LOCUS       sequence-332643-        2493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ethanol system inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , 
            Nakayama, Naomi
  TITLE     Mobius Assembly for Plant Systems highlights promoter-terminator 
            interaction in gene regulation
  JOURNAL   bioRxiv, March 31, 2021
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv, 
            March 31, 2021"
COMMENT     Sequence Label: lvl0 alcSynth-34S
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5..62
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(86..103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(257..845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(337..356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1028..1122)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1143..1802)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1717..1736
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1964..1982
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1986..2029)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2425..2480
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
       61 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      121 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      181 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      241 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac
      301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
      781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
      841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
      901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
      961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
     1021 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1081 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1141 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     1201 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1261 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1321 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     1381 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1441 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1501 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1561 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1621 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1681 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1741 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     1801 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     1861 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
     1921 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1981 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt
     2041 ctctggagga cccatgcgga gccgcattgg aggccatgcg gagccgcacg cgttaacaag
     2101 agcggctccg cttgacctct cgggatagtt ccgacctagg attggatgca tgcggaaccg
     2161 cacgagggcg gggcggaaat tgacacacca ctcctctcca cgcaccgttc aagaggtacg
     2221 cgtatagagc cgtatagagc agagacggag cactttctgg tactgtccgc acgggatgtc
     2281 cgcacggaga gccacaaacg agcggggccc cgtacgtgct ctcctacccc aggatcgcat
     2341 ccccgcatag ctgaacatct atataagaag gcattcattc ccatttgaag gatcatcaga
     2401 tactgaacca atattatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     2461 caattactat ttacaattac aatgtgagac cct
//
