LOCUS       sequence-332612-       12311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD
FEATURES             Location/Qualifiers
     source          1..12311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..564
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(156..173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     383..402
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             576..971
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1142..1611
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1741..1758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1912..2500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1992..2011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2664..3455,3456..3524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3287..3306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3525..3629)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3659..4252
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4315..4672
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4731..5147
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5077..5099
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5115..5131
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5157..5531
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5469..5485
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5558..5567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12235..12258
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
       61 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      121 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      181 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      241 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      301 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      361 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
      421 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
      481 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatatggcca caaccatggt tcctttgtct caagaagaat
      601 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
      661 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
      721 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
      781 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
      841 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
      901 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
      961 gggagggcta agtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga
     1021 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     1081 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
     1141 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     1201 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     1261 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
     1321 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     1381 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     1441 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
     1501 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     1561 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc acatgcagca
     1621 tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc catagttgca
     1681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2581 atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat ctaaagtata
     2641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgcataa
     3661 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     3721 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
     3781 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     3841 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     3901 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     3961 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     4021 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     4081 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
     4141 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     4201 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     4261 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     4321 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     4381 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     4441 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     4501 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca cccccctaat
     4561 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     4621 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     4681 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag ggccagactg
     4741 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
     4801 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
     4861 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
     4921 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
     4981 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
     5041 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
     5101 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
     5161 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
     5221 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
     5281 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
     5341 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
     5401 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
     5461 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
     5521 cctctagact gccggatcta gctagttaat taaggatcca tggcggccct agtgttggag
     5581 gacgggtcgg tcctgcgggg ccagcccttt ggggccgccg tgtcgactgc cggggaagtg
     5641 gtgtttcaaa ccggcatggt cggctacccc gaggccctca ctgatccctc ctacaaggca
     5701 cagatcttag tgctcaccta tcctctgatc ggcaactatg gcatcccccc agatgaaatg
     5761 gatgagttcg gtctctgcaa gtggtttgaa tcctcgggca tccacgtagc agcactggta
     5821 gtgggagagt gctgtcctac tcccagccac tggagtgcca cccgcaccct gcatgagtgg
     5881 ctgcagcagc atggcatccc tggcttgcaa ggagtagaca ctcgggagct gaccaagaag
     5941 ttgcgggaac aggggtctct gctggggaag ctggtccaga atggaacaga accttcaagt
     6001 ctgccattct tggaccccaa tgcccgcccc ctggtaccag aggtctccat taagactcca
     6061 cgggtattca atacaggggg tgcccctcgg atccttgctt tggactgtgg cctcaagtat
     6121 aatcagatcc gatgcctctg ccagcgtggg gctgaggtca ctgtggtacc ctgggaccat
     6181 gcactagaca gccaagagta tgagggtctc ttcttaagta atgggcctgg tgaccctgcc
     6241 tcctatccca gtgtcgtatc cacactgagc cgtgttttat ctgagcctaa tccccgacct
     6301 gtctttggga tctgcctggg acaccagcta ttggccttag ccattggggc caagacttac
     6361 aagatgagat atgggaaccg aggccataac cagccctgct tgttggtggg ctctgggcgc
     6421 tgctttctga catcccagaa ccatgggttt gctgtggaga cagactcact gccagcagac
     6481 tgggctcctc tcttcaccaa cgccaatgat ggttccaatg aaggcattgt gcacaacagc
     6541 ttgcctttct tcagtgtcca gtttcaccca gagcaccaag ctggcccttc agatatggaa
     6601 ctgcttttcg atatctttct ggaaactgtg aaagaggcca cagctgggaa ccctgggggc
     6661 cagacagtta gagagcggct gactgagcgc ctctgtcccc ctgggattcc cactcccggc
     6721 tctggacttc caccaccacg aaaggttctg atcctgggct cagggggcct ctccattggc
     6781 caagctggag aatttgacta ctcgggctct caggcaatta aggccctgaa ggaggaaaac
     6841 atccagacgt tgctgatcaa ccccaatatt gccacagtgc agacctccca ggggctggcc
     6901 gacaaggtct attttcttcc cataacacct cattatgtaa cccaggtgat acgtaatgaa
     6961 cgccccgatg gtgtgttact gacttttggg ggccagactg ctctgaactg tggtgtggag
     7021 ctgaccaagg ccggggtgct ggctcggtat ggggtccggg tcctgggcac accagtggag
     7081 accattgagc tgaccgagga tcgacgggcc tttgctgcca gaatggcaga gatcggagag
     7141 catgtggccc cgagcgaggc agcaaattct cttgaacagg cccaggcagc cgctgaacgg
     7201 ctggggtacc ctgtgctagt gcgtgcagcc tttgccctgg gtggcctggg ctctggcttt
     7261 gcctctaaca gggaggagct ctctgctctc gtggccccag cttttgccca taccagccaa
     7321 gtgctagtag acaagtctct gaagggatgg aaggagattg agtacgaggt ggtgagagac
     7381 gcctatggca actgtgtcac ggtgtgtaac atggagaact tggacccact gggcatccac
     7441 actggtgagt ccatagtggt ggcccctagc cagacactga atgacaggga gtatcagctc
     7501 ctgaggcaga cagctatcaa ggtgacccag cacctgggaa ttgttgggga gtgcaatgtg
     7561 cagtatgcct tgaaccctga gtctgagcag tattacatca ttgaagtgaa tgccaggctc
     7621 tctcgcagct ctgccctggc cagtaaggcc acaggttatc cactggctta tgtggcagcc
     7681 aagctagcat tgggcatccc tttgcctgag ctcaggaact ctgtgacagg gggtacagca
     7741 gcctttgaac ccagcgtgga ttattgtgtg gtgaagattc ctcgatggga ccttagcaag
     7801 ttcctgcgag tcagcacaaa gattgggagc tgcatgaaga gcgttggtga agtcatgggc
     7861 attgggcgtt catttgagga ggccttccag aaggccctgc gcatggtgga tgagaactgt
     7921 gtgggctttg atcacacagt gaaaccagtc agcgatatgg agttggagac tccaacagat
     7981 aagcggattt ttgtggtggc agctgctttg tgggctggtt attcagtgga ccgcctgtat
     8041 gagctcacac gcatcgaccg ctggttcctg caccgaatga agcgtatcat cgcacatgcc
     8101 cagctgctag aacaacaccg tggacagcct ttgccgccag acctgctgca acaggccaag
     8161 tgtcttggct tctcagacaa acagattgcc cttgcagttc tgagcacaga gctggctgtt
     8221 cgcaagctgc gtcaggaact ggggatctgt ccagcagtga aacagattga cacagttgca
     8281 gctgagtggc cagcccagac aaattaccta tacctaacgt attggggcac cacccatgac
     8341 ctcacctttc gaacacctca tgtcctagtc cttggctctg gcgtctaccg tattggctct
     8401 agcgttgaat ttgactggtg tgctgtaggc tgcatccagc agctccgaaa gatgggatat
     8461 aagaccatca tggtgaacta taacccagag acagtcagca ccgactatga catgtgtgat
     8521 cgactctact ttgatgagat ctcttttgag gtggtgatgg acatctatga gctcgagaac
     8581 cctgaaggtg tgatcctatc catgggtgga cagctgccca acaacatggc catggcgttg
     8641 catcggcagc agtgccgggt gctgggcacc tcccctgaag ccattgactc ggctgagaac
     8701 cgtttcaagt tttcccggct ccttgacacc attggtatca gccagcctca gtggagggag
     8761 ctcagtgacc tcgagtctgc tcgccaattc tgccagaccg tggggtaccc ctgtgtggtg
     8821 cgcccctcct atgtgctgag cggtgctgct atgaatgtgg cctacacgga tggagacctg
     8881 gagcgcttcc tgagcagcgc agcagccgtc tccaaagagc atcccgtggt catctccaag
     8941 ttcatccagg aggctaagga gattgacgtg gatgccgtgg cctctgatgg tgtggtggca
     9001 gccatcgcca tctctgagca tgtggagaat gcaggtgtgc attcaggtga tgcgacgctg
     9061 gtgacccccc cacaagatat cactgccaaa accctggagc ggatcaaagc cattgtgcat
     9121 gctgtgggcc aggagctaca ggtcacagga cccttcaatc tgcagctcat tgccaaggat
     9181 gaccagctga aagttattga atgcaacgta cgtgtctctc gctccttccc cttcgtttcc
     9241 aagacactgg gtgtggacct agtagccttg gccacgcggg tcatcatggg ggaagaagtg
     9301 gaacctgtgg ggctaatgac tggttctgga gttgtgggag taaaggtgcc tcagttctcc
     9361 ttctcccgct tggcgggtgc tgacgtggtg ttgggtgtgg aaatgaccag tactggggag
     9421 gtggccggct ttggggagag ccgctgtgag gcatacctca aggccatgct aagcactggc
     9481 tttaagatcc ccaagaagaa tatcctgctg accattggca gctataagaa caaaagcgag
     9541 ctgctcccaa ctgtgcggct actggagagc ctgggctaca gcctctatgc cagtctcggc
     9601 acagctgact tctacactga gcatggcgtc aaggtaacag ctgtggactg gcactttgag
     9661 gaggctgtgg atggtgagtg cccaccacag cggagcatcc tggagcagct agctgagaaa
     9721 aactttgagc tggtgattaa cctgtcaatg cgtggagctg ggggccggcg tctctcttcc
     9781 tttgtcacca agggctaccg cacccgacgc ttggccgctg acttctccgt gcccctaatc
     9841 atcgatatca agtgcaccaa actctttgtg gaggccctag gccagatcgg gccagcccct
     9901 cctttgaagg tgcatgttga ctgtatgacc tcccaaaagc ttgtgcgact gccgggattg
     9961 attgatgtcc atgtgcacct gcgggaacca ggtgggacac ataaggagga ctttgcttca
    10021 ggcacagccg ctgccctggc tgggggtatc accatggtgt gtgccatgcc taatacccgg
    10081 ccccccatca ttgacgcccc tgctctggcc ctggcccaga agctggcaga ggctggcgcc
    10141 cggtgcgact ttgcgctatt ccttggggcc tcgtctgaaa atgcaggaac cttgggcacc
    10201 gtggccgggt ctgcagccgg gctgaagctt tacctcaatg agaccttctc tgagctgcgg
    10261 ctggacagcg tggtccagtg gatggagcat ttcgagacat ggccctccca cctccccatt
    10321 gtggctcacg cggagcagca aaccgtggct gctgtcctca tggtggctca gctcactcag
    10381 cgctcagtgc acatatgtca cgtggcacgg aaggaggaga tcctgctaat taaagctgca
    10441 aaggcacggg gcttgccagt gacctgcgag gtggctcccc accacctgtt cctaagccat
    10501 gatgacctgg agcgcctggg gcctgggaag ggggaggtcc ggcctgagct tggctcccgc
    10561 caggatgtgg aagccctgtg ggagaacatg gctgtcatcg actgctttgc ctcagaccat
    10621 gctccccata ccttggagga gaagtgtggg tccaggcccc cacctgggtt cccagggtta
    10681 gagaccatgc tgccactact cctgacggct gtaagcgagg gccggctcag cctggacgac
    10741 ctgctgcagc gattgcacca caatcctcgg cgcatctttc acctgcctcc gcaggaggac
    10801 acctatgtgg aggtggatct ggagcatgag tggacaattc ccagccacat gcccttctcc
    10861 aaggcccact ggacaccttt tgaagggcag aaagtgaagg gcaccgtccg ccgtgtggtc
    10921 ctgcgagggg aggttgccta tatcgatggg caggttctgg tacccccggg ctatggacag
    10981 gatgtacgga agtggccaca gggggctgtt cctcagctcc caccctcagc ccctgccact
    11041 agtgagatga ccacgacacc tgaaagaccc cgccgtggca tcccagggct tcctgatggc
    11101 cgcttccatc tgccgccccg aatccatcga gcctccgacc caggtttgcc agctgaggag
    11161 ccaaaggaga agtcctctcg gaaggtagcc gagccagagc tgatgggaac ccctgatggc
    11221 acctgctacc ctccaccacc agtaccgaga caggcatctc cccagaacct ggggacccct
    11281 ggcttgctgc acccccagac ctcacccctg ctgcactcat tagtgggcca acatatcctg
    11341 tccgtccagc agttcaccaa ggatcagatg tctcacctgt tcaatgtggc acacacactg
    11401 cgtatgatgg tgcagaagga gcggagcctc gacatcctga aggggaaggt catggcctcc
    11461 atgttctatg aagtgagcac acggaccagc agctcctttg cagcagccat ggcccggctg
    11521 ggaggtgctg tgctcagctt ctcggaagcc acatcgtccg tccagaaggg cgaatccctg
    11581 gctgactccg tgcagaccat gagctgctat gccgacgtcg tcgtgctccg gcacccccag
    11641 cctggagcag tggagctggc cgccaagcac tgccggaggc cagtgatcaa tgctggggat
    11701 ggggtcggag agcaccccac ccaggccctg ctggacatct tcaccatccg tgaggagctg
    11761 ggaactgtca atggcatgac gatcacgatg gtgggtgacc tgaagcacgg acgcacagta
    11821 cattccctgg cctgcctgct cacccagtat cgtgtcagcc tgcgctacgt ggcacctccc
    11881 agcctgcgca tgccacccac tgtgcgggcc ttcgtggcct cccgcggcac caagcaggag
    11941 gaattcgaga gcattgagga ggcgctgcct gacactgatg tgctctacat gactcgaatc
    12001 cagaaggaac gatttggctc tacccaggag tacgaagctt gctttggtca gttcatcctc
    12061 actccccaca tcatgacccg ggccaagaag aagatggtgg tgatgcaccc gatgccccgt
    12121 gtcaacgaga taagcgtgga agtggactcg gatccccgcg cagcctactt ccgccaggct
    12181 gagaacggca tgtacatccg catggctctg ttagccaccg tgctgggccg tttcgactac
    12241 aaagacgatg acgacaagta ggaattcctg caggcctcga gggccggcgc gccgcggccg
    12301 ctacgtaaat t
//
