LOCUS       sequence-332594-        6128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the synthetic gene BLADE FP6 under the constitutive 
            J23101** promoter. mCherry expression is controlled by the PBAD 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Romano E, Baumschlager A, Akmeric EB, Palanisamy N, Houmani M, 
            Schmidt G, Ozturk MA, Ernst L, Khammash M, Di Ventura B
  TITLE     Engineering AraC to make it responsive to light instead of 
            arabinose.
  JOURNAL   Nat Chem Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6.
  PUBMED    33903769
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2021 Apr 26. pii: 10.1038/s41589-021-00787-6. doi: 
            10.1038/s41589-021-00787-6."
COMMENT     Sequence Label: pBLADE(FP6**)-mCherry
FEATURES             Location/Qualifiers
     source          1..6128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(713..1372)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1287..1306
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1373..1475)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1867..1884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2001..2546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2081..2100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2622..2641
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        3799..4083
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4006..4025
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4138..4848
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4283..4301)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4551..4570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4887..4904)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5057..5143
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5235..5262
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5281..5372
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(5830..6128,1..157)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5917..5936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(6127..6128,1..20)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
       61 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      121 aacgcgaatt ttaacaaaat attaacgttt acaatttaaa aggatctagg tgaagatcct
      181 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      241 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      301 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      361 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
      421 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      481 tctgctaatc ctgttaccag tcaggcattt gagaagcaca cggtcacact gcttccggta
      541 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
      601 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
      661 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
      721 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      781 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      841 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      901 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      961 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1021 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1081 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1141 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1201 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1261 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1321 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1381 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1441 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     1501 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     1561 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttactgattt
     1621 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
     1681 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctag
     1741 cggagtgtat actggcttac tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg
     1801 tggcaggaga aaaaaggctg caccggtgcg tcagcagaat atgtgataca ggatatattc
     1861 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc
     1921 ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca gggaagtgag
     1981 agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaatc
     2041 tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2101 cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2161 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2221 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     2281 taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg cagcagccac
     2341 tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc taaactgaaa
     2401 ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa gagttggtag
     2461 ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca gagcaagaga
     2521 ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata aaatatttct
     2581 aggctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
     2641 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
     2701 atctgctcat gtttgacagc ttatcatttt tttaggctac cgatgtagcc taacgcgatc
     2761 gatttatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
     2821 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
     2881 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
     2941 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
     3001 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
     3061 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat
     3121 cggtggatgg agcgactcgt tttccgtttc gcactggaaa cccatgctgt accggtattc
     3181 ccctgtttca tctcgcaccg gaatcatcgt caagaagttg acaaaccgtt ggccgttctt
     3241 cttaaaattg accacctcaa cctgcacctc ggcgttccta tcaatcgctt tcctcatcgt
     3301 attgatcgtg ttggagtcga cgtacttcct tgtcgatttc ggcttgacca ttccgtcggg
     3361 tgactgaaga aaacggcagt ttctccccaa gacctccgca ttgctgtatc ctgtcatata
     3421 gagaaaagct tccgaggcgt acacaattgg cgtgtctttt tgcttcaggt cgcacagaat
     3481 cagagcaact gacgtgtcaa caggtcccag ttctacttgg gggtttggcc tcttcataat
     3541 ctgaatcaga tagcccataa tgtcataacc gccgggagcg tagagcgtat gcatggtata
     3601 tctccttctc tagtctctag tagctagcat atacctagga ctgagctagt gtaaactcta
     3661 gaaatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact
     3721 ccgtcaagcc gtcaattgtc tgattcgtta ccaacggatg cgccatgaca ggacttttca
     3781 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
     3841 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
     3901 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
     3961 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
     4021 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
     4081 catacccgtt tttttgggct agcgaattcg agctcggtac ctaaggagga tttaaagatg
     4141 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     4201 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4261 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     4321 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     4381 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4441 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctcctt gcaggacggc
     4501 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     4561 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     4621 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     4681 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     4741 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     4801 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaaa gcttggctgt
     4861 tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     4921 ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg
     4981 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta
     5041 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     5101 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     5161 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     5221 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     5281 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5341 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5401 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5461 aagtaaaaga tgctgaagat cagttgggtg cagcaaacta ttaactggcg aactacttac
     5521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5641 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5761 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5821 gattgattta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5881 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5941 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6001 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     6061 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6121 ttaatagt
//
