LOCUS       sequence-332447-       13388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for NPC1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Davis OB, Shin HR, Lim CY, Wu EY, Kukurugya M, Maher CF, Perera RM, 
            Ordonez MP, Zoncu R
  TITLE     NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle 
            Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C.
  JOURNAL   Dev Cell. 2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016.
  PUBMED    33308480
REFERENCE   2  (bases 1 to 13388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2020 Dec 7. pii: S1534-5807(20)30925-4. doi: 
            10.1016/j.devcel.2020.11.016."
COMMENT     Sequence Label: pLVX-NPC1(F503/4A)-FLAG
FEATURES             Location/Qualifiers
     source          1..13388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    23..138
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        332..1514
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          563..1505
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1462..1482
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5420..5443
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5475..6194
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     primer_bind     complement(6257..6280)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6650..6669
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6738..7337
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6738..6757)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7234..7254
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7351..7939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7404..7424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7822..7833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8146..8779
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8908..8924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8908..8924)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8921..8943)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8932..8948
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8956..8962,8963..8980,8981..8986))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9001..9022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9139..9156)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9310..9898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9390..9409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10069..10860,10861..10929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10692..10711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10930..11034)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11082..11216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11119..11138)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11173..11192
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             11384..12017
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12064..12189
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12686..12919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13104..13148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aacggatctc gacggtatcg cctttaaaag aaaagggggg attggggggt acagtgcagg
       61 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      121 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttatcgatg
      181 agtaattcat acaaaaggac tcgcccctgc cttggggaat cccagggacc gtcgttaaac
      241 tcccactaac gtagaaccca gagatcgctg cgttcccgcc ccctcacccg cccgctctcg
      301 tcatcactga ggtggagaag agcatgcgtg aggctccggt gcccgtcagt gggcagagcg
      361 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta
      421 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
      481 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
      541 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
      601 tacgggttat ggcccttgcg tgccttgaat tacttccacg cccctggctg cagtacgtga
      661 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      721 gagccccttc gcctcgtgct tgagttgagg cctggcttgg gcgctggggc cgccgcgtgc
      781 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      841 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      901 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      961 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     1021 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     1081 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     1141 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1201 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1261 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1321 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1381 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1441 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1501 ttcaggtgtc gtgaggatcg ctagcgctac cggactcaga tctcgagctc aagcttcgaa
     1561 ttggatccac gtggaattcg ccaccatgac cgctagaggg ctggctctgg ggctgctgct
     1621 gctgctgctg tgccccgctc aggtgttcag tcagtcttgc gtgtggtatg gcgagtgcgg
     1681 catcgcctat ggggacaaga ggtataactg tgaatactct ggacccccta agccactgcc
     1741 caaagacggc tacgatctgg tgcaggaact gtgcccagga ttctttttcg gcaacgtgag
     1801 cctgtgctgt gacgtgcggc agctgcagac cctgaaggat aatctgcagc tgcctctgca
     1861 gtttctgtcc aggtgcccat cttgttttta taacctgctg aatctgttct gcgagctgac
     1921 ctgtagtcca aggcagtcac agttcctgaa cgtgactgcc accgaggact acgtcgatcc
     1981 cgtgacaaac cagacaaaga ctaatgtcaa agaactgcag tactatgtgg gccagtcctt
     2041 cgccaacgct atgtataatg cttgccgaga cgtggaggca ccaagctcca atgataaggc
     2101 cctggggctg ctgtgcggaa aagacgcaga tgcctgtaac gctaccaatt ggatcgaata
     2161 catgtttaac aaggataatg gccaggcccc tttcaccatt acaccagtgt ttagcgactt
     2221 ccccgtccac gggatggagc ctatgaacaa tgccactaag ggatgcgacg agtccgtcga
     2281 tgaagtgacc gctccttgct cctgtcagga ttgctctatc gtgtgcgggc ctaaaccaca
     2341 gccaccacct ccaccagcac catggacaat tctgggactg gacgccatgt atgtgattat
     2401 gtggatcact tacatggcct ttctgctggt ctttttcggc gcatttttcg ccgtgtggtg
     2461 ttatcgaaag cggtactttg tgagcgagta cacccccatt gactccaaca tcgctttcag
     2521 cgtgaatgca tccgacaaag gcgaggcatc ctgctgtgat cccgtgagcg ccgctttcga
     2581 agggtgcctg cggagactgt ttactcgatg ggggtctttc tgcgtgcgga accccggatg
     2641 cgtgatcttc ttcagcctgg tgtttatcac cgcctgttct agtgggctgg tgttcgtccg
     2701 agtgaccaca aatccagtgg atctgtggag cgccccatca agccaggctc gactggagaa
     2761 ggaatacttt gaccagcatt tcggaccatt tttcagaaca gagcagctga tcattagggc
     2821 acccctgact gataaacaca tctaccagcc ctatccttct ggagccgacg tgcctttcgg
     2881 ccctccactg gatatccaga ttctgcatca ggtgctggac ctgcagatcg ccattgagaa
     2941 cattaccgct agctatgaca atgaaactgt gaccctgcag gatatctgcc tggctcctct
     3001 gagcccatat aacactaatt gtaccattct gtccgtcctg aactactttc agaatagcca
     3061 ctccgtgctg gatcataaga aaggcgacga cgccgccgtg tacgccgact atcacaccca
     3121 tttcctgtac tgcgtgcggg ctcctgcaag tctgaacgac acctcactgc tgcacgatcc
     3181 atgtctgggg acatttggcg gacccgtgtt cccttggctg gtcctgggag gctatgacga
     3241 tcagaactac aacaatgcca ccgccctggt catcaccttc cctgtcaaca attactataa
     3301 tgataccgag aagctgcaga gagcacaggc ctgggagaaa gagttcatca acttcgtgaa
     3361 gaattacaaa aacccaaatc tgaccattag tttcacagcc gagcggagca tcgaggacga
     3421 actgaaccga gaatctgaca gtgacgtgtt caccgtggtc attagctatg ctatcatgtt
     3481 cctgtacatc tcactggcac tgggccatat gaagagctgc aggcgcctgc tggtggattc
     3541 aaaagtcagc ctgggaattg ccggcatcct gattgtgctg tcctctgtcg cttgtagcct
     3601 gggggtgttc tcctatattg gactgcctct gaccctgatc gtcattgaag tgatcccatt
     3661 tctggtcctg gctgtcggag tggacaatat cttcattctg gtgcaggcat accagcgcga
     3721 cgagcgactg cagggcgaaa cactggatca gcagctggga agggtgctgg gagaggtcgc
     3781 tccatctatg tttctgagtt cattcagtga aaccgtggca tttttcctgg gcgccctgag
     3841 tgtgatgcca gcagtccaca cattttcact gttcgctggg ctggcagtgt ttattgactt
     3901 cctgctgcag atcacctgct ttgtgagcct gctgggcctg gacattaaga ggcaggagaa
     3961 aaaccgcctg gatatcttct gctgcgtgcg gggagcagag gacggcacat ccgtgcaggc
     4021 ttcagaaagc tgtctgttca ggtttttcaa gaacagctat tctccactgc tgctgaaaga
     4081 ctggatgcgc cccatcgtga ttgcaatctt tgtcggagtg ctgagtttct caattgccgt
     4141 gctgaacaag gtcgacatcg gcctggatca gagcctgtcc atgcccgacg attcttacat
     4201 ggtggattac tttaaatcta tcagtcagta tctgcacgcc ggcccccctg tctacttcgt
     4261 gctggaggaa ggccatgact acactagctc caaggggcag aatatggtgt gcgggggaat
     4321 gggatgtaac aatgattccc tggtgcagca gatcttcaac gcagcccagc tggacaatta
     4381 tacaaggatt ggctttgccc cctctagttg gatcgacgat tacttcgatt gggtcaagcc
     4441 tcagtcaagc tgctgtcgcg tggacaacat cacagatcag ttttgcaatg caagcgtggt
     4501 cgaccctgcc tgcgtgagat gtaggccact gactccagag ggcaaacagc gaccacaggg
     4561 aggcgacttc atgagattcc tgcctatgtt cctgtccgac aacccaaatc ccaagtgtgg
     4621 caaaggaggc cacgctgcat actcctctgc agtcaacatc ctgctgggac atgggacccg
     4681 agtgggagcc acatacttca tgacatacca cactgtgctg cagacctcag ctgacttcat
     4741 tgatgcactg aagaaagccc gcctgatcgc tagcaacgtg acagagacta tggggatcaa
     4801 tggatccgcc tatcgagtgt ttccctactc tgtcttttac gtgttctatg aacagtacct
     4861 gactatcatt gacgatacca tcttcaacct gggggtgtcc ctgggagcta tcttcctggt
     4921 cactatggtg ctgctgggct gcgagctgtg gtctgccgtg attatgtgcg ccaccatcgc
     4981 tatggtcctg gtgaatatgt tcggcgtgat gtggctgtgg gggatctctc tgaacgccgt
     5041 cagtctggtg aatctggtca tgtcttgcgg gattagtgtg gagttttgta gccatatcac
     5101 tagagctttc accgtctcaa tgaagggaag ccgggtggaa agagcagagg aagctctggc
     5161 acacatggga agttcagtgt ttagtggcat taccctgaca aagttcgggg gaatcgtggt
     5221 cctggccttt gctaagagcc agatcttcca gatcttttat ttccggatgt acctggctat
     5281 ggtgctgctg ggcgcaacac acgggctgat tttcctgccc gtcctgctgt catatatcgg
     5341 ccctagcgtg aacaaggcaa aaagctgcgc cactgaggaa agatacaagg ggactgagcg
     5401 agagagactg ctgaactttg actacaagga tgacgatgat aaataacccg ggctcatcta
     5461 gagatccacc ggtcatggtg agcaagggcg ccgagctgtt caccggcatc gtgcccatcc
     5521 tgatcgagct gaatggcgat gtgaatggcc acaagttcag cgtgagcggc gagggcgagg
     5581 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcctg
     5641 tgccctggcc caccctggtg accaccctga gctacggcgt gcagtgcttc tcacgctacc
     5701 ccgatcacat gaagcagcac gacttcttca agagcgccat gcctgagggc tacatccagg
     5761 agcgcaccat cttcttcgag gatgacggca actacaagtc gcgcgccgag gtgaagttcg
     5821 agggcgatac cctggtgaat cgcatcgagc tgaccggcac cgatttcaag gaggatggca
     5881 acatcctggg caataagatg gagtacaact acaacgccca caatgtgtac atcatgaccg
     5941 acaaggccaa gaatggcatc aaggtgaact tcaagatccg ccacaacatc gaggatggca
     6001 gcgtgcagct ggccgaccac taccagcaga atacccccat cggcgatggc cctgtgctgc
     6061 tgcccgataa ccactacctg tccacccaga gcgccctgtc caaggacccc aacgagaagc
     6121 gcgatcacat gatctacttc ggcttcgtga ccgccgccgc catcacccac ggcatggatg
     6181 agctgtacaa gtgagcggcc gcgactctag ataattctac cgggtagggg aggcgctttt
     6241 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     6301 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     6361 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     6421 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     6481 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     6541 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggctcagg
     6601 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     6661 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     6721 tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6781 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6841 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6901 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6961 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     7021 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     7081 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     7141 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     7201 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     7261 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     7321 gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     7381 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     7441 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     7501 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     7561 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     7621 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     7681 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     7741 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7801 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7861 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7921 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     7981 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     8041 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     8101 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     8161 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     8221 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     8281 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     8341 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     8401 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     8461 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     8521 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     8581 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8641 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8701 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8761 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8821 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac
     8881 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8941 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9001 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9061 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9121 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     9301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     9361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    10321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    10381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    10441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11041 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
    11101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11221 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11281 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11341 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    11401 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    11461 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11521 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11581 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    11641 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    11701 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    11761 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    11821 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    12001 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    12061 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    12121 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    12181 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    12241 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    12301 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    12361 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    12421 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12481 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    12541 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    12601 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    12661 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    12721 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    12781 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    12841 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    12901 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    12961 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    13021 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    13081 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    13141 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    13201 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    13261 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    13321 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    13381 gattagtg
//
