LOCUS       sequence-332484-        2997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mActZ-FY, a FiCE of iCMM, in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Miyamoto T, Uosaki H, Mizunoe Y, Han SI, Goto S, Yamanaka D, Masuda 
            M, Yoneyama Y, Nakamura H, Hattori N, Takeuchi Y, Ohno H, Sekiya M, 
            Matsuzaka T, Hakuno F, Takahashi SI, Yahagi N, Ito K, Shimano H
  TITLE     Rapid manipulation of mitochondrial morphology in a living cell with
            iCMM.
  JOURNAL   Cell Rep Methods. 2021 Jul 22;1(4):100052. doi: 
            10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23.
  PUBMED    35475143
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-07-22"; volume: "1(4):100052. doi"; pages: " 
            10.1016/j.crmeth.2021.100052. eCollection 2021 Aug 23"
COMMENT     Sequence Label: mActZ-FY
FEATURES             Location/Qualifiers
     source          1..2997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1939..2259
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(2281..2283,2284..2997)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2323..2344)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2584..2603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2931..2952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggctacag attcagagga tagctccctg aacacagacg agtgggagga ggagaagacc
       61 gaggagcagc cctccgaagt gaatacaggc cctagatacg agaccgccag agaggtgtct
      121 agccgggaca tcaaggagct ggagaagtct aacaaggtgc gcaacacaaa taaggccgat
      181 ctgatcgcca tgctgaagga gaaggccgag aagggcccaa atatcaacaa taacaattcc
      241 gagcagaccg agaacgccgc catcaatgag gaggcctctg gagcagacag gccagcaatc
      301 caggtggaga ggagacaccc aggactgcca tccgattctg ccgcagagat caagaagcgg
      361 cgcaaggcca tcgcctcctc tgactctgag ctggagagcc tgacatatcc tgataagcca
      421 accaaggtga acaagaagaa ggtggcaaag gagagcgtgg cagacgccag cgagtccgac
      481 ctggatagct ccatgcagtc cgccgatgag tctagcccac agcccctgaa ggccaaccag
      541 cagcccttct ttcctaaggt gttcaagaag atcaaggacg ccggcaagtg ggtgcgcgac
      601 aagatcgatg agaatcccga ggtgaagaag gccatcgtgg acaagagcgc cggcctgatc
      661 gatcagctgc tgaccaagaa gaagagcgag gaggtgaacg ccgcggcccc ccgcccgtct
      721 cccgcgatct ccgtttcggt ctcggctccg gctttttacg ccccgcagaa gaagttcggc
      781 cctgtggtgg ccccaaagcc caaagtgaat cccttccggc ccggggacag cgagcctccc
      841 ccggcacccg gggcccagcg cgcacagatg ggccgggtgg gcgagattcc cccgccgccc
      901 ccggaagact ttcccctgcc tccacctccc cttgctgggg atggcgacga tgcagagggt
      961 gctctgggag gtgccttccc gccgccccct cccccgatcg aggaatcatt tccccctgcg
     1021 cctctggagg aggagatctt cccttccccg ccgcctcctc cggaggagga gggagggcct
     1081 gaggccccca taccgccccc accacagccc agggagaagg tgagcagtat tgatttggag
     1141 atcgactctc tgtcctcact gctggatgac atgaccaaga atgatccttt caaagcccgg
     1201 gtgtcatctg gatatgtgcc cccaccagtg gccactccat tcagttccaa gtccagtacc
     1261 aagcctgcag ccgggggcac agcacccctg cctccttgga agtccccttc cagctcccag
     1321 cctctgcccc aggttccggc tccggctcag agccagacac agttccatgt tcagccccag
     1381 ccccagccca agcctcaggt ccaactccat gtccagtccc agacccagcc tgtgtctttg
     1441 gctaacaccc agccccgagg gcccccagcc tcatctccgg ctccagcccc taagttttct
     1501 ccagtgactc ctaagtttac tcctgtggct tccaagttca gtcctggagc cccaggtgga
     1561 tctgggtcac aaccaaatca aaaattgggg caccccgaag ctctttctgc tggcacaggc
     1621 tcccctcaac ctcccagctt cacctatgcc cagcagaggg agaagccccg agtgcaggag
     1681 aagcagcacc ccgtgccccc accggctcag aaccaaaacc aggtgcgctc ccctggggcc
     1741 ccagggcccc tgactctgaa ggaggtggag gagctggagc agctgaccca gcagctaatg
     1801 caggacatgg agcatcctca gaggcagaat gtggctgtcc gaattctgca gtcgacggta
     1861 ccgcgggccc gggatccaag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     1921 ggtggtagtg ctggtggtgg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc
     1981 ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag
     2041 aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag
     2101 gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg
     2161 actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat
     2221 gccactctcg tcttcgatgt ggagcttcta aaactggaaa gtgctggtgg tccaccggtc
     2281 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2341 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2401 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2461 gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag
     2521 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2581 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2641 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2701 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2761 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2821 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2881 ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2941 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa
//
