LOCUS       sequence-33243-e         374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Alarcon C, Zaromytidou AI, Xi Q, Gao S, Yu J, Fujisawa S, Barlas A, 
            Miller AN, Manova-Todorova K, Macias MJ, Sapkota G, Pan D, Massague 
            J
  TITLE     Nuclear CDKs drive Smad transcriptional activation and turnover in 
            BMP and TGF-beta pathways.
  JOURNAL   Cell. 2009 Nov 13. 139(4):757-69.
  PUBMED    19914168
REFERENCE   2  (bases 1 to 374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Nov 13. 139(4):757-69."
COMMENT     Sequence Label: pCl-HA-YAP
FEATURES             Location/Qualifiers
     source          1..374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          89..221
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     204..223
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        266..284
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             305..331
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcacagtta attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg
       61 cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
      121 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      181 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa
      241 ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc tcgagaccat
      301 ggattatccg tatgatgttc ctgattatgc agaattcacg cgtggtacct ctagaatgga
      361 tcccgggcag caga
//
