LOCUS       sequence-33252-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Nguyen DX, Chiang AC, Zhang XH, Kim JY, Kris MG, Ladanyi M, Gerald 
            WL, Massague J
  TITLE     WNT/TCF signaling through LEF1 and HOXB9 mediates lung 
            adenocarcinoma metastasis.
  JOURNAL   Cell. 2009 Jul 10. 138(1):51-62.
  PUBMED    19576624
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 10. 138(1):51-62."
COMMENT     Sequence Label: MSCV-PM mir shLEF1 a
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           257..378
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           406..420
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           448..574
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(623..646)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgtgctcgc ttcggcagca catatactag tcgactaggg
      241 ataacagggt aattgtttga atgaggcttc agtactttac agaatcgttg cctgcacatc
      301 ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct cgagaaggta
      361 tattgctgtt gacagtgagc gccgttgctg agtgtactct aaatagtgaa gccacagatg
      421 tatttagagt acactcagca acgatgccta ctgcctcgga attcaagggg ctactttagg
      481 agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa
      541 agctgaatta aaatggtata aattaaatca cttttttcaa ttctaccggg taggggaggc
      601 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      661 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
      721 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
      781 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
      841 cgtgcagatg gacagcaccg ct
//
