LOCUS       sequence-33251-e         540 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Nguyen DX, Chiang AC, Zhang XH, Kim JY, Kris MG, Ladanyi M, Gerald 
            WL, Massague J
  TITLE     WNT/TCF signaling through LEF1 and HOXB9 mediates lung 
            adenocarcinoma metastasis.
  JOURNAL   Cell. 2009 Jul 10. 138(1):51-62.
  PUBMED    19576624
REFERENCE   2  (bases 1 to 540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 10. 138(1):51-62."
COMMENT     Sequence Label: MSCV-PM mir shLEF1 b
FEATURES             Location/Qualifiers
     source          1..540
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        198..446
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     198..218
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     369..388
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 caccatggcc cctcgctcca aaaatgcttt cgcgtcgcgc agacactgct cggtagtttc
       61 ggggatcagc gtttgagtaa gagcccgcgt ctgaaccctc cgcgccgccc cggccccagt
      121 ggaaagacgc gcaggcaaaa cgcaccacgt gacggagcgt gaccgcgcgc cgagcgcgcg
      181 ccagggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
      241 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
      301 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
      361 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
      421 atatcttgtg gaaaggacga aacaccgtgc tcgcttcggc agcacatata ctagtcgact
      481 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
//
