LOCUS       sequence-33260-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pQCXIH-Flag Smad3 EPSM
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..133
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
       61 tggaacggac ctgcaggaat tgatccgcgg ccgcaccggt gtgaccatgg actacaagga
      121 cgacgatgac aagtcgtcca tcctgccttt cactcccccg atcgtgaagc gcctgctggg
      181 ctggaagaag ggcgagcaga acgggcagga ggagaaatgg tgcgagaagg cggtcaagag
      241 cctggtcaag aaactcaaga agacggggca gctggacgag ctggagaagg ccatcaccac
      301 gcagaacgtc aacaccaagt gcatcaccat ccccaggtcc ctggatggcc ggttgcaggt
      361 gtcccatcgg aaggggctcc ctcatgtcat ctactgccgc ctgtggcgat ggccagacct
      421 gcacagccac cacgagctgc gggccatgga gctgtgtgag ttcgccttca atatgaagaa
      481 ggacgaggtg tgcgtcaatc cctaccacta ccagagagta gagacaccag ttctacctcc
      541 tgtgttggtg ccacgccaca cagagatccc ggccgagttc cccccactgg acgactacag
      601 ccattccatc cccgaaaaca ctaacttccc cgcaggcatc gagccccaga gcaatattcc
      661 agaggtccct ccccctggct acctgagtga agatggagaa accagtgacc accagatgaa
      721 ccacagcatg gacgcaggtg ctccaaacct agccccgaat ccgatggccc cagcacataa
      781 taacttggac ctgcagccag ttacctactg cgaagccggc ccttctggtg ctccatctcc
      841 tactacga
//
