LOCUS       sequence-33241-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL EPSM
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(709..732)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(750..768)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(811..830)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 ggccgcttag taggtaactg gctgcaggtc caagttatta tgtgctgggg ccatcggatt
       61 cggggctagg tttggagcac ctgcgtccat gctgtggttc atctggtggt cactggtttc
      121 tccatcttca ctcaggtagc cagggggagg gacctctgga atattgctct ggggctcgat
      181 gcctgcgggg aagttagtgt tttcggggat ggaatggctg tagtcgtcca gtggggggaa
      241 ctcggccggg atctctgtgt ggcgtggcac caacacagga ggtagaactg gtgtctctac
      301 tctctggtag tggtagggat tcacgcagac ctcgtccttc ttcatattga aggcgaactc
      361 acacagctcc atggcccgca gctcgtggtg gctgtgcagg tctggccatc gccacaggcg
      421 gcagtagatg acatgaggga gccccttccg atgggacacc tgcaaccggc catccaggga
      481 cctggggatg gtgatgcact tggtgttgac gttctgcgtg gtgatggcct tctccagctc
      541 gtccagctgc cccgtcttct tgagtttctt gaccaggctc ttgaccgcct tctcgcacca
      601 tttctcctcc tgcccgttct gctcgccctt cttccagccc agcaggcgct tcacgatcgg
      661 gggagtgaaa ggcaggatgg acgatctaga ggtaccacgc gtgaattctt tgtcatcgtc
      721 atccttatag tccatggtct cgaggctagc ctatagtgag tcgtattaag tactctagcc
      781 ttaagagctg taattgaact gggagtggac acctgtggag agaaaggcaa agtggatgtc
      841 agtaagacca ataggt
//
