LOCUS       sequence-332529-        6890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  injections to make Bmp reporter mouse transgenic line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Ivanovitch K, Soro-Barrio P, Chakravarty P, Jones RA, Bell DM, 
            Mousavy Gharavy SN, Stamataki D, Delile J, Smith JC, Briscoe J
  TITLE     Ventricular, atrial, and outflow tract heart progenitors arise from 
            spatially and molecularly distinct regions of the primitive streak.
  JOURNAL   PLoS Biol. 2021 May 17;19(5):e3001200. doi: 
            10.1371/journal.pbio.3001200. eCollection 2021 May.
  PUBMED    33999917
REFERENCE   2  (bases 1 to 6890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2021-05-17"; volume: "19(5):e3001200. doi"; pages: " 
            10.1371/journal.pbio.3001200. eCollection 2021 May"
COMMENT     Sequence Label: pJC5-4-insulator-BREreporter-insulator
FEATURES             Location/Qualifiers
     source          1..6890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..42)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(25..41)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(34..56)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(250..269)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     369..391
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(429..447)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        515..619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(620..688,689..1480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(838..857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1651..2239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2140..2159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2393..2410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2527..2548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      4168..4177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4180..4557
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4570..4579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4576..4578,4579..4581,4582..5295)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2010)"
                     /label=mTurquoise
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4621..4642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4882..4901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5229..5250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5335..5456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5346..5365)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5400..5419
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tatagtgtca cctaaatcca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
       61 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      121 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      181 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
      241 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      301 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      361 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      421 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      481 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1141 cgagcgtgac accacgatgc ctgtagcaat gccaacaacg ttgcgcaaac tattaactgg
     1201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     1801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2221 ggggcggagc ctatcgaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2401 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2461 gattcattaa tgcagctggc acgacaggtt tcccgactcg aaagcgggca gtgagcgcaa
     2521 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacagc agcttataat
     2581 ggntacaaat aaagcaanag catcacaaat ttnnnnaata aagcaggaaa ccactacatt
     2641 ctagttgtgg tcaatccana atcagcatca tattagggca tcaatggagc acccggtcag
     2701 gtggactcta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     2761 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     2821 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     2881 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     2941 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     3001 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     3061 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     3121 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     3181 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     3241 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     3301 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     3361 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     3421 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     3481 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     3541 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggantgggac
     3601 aggcagctgg agnattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     3661 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     3721 cntcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     3781 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     3841 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     3901 tcagtgagga tggggctcta gggatccgca agtgcaggtg ccagaacatt tctctatcga
     3961 taggtaccga gctcttacgc gtgctagctc agaccgttag acgccaggac gggctgtcag
     4021 gctggcgccg cggcgccagc ctgacagccc gtcctggcgt ctaacggtct gagctagccc
     4081 gggctcgaga tctgggctat aaaagggggt gggggcgcgt tcgtcctcac tctcttccaa
     4141 gcttggcatt ccggtactgt tggtaaagcc accatggtta tgccagagcc agcgaagtct
     4201 gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc
     4261 aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag
     4321 caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg
     4381 aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc
     4441 tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg
     4501 gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaaggat
     4561 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4741 gtgccctggc ccaccctcgt gaccaccctg tcctggggcg tgcagtgctt cgcccgctac
     4801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4981 aacatcctgg ggcacaagct ggagtacaac tacatcagcg acaacgtcta tatcaccgcc
     5041 gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc
     5101 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5161 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     5221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5281 gagctgtaca agtaatctag agtcggggcg gccggccgct tcgagcagac atgataagat
     5341 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     5401 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     5461 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     5521 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     5581 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     5641 cggatccccg ggtaccgagc tcgctagagg gacagccccc ccccaaagcc cccagggatg
     5701 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     5761 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     5821 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     5881 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     5941 aacggcctgg gttgcaaagg agcacagtgc tcatccagat ccaaccccct gctatgtgca
     6001 gggtcatcaa ccagcagccc aggctgccca gagccacatc cagcctggcc ttgaatgcct
     6061 gcagggatgg ggcatccaca gcctccttgg gcaacctgtt cagtgcgtca ccaccctctg
     6121 ggggaaaaac tgcctcctca tatccaaccc aaacctcccc tgtctcagtg taaagccatt
     6181 cccccttgtc ctatcaaggg ggagtttgct gtgacattgt tggtctgggg tgacacatgt
     6241 ttgccaattc agtgcatcac ggagaggcag atcttgggga taaggaagtg caggacagca
     6301 tggacgtggg acatgcaggt gttgagggct ctgggacact ctccaagtca cagcgttcag
     6361 aacagcctta aggataagaa gataggatag aaggacaaag agcaagttaa aacccagcat
     6421 ggagaggagc acaaaaaggc cacagacact gctggtccct gtgtctgagc ctgcatgttt
     6481 gatggtgtct ggatgcaagc agaaggggtg gaagagcttg cctggagaga tacagctggg
     6541 tcagtaggac tgggacaggc agctggagaa ttgccatgta gatgttcata caatcgtcaa
     6601 atcatgaagg ctggaaaagc cctccaagat ccccaagacc aaccccaacc cacccaccgt
     6661 gcccactggc catgtccctc agtgccacat ccccacagtt cttcatcacc tccagggacg
     6721 gtgacccccc cacctccgtg ggcagctgtg ccactgcagc accgctcttt ggagaaggta
     6781 aatcttgcta aatccagccc gaccctcccc tggcacaacg taaggccatt atctctcatc
     6841 caactccagg acggagtcag tgaggatggg gctctagcga gctcgtattc
//
