LOCUS       sequence-332604-       13558 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) 
            with N-terminal c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13558)
  AUTHORS   Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, 
            Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, 
            Rehman J, Malik AB
  TITLE     Engineered ACE2 decoy mitigates lung injury and death induced by 
            SARS-CoV-2 variants.
  JOURNAL   Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 
            10.1038/s41589-021-00965-6. Epub 2022 Jan 19.
  PUBMED    35046611
REFERENCE   2  (bases 1 to 13558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00965-6"; journalName: "Nat Chem Biol"; date: 
            "2022-03"; volume: "18"; issue: "3"; pages: "342-351"
COMMENT     Sequence Label: pCEP4-myc-S (Wuhan variant)
FEATURES             Location/Qualifiers
     source          1..13558
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1303..1321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1389..1493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1494..1562,1563..2354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1712..1731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2525..3113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3014..3033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3544..3689
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3732..4769
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4767..4786)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4811..4859
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        5513..5892
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5893..6096
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6047..6067
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6093..6117
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6199..6228
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    10043..10177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10067..10086)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10121..10140
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(10320..10342)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10931..12720
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc
       61 tcctgggtca tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc
      121 tgggccacct tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg
      181 ccttctacct ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg
      241 actcctgggc ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc
      301 ccccctggct ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc
      361 tccactacct cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc
      421 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc
      481 tgcccctcct gcccctcctg ctcctgcccc ttcctgcccc tcctgcccct cctcctgctc
      541 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc
      601 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
      661 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
      721 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
      781 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
      841 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
      901 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
      961 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
     1021 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
     1081 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
     1141 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
     1201 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
     1261 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
     1321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt
     3181 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag
     3241 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt
     3301 agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat
     3361 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc
     3421 tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat ccccgtggcc
     3481 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
     3541 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
     3601 tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
     3661 accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg
     3721 ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     3781 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     3841 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     3901 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     3961 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     4021 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     4081 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     4141 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     4201 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     4261 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     4321 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     4381 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     4441 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     4501 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     4561 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     4621 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     4681 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     4741 cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     4801 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     4861 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     4921 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     4981 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg gccccgtggg
     5041 ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat tattttgggc
     5101 gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt tttggatggc
     5161 ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac
     5221 acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag tcgaccaatt
     5281 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     5341 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     5401 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     5461 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     5521 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5581 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5641 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5701 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5761 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5821 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     5881 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     5941 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6001 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     6061 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
     6121 agaagctggg taccagctgc tagcgccacc atgaagacca tcatcgccct gagctacatc
     6181 ttctgcctgg tgttcgccga gcaaaagctg atctcagagg aggacctggg cagccctggc
     6241 ggtgccgtca atctgacaac tcggactcag ctgccacctg cttatactaa tagcttcacc
     6301 agaggcgtgt actatcctga caaggtgttt agaagctccg tgctgcactc tacacaggat
     6361 ctgtttctgc cattctttag caacgtgacc tggttccacg ccatccacgt gagcggcacc
     6421 aatggcacaa agcggttcga caatcccgtg ctgcctttta acgatggcgt gtacttcgcc
     6481 tctaccgaga agagcaacat catcagaggc tggatctttg gcaccacact ggactccaag
     6541 acacagtctc tgctgatcgt gaacaatgcc accaacgtgg tcatcaaggt gtgcgagttc
     6601 cagttttgta atgatccctt cctgggcgtg tactatcaca agaacaataa gagctggatg
     6661 gagtccgagt ttagagtgta ttctagcgcc aacaactgca catttgagta cgtgagccag
     6721 cctttcctga tggacctgga gggcaagcag ggcaatttca agaacctgag ggagttcgtg
     6781 tttaagaata tcgacggcta cttcaaaatc tactctaagc acacccccat caacctggtg
     6841 cgcgacctgc ctcagggctt cagcgccctg gagcccctgg tggatctgcc tatcggcatc
     6901 aacatcaccc ggtttcagac actgctggcc ctgcacagaa gctacctgac acccggcgac
     6961 tcctctagcg gatggaccgc cggcgctgcc gcctactatg tgggctacct ccagccccgg
     7021 accttcctgc tgaagtacaa cgagaatggc accatcacag acgcagtgga ttgcgccctg
     7081 gaccccctga gcgagacaaa gtgtacactg aagtccttta ccgtggagaa gggcatctat
     7141 cagacatcca atttcagggt gcagccaacc gagtctatcg tgcgctttcc taatatcaca
     7201 aacctgtgcc catttggcga ggtgttcaac gcaacccgct tcgccagcgt gtacgcctgg
     7261 aataggaagc ggatcagcaa ctgcgtggcc gactatagcg tgctgtacaa ctccgcctct
     7321 ttcagcacct ttaagtgcta tggcgtgtcc cccacaaagc tgaatgacct gtgctttacc
     7381 aacgtctacg ccgattcttt cgtgatcagg ggcgacgagg tgcgccagat cgcccccggc
     7441 cagacaggca agatcgcaga ctacaattat aagctgccag acgatttcac cggctgcgtg
     7501 atcgcctgga acagcaacaa tctggattcc aaagtgggcg gcaactacaa ttatctgtac
     7561 cggctgttta gaaagagcaa tctgaagccc ttcgagaggg acatctctac agaaatctac
     7621 caggccggca gcaccccttg caatggcgtg gagggcttta actgttattt cccactccag
     7681 tcctacggct tccagcccac aaacggcgtg ggctatcagc cttaccgcgt ggtggtgctg
     7741 agctttgagc tgctgcacgc cccagcaaca gtgtgcggcc ccaagaagtc caccaatctg
     7801 gtgaagaaca agtgcgtgaa cttcaacttc aacggcctga ccggcacagg cgtgctgacc
     7861 gagtccaaca agaagttcct gccatttcag cagttcggca gggacatcgc agataccaca
     7921 gacgccgtgc gcgacccaca gaccctggag atcctggaca tcacaccctg ctctttcggc
     7981 ggcgtgagcg tgatcacacc cggcaccaat acaagcaacc aggtggccgt gctgtatcag
     8041 gacgtgaatt gtaccgaggt gcccgtggct atccacgccg atcagctgac cccaacatgg
     8101 cgggtgtaca gcaccggctc caacgtcttc cagacaagag ccggatgcct gatcggagca
     8161 gagcacgtga acaattccta tgagtgcgac atcccaatcg gcgccggcat ctgtgcctct
     8221 taccagaccc agacaaactc tcccagaaga gcccggagcg tggcctccca gtctatcatc
     8281 gcctatacca tgtccctggg cgccgagaac agcgtggcct actctaacaa tagcatcgcc
     8341 atcccaacca acttcacaat ctctgtgacc acagagatcc tgcccgtgtc catgaccaag
     8401 acatctgtgg actgcacaat gtatatctgt ggcgattcta ccgagtgcag caacctgctg
     8461 ctccagtacg gcagcttttg tacccagctg aatagagccc tgacaggcat cgccgtggag
     8521 caggataaga acacacagga ggtgttcgcc caggtgaagc aaatctacaa gaccccccct
     8581 atcaaggact ttggcggctt caatttttcc cagatcctgc ctgatccatc caagccttct
     8641 aagcggagct ttatcgagga cctgctgttc aacaaggtga ccctggccga tgccggcttc
     8701 atcaagcagt atggcgattg cctgggcgac atcgcagcca gggacctgat ctgcgcccag
     8761 aagtttaatg gcctgaccgt gctgccaccc ctgctgacag atgagatgat cgcacagtac
     8821 acaagcgccc tgctggccgg caccatcaca tccggatgga ccttcggcgc aggagccgcc
     8881 ctccagatcc cctttgccat gcagatggcc tataggttca acggcatcgg cgtgacccag
     8941 aatgtgctgt acgagaacca gaagctgatc gccaatcagt ttaactccgc catcggcaag
     9001 atccaggaca gcctgtcctc tacagccagc gccctgggca agctccagga tgtggtgaat
     9061 cagaacgccc aggccctgaa taccctggtg aagcagctga gcagcaactt cggcgccatc
     9121 tctagcgtgc tgaatgacat cctgagccgg ctggacaagg tggaggcaga ggtgcagatc
     9181 gaccggctga tcaccggccg gctccagagc ctccagacct atgtgacaca gcagctgatc
     9241 agggccgccg agatcagggc cagcgccaat ctggcagcaa ccaagatgtc cgagtgcgtg
     9301 ctgggccagt ctaagagagt ggacttttgt ggcaagggct atcacctgat gtccttccct
     9361 cagtctgccc cacacggcgt ggtgtttctg cacgtgacct acgtgcccgc ccaggagaag
     9421 aacttcacca cagcccctgc catctgccac gatggcaagg cccactttcc aagggagggc
     9481 gtgttcgtgt ccaacggcac ccactggttt gtgacacagc gcaatttcta cgagccccag
     9541 atcatcacca cagacaacac cttcgtgagc ggcaactgtg acgtggtcat cggcatcgtg
     9601 aacaataccg tgtatgatcc actccagccc gagctggaca gctttaagga ggagctggat
     9661 aagtatttca agaatcacac ctcccctgac gtggatctgg gcgacatcag cggcatcaat
     9721 gcctccgtgg tgaacatcca gaaggagatc gaccgcctga acgaggtggc taagaatctg
     9781 aacgagagcc tgatcgacct ccaggagctg ggcaagtatg agcagtacat caagtggccc
     9841 tggtacatct ggctgggctt catcgccggc ctgatcgcca tcgtgatggt gaccatcatg
     9901 ctgtgctgta tgacatcctg ctgttcttgc ctgaagggct gctgtagctg tggctcctgc
     9961 tgtaagtttg acgaggatga ctctgaacct gtgctgaagg gcgtgaagct gcattacacc
    10021 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
    10081 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
    10141 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
    10201 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
    10261 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    10321 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    10381 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
    10441 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
    10501 ccmwyctwms ckgrsyaskr catstmwkcc ccttcttcgc ggggcagtgc atgtaatccc
    10561 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
    10621 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
    10681 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
    10741 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
    10801 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
    10861 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
    10921 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
    10981 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
    11041 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
    11101 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
    11161 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
    11221 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
    11281 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
    11341 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
    11401 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
    11461 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
    11521 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
    11581 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
    11641 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
    11701 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
    11761 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
    11821 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
    11881 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
    11941 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
    12001 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
    12061 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
    12121 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
    12181 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
    12241 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
    12301 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
    12361 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
    12421 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
    12481 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
    12541 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
    12601 gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg gagtgctatc
    12661 ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg
    12721 ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg
    12781 attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag
    12841 ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga cgaaaatggt
    12901 gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg gggatttatt
    12961 ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct aacaagttac
    13021 atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg tcatctccgt
    13081 catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc aaatctactc
    13141 catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc tttgtcataa
    13201 caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt aaaaagacca
    13261 tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt ccaggggcca
    13321 ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca agggcagttc
    13381 ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca ccggcgaccc
    13441 aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct cttaaacctt
    13501 ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt ccaaacca
//
