2401 tctccaaaaa cttatagcaa tgtctataac ttttttatct ctgaaaagca gtgttccatt
     2461 tttctttttc ctattttatt tcaattgttt ctcacatttc gtttggattc tttgcttgtc
     2521 aaccagcttc ttcttccact tttaccgtct aattttcagg gcagggagcc atcaaaccca
     2581 cgaccactag atccatctag aatgaaggac aaggacaaca acctccgtgg agcctgctcc
     2641 tcctgcaact gcccagagta ctgcttctcc ccaacctcca ccctctgcga cgactgcaag
     2701 tgctccgtca ccaagcaccc aatcgtcgag caaccactct cccgtaacgg atccttccgt
     2761 tcctccggag cctccctcct cccatcccca tcctccccaa acgtcaagat cacctccacc
     2821 gtcggactcc gttcccgtaa gtccgagtcc caagccaacg tccgtggatc catgatctcc
     2881 aactccaact ccggatcccg ttccaacaac tccggaggag ccggaggagg atccggagga
     2941 tcctcctcct ccaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     3001 tttcagggag gatccgccct cgccaactac caatccgcca tgtccgagct ctggtcctgg
     3061 aacatgatgc tctccacccc atccctcaag ttcctcaccg tccaattcac cacctggatc
     3121 gtcctcacca ccgtcggagc catctacacc ctcttcttcc acgagcgcca agcctacaac
     3181 cgtggatggg ccgacatctg gtacggatac ggagccttcg gattcggact cggactctcc
     3241 ttcgcctaca tgggattcac cggagcccgt aacccagaga agaaggccct ctccctctgc
     3301 ctcctcggag tcaacttcat ctccttcatg tcctacatca tcatcatgct ccgtctcacc
     3361 ccaaccatcg agggaaccat ggccaaccca gtcgagccag cccgttacct cgagtggatc
     3421 gccacctgcc cagtcctcat cctcctcatc tccgagatca cccaataccc acacgaccca
     3481 tacaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtc
     3541 atcgtcaacg actacgccct ctgcctcgcc ggattcgtcg gagccatctc cgcccaacaa
     3601 ccatggggag acctcgccca cttcgtctcc tgcctctgct tctcctacgt cgtctactcc
     3661 ctctggtcct gcttcaccgg agccatcgac ggagagaccc aatgcaacgt cgagaagtcc
     3721 ggactccgtt ggatccgttt ctccaccatc accacctggt ccctcttccc aatcacctgg
     3781 ttctcctaca cctccggact catctccttc accgtcgccg aggccggatt ctccatgatc
     3841 gacatcggag ccaaggtctt cctcaccctc gtcctcgtca actccaccgt cgagcaagcc
     3901 caaaaccaaa aggtcgacgc catcaccgcc atcgccgagg agctcgagaa ccaaatcaac
     3961 aactgcgacg ccatcctcca aaagatgatg ccagagggag tcctcgagca actcaagaac
     4021 ggacaagcca ccgaggccaa ggagtacgag tccgtcaccg tcttcttctc cgacatcacc
     4081 aacttcaccg tcatctcctc ccgtacctcc accaaggtaa gtttaaacat gattttacta
     4141 actaactaat ctgatttaaa ttttcaggac atgatggcca ccctcaacaa gctctggctc
     4201 gagtacgacg ccatcgccaa gcgttgggga gtctacaagg tcgagaccat cggagacgcc
     4261 tacctcggag tcaccggagc cccagacgtc gtcccagacc acgccgagcg tgcctgcaac
     4321 ttcgccgtcg acatcatcga gatgatcaag tccttcaaga ccatcaccgg agagtccatc
     4381 aacatccgta tcggactcaa ctccggacca gtcaccgccg gagtcctcgg agacctcaac
     4441 ccacactggt gcctcgtcgg agacaccgtc aacaccgcct cccgtatgga gtccacctcc
     4501 aaggccggac acatccacat ctccgagtcc acctaccact tcatcaagtc caagttcgtc
     4561 acccaaccac tcgacgtcat ggaggtcaag ggaaagggaa agatgcaaac ctactgggtc
     4621 ctcggacgta agtaaggtac cgctgtctca tcctactttc acctagttaa ctgcttgtct
     4681 taaaatctat gcttctcttt agtatctaaa attttcctag aagcttacaa gtatataaat
     4741 ggtctcttct caataaaggt tgtatattta ttcatcttat tgaatctgcc atttcctcgt
     4801 ttttgcgagt ttatatacct tccaattttc tttctattgt attttcaact tctaatttta
     4861 attcagggaa actgctgtac cggtagaaaa aatgctcgag atggtgagca agggcgagga
     4921 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     4981 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     5041 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     5101 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     5161 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     5221 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     5281 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     5341 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     5401 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     5461 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     5521 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     5581 caccggcggc atggacgagc tgtacaagta agaattccaa ctgagcgccg gtcgctacca
     5641 ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt aagtttaaac
     5701 tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc cgtgcctctg
     5761 acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg tgctcccacc
     5821 ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta gcttctttta
     5881 agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg taataaaaag
     5941 tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa atctacacaa
     6001 tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa acatcataga
     6061 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
     6121 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
     6181 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
     6241 gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt ttcttgctaa
     6301 aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg ggtttgaggc
     6361 tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta tggggttttt
     6421 gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct actagtcggc
     6481 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     6541 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     6601 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     6661 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     6721 aataccgcat caggcggcct taattaaggg cctcgtgata cgcctatttt tataggttaa
     6781 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7501 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7621 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8101 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8341 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8761 gcctc
//
