LOCUS       sequence-332391-        9273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Dailamy A, Parekh U, Katrekar D, Kumar A, McDonald D, Moreno A, 
            Bagheri P, Ng TN, Mali P
  TITLE     Programmatic introduction of parenchymal cell types into blood 
            vessel organoids.
  JOURNAL   Stem Cell Reports. 2021 Sep 8. pii: S2213-6711(21)00432-X. doi: 
            10.1016/j.stemcr.2021.08.014.
  PUBMED    34559998
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Sep 8. pii: S2213-6711(21)00432-X. doi: 
            10.1016/j.stemcr.2021.08.014."
COMMENT     Sequence Label: PB_iMYOD1_P2A_GFP_Puro
FEATURES             Location/Qualifiers
     source          1..9273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   744..806
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1255..1633
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    1263..1281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1299..1317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1335..1353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1371..1389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1407..1425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1443..1461
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1479..1497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1542..1566
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1646..1655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2621..2677
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2678..2680,2681..2683,2684..3397)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2678..2680,2681..2683,2684..3394)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2723..2744)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2984..3003)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3331..3352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3434..3453)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3478..3526
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3757..3968
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3981..4249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4283..5026
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             5027..5080
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5081..5680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5081..5100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5577..5597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5713..5834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5750..5769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5804..5823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6597..6631
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7122..7138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7135..7157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7146..7162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7170..7176,7177..7194,7195..7200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7215..7236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7353..7370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7524..8112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7604..7623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8283..9074,9075..9143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8906..8925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9144..9248)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc
      661 agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc
      721 tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg tacgttaaag
      781 ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      841 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      901 caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa aatttcttct
      961 ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag ggatgtaatt
     1021 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg
     1081 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc
     1141 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg
     1201 gatacgggga aaaggcctcc acggccacta gtctttcgtc ttcaagaatt cctcgagttt
     1261 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
     1321 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
     1381 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
     1441 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
     1501 tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt
     1561 cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc
     1621 ctaccctcgt aaaggtctag agctagccac catggagcta ctgtcgccac cgctccgcga
     1681 cgtagacctg acggcccccg acggctctct ctgctccttt gccacaacgg acgacttcta
     1741 tgacgacccg tgtttcgact ccccggacct gcgcttcttc gaagacctgg acccgcgcct
     1801 gatgcacgtg ggcgcgctcc tgaaacccga agagcactcg cacttccccg cggcggtgca
     1861 cccggccccg ggcgcacgtg aggacgagca tgtgcgcgcg cccagcgggc accaccaggc
     1921 gggccgctgc ctactgtggg cctgcaaggc gtgcaagcgc aagaccacca acgccgaccg
     1981 ccgcaaggcc gccaccatgc gcgagcggcg ccgcctgagc aaagtaaatg aggcctttga
     2041 gacactcaag cgctgcacgt cgagcaatcc aaaccagcgg ttgcccaagg tggagatcct
     2101 gcgcaacgcc atccgctata tcgagggcct gcaggctctg ctgcgcgacc aggacgccgc
     2161 gccccctggc gccgcagccg ccttctatgc gccgggcccg ctgcccccgg gccgcggcgg
     2221 cgagcactac agcggcgact ccgacgcgtc cagcccgcgc tccaactgct ccgacggcat
     2281 gatggactac agcggccccc cgagcggcgc ccggcggcgg aactgctacg aaggcgccta
     2341 ctacaacgag gcgcccagcg aacccaggcc cgggaagagt gcggcggtgt cgagcctaga
     2401 ctgcctgtcc agcatcgtgg agcgcatctc caccgagagc cctgcggcgc ccgccctcct
     2461 gctggcggac gtgccttctg agtcgcctcc gcgcaggcaa gaggctgccg cccccagcga
     2521 gggagagagc agcggcgacc ccacccagtc accggacgcc gccccgcagt gccctgcggg
     2581 tgcgaacccc aacccgatat accaggtgct cggatccggc gcaacaaact tctctctgct
     2641 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gtgagcaagg gcgaggagct
     2701 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2761 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2821 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     2881 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2941 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3001 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3061 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3121 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     3181 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3241 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3301 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3361 cgggatcact ctcggcatgg acgagctgta caagtaaacc ggttagtaat gagtttaaac
     3421 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3481 caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt
     3541 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     3601 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     3661 gccaacgtcg gggcggcagg ccctgccata gcagatctgc gaaatcggat ccagctgaat
     3721 ctaagtggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
     3781 tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg
     3841 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     3901 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     3961 gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct
     4021 gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct
     4081 gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg
     4141 cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg
     4201 ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatct aagctgtgac
     4261 cggcgcctac tctagagccg ccatgtctag actggacaag agcaaagtca taaactctgc
     4321 tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     4381 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     4441 tgccctgcca atcgagatgc tggacaggca tcatacccac tcctgccccc tggaaggcga
     4501 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     4561 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     4621 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     4681 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     4741 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgaaacaagc
     4801 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     4861 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     4921 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     4981 gcctgctgac gctcttgacg attttgacct tgacatgctc cccggggagg gccgaggaag
     5041 tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt acaagcccac
     5101 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     5161 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     5221 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     5281 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     5341 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     5401 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     5461 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     5521 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     5581 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     5641 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga aatcaacctc tggattacaa
     5701 aatttgtggg taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5821 atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag
     5881 ctcatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa acagcagtgt
     5941 tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg tttcctgtcc
     6001 tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg gaattgactg
     6061 gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca ggtgtctgca
     6121 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     6181 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     6241 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     6301 gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat aataagttat
     6361 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca
     6421 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga
     6481 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta
     6541 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg
     6601 cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt
     6661 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
     6721 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
     6781 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
     6841 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc
     6901 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
     6961 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
     7021 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat
     7081 accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     7141 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7201 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7261 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7321 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7921 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     7981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8161 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8221 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8281 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8341 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8401 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8461 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8521 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8581 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8641 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8701 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8761 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8821 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8881 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8941 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9001 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9061 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9121 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9181 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9241 gttccgcgca catttccccg aaaagtgcca cct
//
