LOCUS       sequence-33261-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pQCXIH-Flag Smad3 AAGY
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             133..156
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
       61 gcattggaac ggacctgcag gaattgatcc gcggccatct gcagaattcg ccctttaatg
      121 cggccgacca tggactacaa ggacgacgat gacaagtcgt ccatcctgcc tttcactccc
      181 ccgatcgtga agcgcctgct gggctggaag aagggcgagc agaacgggca ggaggagaaa
      241 tggtgcgaga aggcggtcaa gagcctggtc aagaaactca agaagacggg gcagctggac
      301 gagctggaga aggccatcac cacgcagaac gtcaacacca agtgcatcac catccccagg
      361 tccctggatg gccggttgca ggtgtcccat cggaaggggc tccctcatgt catctactgc
      421 cgcctgtggc gatggccaga cctgcacagc caccacgagc tgcgggccat ggagctgtgt
      481 gagttcgcct tcaatatgaa gaaggacgag gtgtgcgtca atccctacca ctaccagaga
      541 gtagagacac cagttctacc tcctgtgttg gtgccacgcc acacagagat cccggccgag
      601 ttccccccac tggacgacta cagccattcc atccccgaaa acactaactt ccccgcaggc
      661 atcgagcccc agagcaatat tccagagacc ccagccgctg gctacctgag tgaagatgga
      721 gaaaccagtg accaccagat gaaccacagc atggacgcag gttctccaaa cctatccccg
      781 aatccgatgt ccccagcaca taataacttg gacctgcagc cagttaccta ctgcgagccg
      841 gccttctggt gctccatctc ctactacgag ctgaaccagc gcgtcgggga gacattccac
      901 gcctcgcagc catccatgac tgtggatggc
//
