LOCUS       sequence-332601-        7390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Purification of catalytically-dead DPP9S from insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pFastBac HTB DPP9S D808N
FEATURES             Location/Qualifiers
     source          1..7390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(345..350,351..367,368..373))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        725..816
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     741..758
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             883..900
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             922..942
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3709..3843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3746..3765)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3800..3819
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3872..4037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4221..4675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4308..4327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4517..4538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4701..4805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4806..4874,4875..5666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5024..5043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5837..6425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6326..6345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6590..6607
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6741..6965
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(7032..7390,1..175))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gggagtaggt ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg
       61 atttgacttg gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta
      121 actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac
      181 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
      241 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
      301 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
      361 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
      421 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
      481 cggtctccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
      541 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
      601 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
      661 tgttcgccca ggactctagc tatagttcta gtggttggct acgtatactc cggaatatta
      721 atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt attttactgt
      781 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
      841 accatcgggc gcggatctcg gtccgaaacc atgtcgtact accatcacca tcaccatcac
      901 gattacgata tcccaacgac cgaaaacctg tattttcagg gcgccatggg atccatggcc
      961 accaccggga ccccaacggc cgaccgaggc gacgcagccg ccacagatga cccggccgcc
     1021 cgcttccagg tgcagaagca ctcgtgggac gggctccgga gcatcatcca cggcagccgc
     1081 aagtactcgg gcctcattgt caacaaggcg ccccacgact tccagtttgt gcagaagacg
     1141 gatgagtctg ggccccactc ccaccgcctc tactacctgg gaatgccata tggcagccga
     1201 gagaactccc tcctctactc tgagattccc aagaaggtcc ggaaagaggc tctgctgctc
     1261 ctgtcctgga agcagatgct ggatcatttc caggccacgc cccaccatgg ggtctactct
     1321 cgggaggagg agctgctgag ggagcggaaa cgcctggggg tcttcggcat cacctcctac
     1381 gacttccaca gcgagagtgg cctcttcctc ttccaggcca gcaacagcct cttccactgc
     1441 cgcgacggcg gcaagaacgg cttcatggtg tcccctatga aaccgctgga aatcaagacc
     1501 cagtgctcag ggccccggat ggaccccaaa atctgccctg ccgaccctgc cttcttctcc
     1561 ttcatcaata acagcgacct gtgggtggcc aacatcgaga caggcgagga gcggcggctg
     1621 accttctgcc accaaggttt atccaatgtc ctggatgacc ccaagtctgc gggtgtggcc
     1681 accttcgtca tacaggaaga gttcgaccgc ttcactgggt actggtggtg ccccacagcc
     1741 tcctgggaag gttcagaggg cctcaagacg ctgcgaatcc tgtatgagga agtcgatgag
     1801 tccgaggtgg aggtcattca cgtcccctct cctgcgctag aagaaaggaa gacggactcg
     1861 tatcggtacc ccaggacagg cagcaagaat cccaagattg ccttgaaact ggctgagttc
     1921 cagactgaca gccagggcaa gatcgtctcg acccaggaga aggagctggt gcagcccttc
     1981 agctcgctgt tcccgaaggt ggagtacatc gccagggccg ggtggacccg ggatggcaaa
     2041 tacgcctggg ccatgttcct ggaccggccc cagcagtggc tccagctcgt cctcctcccc
     2101 ccggccctgt tcatcccgag cacagagaat gaggagcagc ggctagcctc tgccagagct
     2161 gtccccagga atgtccagcc gtatgtggtg tacgaggagg tcaccaacgt ctggatcaat
     2221 gttcatgaca tcttctatcc cttcccccaa tcagagggag aggacgagct ctgctttctc
     2281 cgcgccaatg aatgcaagac cggcttctgc catttgtaca aagtcaccgc cgttttaaaa
     2341 tcccagggct acgattggag tgagcccttc agccccgggg aagatgaatt taagtgcccc
     2401 attaaggaag agattgctct gaccagcggt gaatgggagg ttttggcgag gcacggctcc
     2461 aagatctggg tcaatgagga gaccaagctg gtgtacttcc agggcaccaa ggacacgccg
     2521 ctggagcacc acctctacgt ggtcagctat gaggcggccg gcgagatcgt acgcctcacc
     2581 acgcccggct tctcccatag ctgctccatg agccagaact tcgacatgtt cgtcagccac
     2641 tacagcagcg tgagcacgcc gccctgcgtg cacgtctaca agctgagcgg ccccgacgac
     2701 gaccccctgc acaagcagcc ccgcttctgg gctagcatga tggaggcagc cagctgcccc
     2761 ccggattatg ttcctccaga gatcttccat ttccacacgc gctcggatgt gcggctctac
     2821 ggcatgatct acaagcccca cgccttgcag ccagggaaga agcaccccac cgtcctcttt
     2881 gtatatggag gcccccaggt gcagctggtg aataactcct tcaaaggcat caagtacttg
     2941 cggctcaaca cactggcctc cctgggctac gccgtggttg tgattgacgg caggggctcc
     3001 tgtcagcgag ggcttcggtt cgaaggggcc ctgaaaaacc aaatgggcca ggtggagatc
     3061 gaggaccagg tggagggcct gcagttcgtg gccgagaagt atggcttcat cgacctgagc
     3121 cgagttgcca tccatggctg gtcctacggg ggcttcctct cgctcatggg gctaatccac
     3181 aagccccagg tgttcaaggt ggccatcgcg ggtgccccgg tcaccgtctg gatggcctac
     3241 gacacagggt acactgagcg ctacatggac gtccctgaga acaaccagca cggctatgag
     3301 gcgggttccg tggccctgca cgtggagaag ctgcccaatg agcccaaccg cttgcttatc
     3361 ctccacggct tcctgaacga aaacgtgcac tttttccaca caaacttcct cgtctcccaa
     3421 ctgatccgag cagggaaacc ttaccagctc cagatctacc ccaacgagag acacagtatt
     3481 cgctgccccg agtcgggcga gcactatgaa gtcacgttgc tgcactttct acaggaatac
     3541 ctctagtcta gagcctgcag tctcgaggca tgcggtacca agcttgtcga gaagtactag
     3601 aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     3661 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     3721 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3781 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3841 atctgatcac tgatatcgcc taggagatcc gaaccagata agtgaaatct agttccaaac
     3901 tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt
     3961 ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac
     4021 tattttgtcc gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct
     4081 gccaactcca tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa
     4141 tttttctgtc atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg
     4201 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4261 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4321 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4381 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4441 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4501 gtccacgttc ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4561 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4621 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4801 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5041 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5341 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctg
     6481 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6541 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6601 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     6661 ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg agtaataaat
     6721 ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa caaaatagat
     6781 ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga aatcagtcca
     6841 gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact cttcattttc
     6901 tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg gtaaagacta
     6961 tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc gatctcggct
     7021 tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt
     7081 atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag
     7141 atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag cgcggtggca
     7201 atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat ctcactacgc
     7261 ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg
     7321 aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta atcggagtcc
     7381 ggctgatgtt
//
