LOCUS       sequence-332609-       14050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / 
            Brazilian variant) with N-terminal c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Zhang L, Dutta S, Xiong S, Chan M, Chan KK, Fan TM, Bailey KL, 
            Lindeblad M, Cooper LM, Rong L, Gugliuzza AF, Shukla D, Procko E, 
            Rehman J, Malik AB
  TITLE     Engineered ACE2 decoy mitigates lung injury and death induced by 
            SARS-CoV-2 variants.
  JOURNAL   Nat Chem Biol. 2022 Mar;18(3):342-351. doi: 
            10.1038/s41589-021-00965-6. Epub 2022 Jan 19.
  PUBMED    35046611
REFERENCE   2  (bases 1 to 14050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00965-6"; journalName: "Nat Chem Biol"; date: 
            "2022-03"; volume: "18"; issue: "3"; pages: "342-351"
COMMENT     Sequence Label: pCEP4-myc-S (P.1 lineage / Brazilian variant)
FEATURES             Location/Qualifiers
     source          1..14050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        289..668
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        669..872
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     823..843
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             975..1004
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4819..4953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4843..4862)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4897..4916
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5096..5118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      5678..7467
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(7769..9694)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGXGXGGGXGXGGGXGGXGGXGXGGGXGXGGGAGGXGXGGGX
                     GGXGGXGXGGGXGXGGGGGGGGXGGGXGGXGGXGXGGGAGXGGXGGAGGAGAGGAGXGG
                     GAGGAGGXGAGGAGAGGAGAGGAGAGGAGGAGXGGXGGXGXGGXGGXGXGGGXGGXGXG
                     GGXGGXGXGGXGGXGXGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(10129..10147)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10215..10319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10320..10388,10389..11180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10538..10557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11351..11939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11840..11859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        12370..12515
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             12558..13595
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(13593..13612)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    13637..13685
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
       61 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
      121 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
      181 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      241 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat
      301 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      481 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      541 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      601 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      661 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
      721 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      781 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
      841 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atctctagaa
      901 gctgggtacc agctgctagc gccaccatga agaccatcat cgccctgagc tacatcttct
      961 gcctggtgtt cgccgagcaa aagctgatct cagaggagga cctgggcagc cctggcggtg
     1021 ccgtcaattt cacaaatcgg actcagctgc catctgctta tactaatagc ttcaccagag
     1081 gcgtgtacta tcctgacaag gtgtttagaa gctccgtgct gcactctaca caggatctgt
     1141 ttctgccatt ctttagcaac gtgacctggt tccacgccat ccacgtgagc ggcaccaatg
     1201 gcacaaagcg gttcgacaat cccgtgctgc cttttaacga tggcgtgtac ttcgcctcta
     1261 ccgagaagag caacatcatc agaggctgga tctttggcac cacactggac tccaagacac
     1321 agtctctgct gatcgtgaac aatgccacca acgtggtcat caaggtgtgc gagttccagt
     1381 tttgtaatta tcccttcctg ggcgtgtact atcacaagaa caataagagc tggatggagt
     1441 ccgagtttag agtgtattct agcgccaaca actgcacatt tgagtacgtg agccagcctt
     1501 tcctgatgga cctggagggc aagcagggca atttcaagaa cctgagcgag ttcgtgttta
     1561 agaatatcga cggctacttc aaaatctact ctaagcacac ccccatcaac ctggtgcgcg
     1621 acctgcctca gggcttcagc gccctggagc ccctggtgga tctgcctatc ggcatcaaca
     1681 tcacccggtt tcagacactg ctggccctgc acagaagcta cctgacaccc ggcgactcct
     1741 ctagcggatg gaccgccggc gctgccgcct actatgtggg ctacctccag ccccggacct
     1801 tcctgctgaa gtacaacgag aatggcacca tcacagacgc agtggattgc gccctggacc
     1861 ccctgagcga gacaaagtgt acactgaagt cctttaccgt ggagaagggc atctatcaga
     1921 catccaattt cagggtgcag ccaaccgagt ctatcgtgcg ctttcctaat atcacaaacc
     1981 tgtgcccatt tggcgaggtg ttcaacgcaa cccgcttcgc cagcgtgtac gcctggaata
     2041 ggaagcggat cagcaactgc gtggccgact atagcgtgct gtacaactcc gcctctttca
     2101 gcacctttaa gtgctatggc gtgtccccca caaagctgaa tgacctgtgc tttaccaacg
     2161 tctacgccga ttctttcgtg atcaggggcg acgaggtgcg ccagatcgcc cccggccaga
     2221 caggcacgat cgcagactac aattataagc tgccagacga tttcaccggc tgcgtgatcg
     2281 cctggaacag caacaatctg gattccaaag tgggcggcaa ctacaattat ctgtaccggc
     2341 tgtttagaaa gagcaatctg aagcccttcg agagggacat ctctacagaa atctaccagg
     2401 ccggcagcac cccttgcaat ggcgtgaagg gctttaactg ttatttccca ctccagtcct
     2461 acggcttcca gcccacatac ggcgtgggct atcagcctta ccgcgtggtg gtgctgagct
     2521 ttgagctgct gcacgcccca gcaacagtgt gcggccccaa gaagtccacc aatctggtga
     2581 agaacaagtg cgtgaacttc aacttcaacg gcctgaccgg cacaggcgtg ctgaccgagt
     2641 ccaacaagaa gttcctgcca tttcagcagt tcggcaggga catcgcagat accacagacg
     2701 ccgtgcgcga cccacagacc ctggagatcc tggacatcac accctgctct ttcggcggcg
     2761 tgagcgtgat cacacccggc accaatacaa gcaaccaggt ggccgtgctg tatcagggcg
     2821 tgaattgtac cgaggtgccc gtggctatcc acgccgatca gctgacccca acatggcggg
     2881 tgtacagcac cggctccaac gtcttccaga caagagccgg atgcctgatc ggagcagagt
     2941 acgtgaacaa ttcctatgag tgcgacatcc caatcggcgc cggcatctgt gcctcttacc
     3001 agacccagac aaactctccc agaagagccc ggagcgtggc ctcccagtct atcatcgcct
     3061 ataccatgtc cctgggcgcc gagaacagcg tggcctactc taacaatagc atcgccatcc
     3121 caaccaactt cacaatctct gtgaccacag agatcctgcc cgtgtccatg accaagacat
     3181 ctgtggactg cacaatgtat atctgtggcg attctaccga gtgcagcaac ctgctgctcc
     3241 agtacggcag cttttgtacc cagctgaata gagccctgac aggcatcgcc gtggagcagg
     3301 ataagaacac acaggaggtg ttcgcccagg tgaagcaaat ctacaagacc ccccctatca
     3361 aggactttgg cggcttcaat ttttcccaga tcctgcctga tccatccaag ccttctaagc
     3421 ggagctttat cgaggacctg ctgttcaaca aggtgaccct ggccgatgcc ggcttcatca
     3481 agcagtatgg cgattgcctg ggcgacatcg cagccaggga cctgatctgc gcccagaagt
     3541 ttaatggcct gaccgtgctg ccacccctgc tgacagatga gatgatcgca cagtacacaa
     3601 gcgccctgct ggccggcacc atcacatccg gatggacctt cggcgcagga gccgccctcc
     3661 agatcccctt tgccatgcag atggcctata ggttcaacgg catcggcgtg acccagaatg
     3721 tgctgtacga gaaccagaag ctgatcgcca atcagtttaa ctccgccatc ggcaagatcc
     3781 aggacagcct gtcctctaca gccagcgccc tgggcaagct ccaggatgtg gtgaatcaga
     3841 acgcccaggc cctgaatacc ctggtgaagc agctgagcag caacttcggc gccatctcta
     3901 gcgtgctgaa tgacatcctg agccggctgg acaaggtgga ggcagaggtg cagatcgacc
     3961 ggctgatcac cggccggctc cagagcctcc agacctatgt gacacagcag ctgatcaggg
     4021 ccgccgagat cagggccagc gccaatctgg cagcaatcaa gatgtccgag tgcgtgctgg
     4081 gccagtctaa gagagtggac ttttgtggca agggctatca cctgatgtcc ttccctcagt
     4141 ctgccccaca cggcgtggtg tttctgcacg tgacctacgt gcccgcccag gagaagaact
     4201 tcaccacagc ccctgccatc tgccacgatg gcaaggccca ctttccaagg gagggcgtgt
     4261 tcgtgtccaa cggcacccac tggtttgtga cacagcgcaa tttctacgag ccccagatca
     4321 tcaccacaga caacaccttc gtgagcggca actgtgacgt ggtcatcggc atcgtgaaca
     4381 ataccgtgta tgatccactc cagcccgagc tggacagctt taaggaggag ctggataagt
     4441 atttcaagaa tcacacctcc cctgacgtgg atctgggcga catcagcggc atcaatgcct
     4501 ccgtggtgaa catccagaag gagatcgacc gcctgaacga ggtggctaag aatctgaacg
     4561 agagcctgat cgacctccag gagctgggca agtatgagca gtacatcaag tggccctggt
     4621 acatctggct gggcttcatc gccggcctga tcgccatcgt gatggtgacc atcatgctgt
     4681 gctgtatgac atcctgctgt tcttgcctga agggctgctg tagctgtggc tcctgctgta
     4741 agtttgacga ggatgactct gaacctgtgc tgaagggcgt gaagctgcat tacacctaac
     4801 tcgaggccgg caaggccgga tccagacatg ataagataca ttgatgagtt tggacaaacc
     4861 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4921 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4981 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5041 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     5101 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     5161 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga
     5221 ctctagagga tcgatccccg ccccggacga actaaacctg actacgacat ctctgcccct
     5281 tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc
     5341 ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga
     5401 catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga
     5461 ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct
     5521 cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta
     5581 caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat
     5641 tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg
     5701 gtagtagtat atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa
     5761 gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc
     5821 ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat
     5881 tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt
     5941 cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt
     6001 aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg
     6061 gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt
     6121 gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca
     6181 tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta tggctatggg
     6241 caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga
     6301 ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg
     6361 ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca
     6421 cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg
     6481 ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta
     6541 ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc
     6601 actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg
     6661 attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt
     6721 gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag
     6781 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     6841 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     6901 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     6961 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     7021 tgctatccta atagagatta gggtagtata tgctatccta atttatatct gggtagcata
     7081 tactacccaa atatctggat agcatatgct atcctaatct atatctgggt agcatatgct
     7141 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     7201 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     7261 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     7321 atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat
     7381 acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt
     7441 gaattttcgc tgcttgtcct tttcctgctg gttgctccca ttcttaggtg aatttaagga
     7501 ggccaggcta aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc taatgttttc
     7561 caacgcgaga aggtgttgag cgcggagctg agtgacgtga caacatgggt atgcccaatt
     7621 gccccatgtt gggaggacga aaatggtgac aagacagatg gccagaaata caccaacagc
     7681 acgcatgatg tctactgggg atttattctt tagtgcgggg gaatacacgg cttttaatac
     7741 gattgagggc gtctcctaac aagttacatc actcctgccc ttcctcaccc tcatctccat
     7801 cacctccttc atctccgtca tctccgtcat caccctccgc ggcagcccct tccaccatag
     7861 gtggaaacca gggaggcaaa tctactccat cgtcaaagct gcacacagtc accctgatat
     7921 tgcaggtagg agcgggcttt gtcataacaa ggtccttaat cgcatccttc aaaacctcag
     7981 caaatatatg agtttgtaaa aagaccatga aataacagac aatggactcc cttagcgggc
     8041 caggttgtgg gccgggtcca ggggccattc caaaggggag acgactcaat ggtgtaagac
     8101 gacattgtgg aatagcaagg gcagttcctc gccttaggtt gtaaagggag gtcttactac
     8161 ctccatatac gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac
     8221 tcctagccag gagagctctt aaaccttctg caatgttctc aaatttcggg ttggaacctc
     8281 cttgaccacg atgctttcca aaccaccctc cttttttgcg cctgcctcca tcaccctgac
     8341 cccggggtcc agtgcttggg ccttctcctg ggtcatctgc ggggccctgc tctatcgctc
     8401 ccgggggcac gtcaggctca ccatctgggc caccttcttg gtggtattca aaataatcgg
     8461 cttcccctac agggtggaaa aatggccttc tacctggagg gggcctgcgc ggtggagacc
     8521 cggatgatga tgactgacta ctgggactcc tgggcctctt ttctccacgt ccacgacctc
     8581 tccccctggc tctttcacga cttccccccc tggctctttc acgtcctcta ccccggcggc
     8641 ctccactacc tcctcgaccc cggcctccac tacctcctcg accccggcct ccactgcctc
     8701 ctcgaccccg gcctccacct cctgctcctg cccctcctgc tcctgcccct cctcctgctc
     8761 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctsctcct scccctccts
     8821 cccctcctsc tcctscccct cctscccctc ctcctsctcc tscccctcct scccctcctc
     8881 ctsctcctsc ccctcctscc cctcctsctc ctscccctcc tscccctcct sctcctgccc
     8941 ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg
     9001 ctcctscccc tcctgcccct cctgcccctc ctcctsctcc tgcccctcct gctcctgccc
     9061 ctcctgcccc tcctscccct cctsctcctg cccctcctcc tsctcctscc cctcctsccc
     9121 ctcctscccc tcctcctsct cctccccctc ctccccctcc tcctsctcct scccctcctc
     9181 ctsctcctsc ccctcctscc cctcctsccc ctcctcctsc tcctscccct cctgcccctc
     9241 ctcctsctcc tscccctcct cctsctccts cccctcctsc ccctcctscc cctcctccts
     9301 ctcctscccc tcctcctsct cctscccctc ctgcccctcc tgcccctcct gcccctcctc
     9361 ctgctcctgc ccctcctcct gctcctgccc ctcctgctcc tgcccctccc gctcctgctc
     9421 ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt ttttggggtc
     9481 tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct cctggtcttc
     9541 cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc cctcttcttt
     9601 gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct cctaggccat
     9661 ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga gatcaataga
     9721 catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc ccctccaaca
     9781 gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc
     9841 ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga aaactcccgc
     9901 tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc
     9961 tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc ttcaaggtca
    10021 ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag
    10081 cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac
    10141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
    10201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
    10261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
    10321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
    10381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
    10441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
    10501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
    10561 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
    10621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
    10681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
    10741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
    10801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
    10861 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
    10921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    11041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    11101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    11161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    11221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    11281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    11341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    11401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    11461 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    11521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    11581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    11641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    11701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    11761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    11821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    11881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    11941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct
    12001 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc
    12061 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca
    12121 agacgtagcc cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg agatggcgga
    12181 cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg
    12241 attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccctg cttcatcccc
    12301 gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt
    12361 agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
    12421 atgcagtcgg ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc
    12481 tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc
    12541 ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
    12601 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
    12661 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
    12721 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
    12781 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
    12841 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
    12901 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
    12961 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
    13021 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
    13081 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
    13141 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
    13201 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
    13261 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
    13321 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
    13381 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
    13441 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
    13501 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
    13561 ctcgtccgga tcgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa
    13621 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
    13681 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    13741 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    13801 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc
    13861 cgtgggttag ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt
    13921 ttgggcgttg cgtggggtca ggtccacgac tggactgagc agacagaccc atggtttttg
    13981 gatggcctgg gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg
    14041 ccaaacaccc
//
