LOCUS       sequence-33250-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Nguyen DX, Chiang AC, Zhang XH, Kim JY, Kris MG, Ladanyi M, Gerald 
            WL, Massague J
  TITLE     WNT/TCF signaling through LEF1 and HOXB9 mediates lung 
            adenocarcinoma metastasis.
  JOURNAL   Cell. 2009 Jul 10. 138(1):51-62.
  PUBMED    19576624
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 10. 138(1):51-62."
COMMENT     Sequence Label: MSCV-PM mir shLEF1 b
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     122..141
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           254..375
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           403..417
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           445..571
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(620..643)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
       61 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      121 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      181 gtggaaagga cgaaacaccg tgctcgcttc ggcagcacat atactagtcg actagggata
      241 acagggtaat tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
      301 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat
      361 tgctgttgac agtgagcgcc gagttattcc gggtacataa tagtgaagcc acagatgtat
      421 tatgtacccg gaataactcg atgcctactg cctcggaatt caaggggcta ctttaggagc
      481 aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc
      541 tgaattaaaa tggtataaat taaatcactt ttttcaattc taccgggtag gggaggcgct
      601 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
      661 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
      721 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
      781 cccgcagctc gcgtcgtgca gga
//
