LOCUS       sequence-33249-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pRS-puro shNEDD4L #2
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     41..57
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(130..344)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(190..209)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        355..854
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(390..413)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     787..806
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(879..898)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atcgatctct cgaggtcgac ggtatcgata
       61 agcttttcca aaaatgagga tcatttgtcc tactctcttg aagtaggaca aatgatcctc
      121 aggggatctg tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      181 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
      241 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
      301 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag
      361 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      421 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      481 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      541 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      601 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      661 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      721 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
      781 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
      841 ctccgggcct ttcgacctgc agcccaagct agcttaccat gaccgagtac aagcccacgg
      901 tgcgcctcgc cacccgcgac gacgtcccc
//
