LOCUS       sequence-332589-        4966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the synthetic gene BLADE FP6 under the constitutive 
            J23101** promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Romano E, Baumschlager A, Akmeric EB, Palanisamy N, Houmani M, 
            Schmidt G, Ozturk MA, Ernst L, Khammash M, Di Ventura B
  TITLE     Engineering AraC to make it responsive to light instead of 
            arabinose.
  JOURNAL   Nat Chem Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6.
  PUBMED    33903769
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6."
COMMENT     Sequence Label: pBLADE_ONLY_C
FEATURES             Location/Qualifiers
     source          1..4966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(52..711)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     626..645
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(712..814)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1206..1223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1340..1885)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1420..1439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1961..1980
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     terminator      3234..3320
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3412..3439
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3458..3549
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4007..4462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4094..4113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4304..4325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
       61 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      121 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      181 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      241 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
      301 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      361 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      421 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      481 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      541 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      601 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      661 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      721 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      781 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
      841 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
      901 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta
      961 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     1021 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc
     1081 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     1141 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     1201 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     1261 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     1321 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     1381 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1441 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     1501 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     1561 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
     1621 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
     1681 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
     1741 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
     1801 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
     1861 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta
     1921 ggctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     1981 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2041 tctgctcatg tttgacagct tatcattttt ttaggctacc gatgtagcct aacgcgatcg
     2101 atttatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
     2161 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
     2221 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
     2281 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     2341 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     2401 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     2461 ggtggatgga gcgactcgtt ttccgtttcg cactggaaac ccatgctgta ccggtattcc
     2521 cctgtttcat ctcgcaccgg aatcatcgtc aagaagttga caaaccgttg gccgttcttc
     2581 ttaaaattga ccacctcaac ctgcacctcg gcgttcctat caatcgcttt cctcatcgta
     2641 ttgatcgtgt tggagtcgac gtacttcctt gtcgatttcg gcttgaccat tccgtcgggt
     2701 gactgaagaa aacggcagtt tctccccaag acctccgcat tgctgtatcc tgtcatatag
     2761 agaaaagctt ccgaggcgta cacaattggc gtgtcttttt gcttcaggtc gcacagaatc
     2821 agagcaactg acgtgtcaac aggtcccagt tctacttggg ggtttggcct cttcataatc
     2881 tgaatcagat agcccataat gtcataaccg ccgggagcgt agagcgtatg catggtatat
     2941 ctccttctct agtctctagt agctagcata tacctaggac tgagctagtg taaactctag
     3001 aaatgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc
     3061 cgagctcgcg ccgattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg
     3121 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag
     3181 cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa
     3241 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     3301 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg
     3361 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca
     3421 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat
     3481 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3541 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3601 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3661 ttgggtgcag caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     3721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     3781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     3841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     3901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttacgc gccctgtagc
     4021 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4081 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4141 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4201 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     4261 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4321 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     4381 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     4441 aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     4501 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     4561 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4621 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4681 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     4741 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4801 accagtcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta
     4861 aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg
     4921 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttat
//
