LOCUS       sequence-332419-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon construct with doxycycline inducible MYOD1, 
            BAF60C + mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Dailamy A, Parekh U, Katrekar D, Kumar A, McDonald D, Moreno A, 
            Bagheri P, Ng TN, Mali P
  TITLE     Programmatic introduction of parenchymal cell types into blood 
            vessel organoids.
  JOURNAL   Stem Cell Reports. 2021 Sep 8. pii: S2213-6711(21)00432-X. doi: 
            10.1016/j.stemcr.2021.08.014.
  PUBMED    34559998
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Sep 8. pii: S2213-6711(21)00432-X. doi: 
            10.1016/j.stemcr.2021.08.014."
COMMENT     Sequence Label: PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             568..624
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2074..2127
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2128..2838
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2273..2291)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2541..2560
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2875..2894)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2919..2967
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3198..3409
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3422..3690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3724..4467
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             4468..4521
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4522..5121
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4522..4541)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5018..5038
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5154..5275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5191..5210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5245..5264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6038..6072
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6563..6579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6563..6579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6576..6598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6587..6603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6611..6617,6618..6635,6636..6641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6656..6677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6794..6811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8715..9170)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8852..8873)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9064..9083
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9297..9319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9311..9328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9312..9328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   9458..9520
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        9969..10347
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    9977..9995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10013..10031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10049..10067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10085..10103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10121..10139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10157..10175
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10193..10211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10256..10280
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10360..10369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgcacgtcga gcaatccaaa ccagcggttg cccaaggtgg agatcctgcg caacgccatc
       61 cgctatatcg agggcctgca ggctctgctg cgcgaccagg acgccgcgcc ccctggcgcc
      121 gcagccgcct tctatgcgcc gggcccgctg cccccgggcc gcggcggcga gcactacagc
      181 ggcgactccg acgcgtccag cccgcgctcc aactgctccg acggcatgat ggactacagc
      241 ggccccccga gcggcgcccg gcggcggaac tgctacgaag gcgcctacta caacgaggcg
      301 cccagcgaac ccaggcccgg gaagagtgcg gcggtgtcga gcctagactg cctgtccagc
      361 atcgtggagc gcatctccac cgagagccct gcggcgcccg ccctcctgct ggcggacgtg
      421 ccttctgagt cgcctccgcg caggcaagag gctgccgccc ccagcgaggg agagagcagc
      481 ggcgacccca cccagtcacc ggacgccgcc ccgcagtgcc ctgcgggtgc gaaccccaac
      541 ccgatatacc aggtgctcgg atccggcgca acaaacttct ctctgctgaa acaagccgga
      601 gatgtcgaag agaatcctgg accgatggcc gcggacgaag ttgccggagg ggcgcgcaaa
      661 gccacgaaaa gcaaactttt tgagtttctg gtccatgggg tgcgccccgg gatgccgtct
      721 ggagcccgga tgccccacca gggggcgccc atgggccccc cgggctcccc gtacatgggc
      781 agccccgccg tgcgacccgg cctggccccc gcgggcatgg agcccgcccg caagcgagca
      841 gcgcccccgc ccgggcagag ccaggcacag agccagggcc agccggtgcc caccgccccc
      901 gcgcggagcc gcagtgccaa gaggaggaag atggctgaca aaatcctccc tcaaaggatt
      961 cgggagctgg tccccgagtc ccaggcttac atggacctct tggcatttga gaggaaactg
     1021 gatcaaacca tcatgcggaa gcgggtggac atccaggagg ctctgaagag gcccatgaag
     1081 caaaagcgga agctgcgact ctatatctcc aacactttta accctgcgaa gcctgatgct
     1141 gaggattccg acggcagcat tgcctcctgg gagctacggg tggaggggaa gctcctggat
     1201 gatcccagca aacagaagcg gaagttctct tctttcttca agagtttggt catcgagctg
     1261 gacaaagatc tttatggccc tgacaaccac ctcgttgagt ggcatcggac acccacgacc
     1321 caggagacgg acggcttcca ggtgaaacgg cctggggacc tgagtgtgcg ctgcacgctg
     1381 ctcctcatgc tggactacca gcctccccag ttcaaactgg atccccgcct agcccggctg
     1441 ctggggctgc acacacagag ccgctcagcc attgtccagg ccctgtggca gtatgtgaag
     1501 accaacaggc tgcaggactc ccatgacaag gaatacatca atggggacaa gtatttccag
     1561 cagatttttg attgtccccg gctgaagttt tctgagattc cccagcgcct cacagccctg
     1621 ctattgcccc ctgacccaat tgtcatcaac catgtcatca gcgtggaccc ttcagaccag
     1681 aagaagacgg cgtgctatga cattgacgtg gaggtggagg agccattaaa ggggcagatg
     1741 agcagcttcc tcctatccac ggccaaccag caggagatca gtgctctgga cagtaagatc
     1801 catgagacga ttgagtccat aaaccagctc aagatccaga gggacttcat gctaagcttc
     1861 tccagagacc ccaaaggcta tgtccaagac ctgctccgct cccagagccg ggacctcaag
     1921 gtgatgacag atgtagccgg caaccctgaa gaggagcgcc gggctgagtt ctaccaccag
     1981 ccctggtccc aggaggccgt cagtcgctac ttctactgca agatccagca gcgcaggcag
     2041 gagctggagc agtcgctggt tgtgcgcaac accgaaggta gaggctcact gttaacgtgt
     2101 ggcgatgtag aagagaatcc aggacctatg gtgagcaagg gcgaggagga taacatggcc
     2161 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     2221 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     2281 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     2341 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     2401 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     2461 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     2521 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     2581 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     2641 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     2701 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     2761 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     2821 gacgagctgt acaagtaaac cggttagtaa tgagtttaaa cgggggaggc taactgaaac
     2881 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     2941 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     3001 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     3061 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     3121 gccctgccat agcagatctg cgaaatcgga tccagctgaa tctaagtgga tctgcgatcg
     3181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     3241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     3301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     3361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
     3421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
     3481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
     3541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
     3601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
     3661 ttcgttttct gttctgcgcc gttacagatc taagctgtga ccggcgccta ctctagagcc
     3721 gccatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga
     3781 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     3841 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     3901 ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg
     3961 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     4021 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     4081 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     4141 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     4201 acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg
     4261 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     4321 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     4381 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     4441 gattttgacc ttgacatgct ccccggggag ggccgaggaa gtcttctaac atgcggtgac
     4501 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     4561 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     4621 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     4681 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     4741 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     4801 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     4861 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     4921 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     4981 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     5041 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     5101 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtgg gtaaacttgt
     5161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5281 tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg tctcccttcc
     5341 gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact gggttcttat
     5401 atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct
     5461 aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt tttagggccc
     5521 attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     5581 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     5641 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     5701 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     5761 cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     5821 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     5881 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     5941 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     6001 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     6061 atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt tttccggctc
     6121 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc
     6181 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     6241 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     6301 ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt ccggacacag
     6361 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
     6421 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
     6481 gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc
     6541 gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6601 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6661 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6721 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     8761 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     8821 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     8881 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     8941 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9001 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9061 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     9121 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     9181 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     9241 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     9301 gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg tttttgaacc
     9361 cgtggaggac gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa
     9421 gatgctcgac acgctgcaga acacgcagct agattaaccc tagaaagata atcatattgt
     9481 gacgtacgtt aaagataatc atgtgtaaaa ttgacgcatg tgttttatcg gtctgtatat
     9541 cgaggtttat ttattaattt gaatagatat taagttttat tatatttaca cttacatact
     9601 aataataaat tcaacaaaca atttatttat gtttatttat ttattaaaaa aaacaaaaac
     9661 tcaaaatttc ttctataaag taacaaaact tttatgaggg acagcccccc cccaaagccc
     9721 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     9781 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     9841 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     9901 cagacacctg gggggatacg gggaaaaggc ctccacggcc actagtcttt cgtcttcaag
     9961 aattcctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
    10021 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
    10081 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
    10141 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
    10201 agtgatagag aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc
    10261 gtttagtgaa ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact
    10321 ttccgtacca cttcctaccc tcgtaaaggt ctagagctag ccaccatgga gctactgtcg
    10381 ccaccgctcc gcgacgtaga cctgacggcc cccgacggct ctctctgctc ctttgccaca
    10441 acggacgact tctatgacga cccgtgtttc gactccccgg acctgcgctt cttcgaagac
    10501 ctggacccgc gcctgatgca cgtgggcgcg ctcctgaaac ccgaagagca ctcgcacttc
    10561 cccgcggcgg tgcacccggc cccgggcgca cgtgaggacg agcatgtgcg cgcgcccagc
    10621 gggcaccacc aggcgggccg ctgcctactg tgggcctgca aggcgtgcaa gcgcaagacc
    10681 accaacgccg accgccgcaa ggccgccacc atgcgcgagc ggcgccgcct gagcaaagta
    10741 aatgaggcct ttgagacact caagcgc
//
