LOCUS       sequence-332434-       11897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full 
            length human O-GlcNAc transferase, D554N variant, no Ser/Thr 
            glycosylation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara
            NE, Gray NS, Paulo JA, Walker S
  TITLE     Mammalian cell proliferation requires noncatalytic functions of 
            O-GlcNAc transferase.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2016778118. doi: 
            10.1073/pnas.2016778118.
  PUBMED    33419956
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2016778118. doi"; pages: " 10.1073/pnas.2016778118"
COMMENT     Sequence Label: pLenti PGK mKate2-OGT D554N (Puro)
FEATURES             Location/Qualifiers
     source          1..11897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    44..161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        207..717
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     636..654
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    772..796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             827..1522
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             1529..1552
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1556..1579
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    1595..1619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4744..4768)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4799..5387
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4852..4872)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5270..5281)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5401..5900
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5436..5459)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5833..5852
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5921..6520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5921..5940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6417..6437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6658..6891
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6963..7084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7000..7019)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7054..7073
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7124..7259
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7279..7298)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7280..7298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7308..7325)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7308..7324)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7317..7339)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7466..7921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7553..7572)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7763..7784
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7947..8051
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8052..8120,8121..8912)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8270..8289)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9083..9671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9572..9591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9825..9842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9959..9980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9995..10000,10001..10018,10019..10025)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10033..10049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10038..10060
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10057..10073
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10057..10073
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10092..10112
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10094..10112
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10140..10366
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10367..10547
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10594..10719
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11212..11445
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11630..11674
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg
       61 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      121 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc
      181 tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca aggcagccct
      241 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
      301 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
      361 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
      421 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
      481 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
      541 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
      601 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
      661 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
      721 gatccaccgg ttcgtcgact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      781 tacaaaaaag caggctttaa aggaaccaat tcagtcgact ggatccatgg tgagcgagct
      841 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
      901 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
      961 ggcggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1021 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     1081 ccccgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1141 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1201 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
     1261 cgagaccctg taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct
     1321 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc
     1381 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga
     1441 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     1501 ccctagcaaa ctggggcaca gaggcggaga ctacaaagat gatgacgaca aaggcgacta
     1561 caaagacgat gacgacaagg gcatcgatag atcaacaagt ttgtacaaaa aagcaggctt
     1621 tgcgtcttcc gtgggcaacg tggccgacag cacagggtta gctgagttgg cacatcgaga
     1681 atatcaggca ggagattttg aggcagctga gagacactgc atgcagctct ggagacaaga
     1741 gccagacaat actggtgtgc ttttattact ttcatctata cacttccagt gtcgaaggct
     1801 ggacagatct gctcacttta gcactctggc aattaaacag aacccccttc tggcagaagc
     1861 ttattcgaat ttggggaatg tgtacaagga aagagggcag ttgcaggagg caattgagca
     1921 ttatcgacat gcattgcgtc tcaaacctga tttcatcgat ggttatatta acctggcagc
     1981 cgccttggta gcagcgggtg acatggaagg ggcagtacaa gcttacgtct ctgctcttca
     2041 gtacaatcct gatttgtact gtgttcgcag tgacctgggg aacctgctca aagccctggg
     2101 tcgcttggaa gaagccaagg catgttattt gaaagcaatt gagacgcaac cgaactttgc
     2161 agtagcttgg agtaatcttg gctgtgtttt caatgcacaa ggggaaattt ggcttgcaat
     2221 tcatcacttt gaaaaggctg tcacccttga cccaaacttt ctggatgctt atatcaattt
     2281 aggaaatgtc ttgaaagagg cacgcatttt tgacagagct gtggcagctt atcttcgtgc
     2341 cctaagtttg agtccaaatc acgcagtggt gcacggcaac ctggcttgtg tatactatga
     2401 gcaaggcctg atagatctgg caatagacac ctacaggcgg gctatcgaac tacaaccaca
     2461 tttccctgat gcttactgca acctagccaa tgctctcaaa gagaagggca gtgttgctga
     2521 agcagaagat tgttataata cagctctccg tctgtgtccc acccatgcag actctctgaa
     2581 taacctagcc aatatcaaac gagaacaggg aaacattgaa gaggcagttc gcttgtatcg
     2641 taaagcatta gaagtcttcc cagagtttgc tgctgcccat tcaaatttag caagtgtact
     2701 gcagcagcag ggaaaactgc aggaagctct gatgcattat aaggaggcta ttcgaatcag
     2761 tcctaccttt gctgatgcct actctaatat gggaaacact ctaaaggaga tgcaggatgt
     2821 tcagggagcc ttgcagtgtt atacgcgtgc catccaaatt aatcctgcat ttgcagatgc
     2881 acatagcaat ctggcttcca ttcataagga ttcagggaat attccagaag ccatagcttc
     2941 ttaccgcacg gctctgaaac ttaagcctga ttttcctgat gcttattgta acttggctca
     3001 ttgcctgcag attgtctgtg attggacaga ctatgatgag cgaatgaaga agttggtcag
     3061 tattgtggct gaccagttag agaagaatag gttgccttct gtgcatcctc atcatagtat
     3121 gctatatcct ctttctcatg gcttcaggaa ggctattgct gagaggcacg gcaacctgtg
     3181 cttagataag attaatgttc ttcataaacc accatatgaa catccaaaag acttgaagct
     3241 cagtgatggt cggctgcgtg taggatatgt gagttccaac tttgggaatc atcctacttc
     3301 tcaccttatg cagtctattc caggcatgca caatcctgat aaatttgagg tgttctgtta
     3361 tgccctgagc ccagacgatg gcacaaactt ccgagtgaag gtgatggcag aagccaatca
     3421 tttcattgat ctttctcaga ttccatgcaa tggaaaagca gctgatcgca tccatcagga
     3481 tggaattcat atccttgtaa atatgaatgg ctatactaag ggcgctcgaa atgagctttt
     3541 tgctctcagg ccagctccta ttcaggcaat gtggctggga taccctggga cgagtggtgc
     3601 gcttttcatg gattatatta tcactgatca ggaaacttcg ccagctgaag ttgctgagca
     3661 gtattccgag aaattggctt atatgcccca cacttttttt attggtgatc atgctaatat
     3721 gttccctcac ctgaagaaaa aagcagtcat cgattttaag tccaatgggc acatttatga
     3781 caatcggata gttctgaatg gcatcgacct caaagcattt cttgatagtc taccagatgt
     3841 gaaaattgtc aagatgaagt gtcctgatgg aggagacaat gcagatagca gtaacacagc
     3901 tcttaatatg cctgttattc ctatgaatac tattgcagaa gcagttattg aaatgattaa
     3961 ccgaggacag attcaaataa caattaatgg attcagtatt agcaatggac tggcaactac
     4021 tcagatcaac aataaggctg caactggaga ggaggttccc cgtaccatta ttgtaaccac
     4081 ccgttctcag tacgggttac cagaagatgc catcgtatac tgtaacttta atcagttgta
     4141 taaaattgac ccttctactt tgcagatgtg ggcaaacatt ctgaagcgtg ttcccaatag
     4201 tgtactctgg ctgttgcgtt ttccagcagt aggagaacct aatattcaac agtatgcaca
     4261 aaacatgggc ctgccccaga accgtatcat tttttcacct gttgctccta aagaggaaca
     4321 cgtcaggaga ggccagctgg ctgatgtctg cttggacact ccactctgta atgggcacac
     4381 cacagggatg gatgtcctct gggcagggac ccccatggtg actatgccag gagagactct
     4441 tgcttctcga gttgcagcat cccagctcac ttgcttaggt tgtcttgagc ttattgctaa
     4501 aaacagacaa gaatatgaag acatagctgt gaagctggga actgatctag aatacctgaa
     4561 gaaagttcgt ggcaaagtct ggaagcaaag aatatctagc cctctgttca acaccaaaca
     4621 atacacaatg gaactagagc ggctctatct acagatgtgg gagcattatg cagctggcaa
     4681 caaacctgac cacatgatta agcctgttga agtcactgag tcagcataac tcgagatatc
     4741 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgacaa
     4801 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4861 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4921 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4981 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5041 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5101 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5161 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     5221 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5281 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5341 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc
     5401 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     5461 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     5521 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     5581 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     5641 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     5701 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     5761 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     5821 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     5881 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     5941 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6001 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6061 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6121 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6181 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6241 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6301 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6361 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6421 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6481 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     6541 gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac
     6601 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6661 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6721 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6781 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6841 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6901 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6961 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7021 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7081 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7141 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7201 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7261 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     7321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7441 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     7501 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     7561 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     7621 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     7681 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     7741 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     7801 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     7861 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     7921 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7981 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8041 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8101 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8161 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8221 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8281 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8341 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8401 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8461 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8521 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8581 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8641 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8701 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8761 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8821 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8881 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8941 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9001 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9061 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9121 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9181 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9241 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9301 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9361 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9421 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9481 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9541 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9601 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9661 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9721 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9781 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9841 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9901 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9961 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10021 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10081 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
    10141 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
    10201 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
    10261 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
    10321 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
    10381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
    10441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
    10501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
    10561 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
    10621 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
    10681 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
    10741 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
    10801 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
    10861 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
    10921 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
    10981 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
    11041 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11101 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11161 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    11221 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    11281 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    11341 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    11401 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    11461 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    11521 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    11581 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    11641 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    11701 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    11761 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    11821 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    11881 agagagagac agagaca
//
