LOCUS       sequence-332524-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti virus delivery of AsCpf1 and crRNA guide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, 
            DeGennaro EM, Winblad N, Choudhury SR, Abudayyeh OO, Gootenberg JS, 
            Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F
  TITLE     Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.
  JOURNAL   Nat Biotechnol. 2017 Jan;35(1):31-34. doi: 10.1038/nbt.3737. Epub 
            2016 Dec 5.
  PUBMED    27918548
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3737"; 
            journalName: "Nat Biotechnol"; date: "2017-01"; volume: "35"; issue:
            "1"; pages: "31-34"
COMMENT     Sequence Label: pY108 (lenti-AsCpf1)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     108..127
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    279..396
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(694..738)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
       61 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
      121 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
      181 acaccgtaat ttctactctt gtagatggag acgatatatc gtctcttttt taattaaatt
      241 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
      301 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
      361 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
      421 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc
      481 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
      541 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
      601 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
      661 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
      721 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
      781 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
      841 ttcca
//
