LOCUS       sequence-332718-       11728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet/Dox-inducible mammalian expression of CARD8 that cannot bind 
            DPP9 due to a mutation at site I (E274R) with sgRNA binding site 
            mutations .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pInducer20 CARD8-HA PAM E274R
FEATURES             Location/Qualifiers
     source          1..11728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    57..75
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    99..117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    225..243
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    298..415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(708..752)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    937..1170
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1667..1792
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1839..2472
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2664..2683)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2733..2749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2733..2749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2746..2768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2757..2773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2781..2787,2788..2805,2806..2811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2826..2847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3087..3104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3258..3846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3338..3357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4017..4808,4809..4877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4640..4659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4878..4982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5050..5068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5187..5420
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5495..6083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5601..5612
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6010..6030
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6260..7054)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6260..7054)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6371..6390)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6981..7000
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    7070..7643
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7230..7249)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     7459..7476
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(7695..7703,7704..7739,7740..7742,7743..7778,
                     7779..7781,7782..7817,7818..7823,7824..8441))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(8500..9711)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(8644..8663)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(9797..9823)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    9876..9900
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11518..11542)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11651..11675)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(11672..11710)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc
       61 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
      121 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
      181 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
      241 ggagtggtaa aggatcctag tccaaactgg atctctgctg tccctgtaat aaacccgaaa
      301 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
      361 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
      421 gatgaatact gccatttgtg aattcggcga taccgtcgag atccgttcac taatcgaatg
      481 gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc
      541 ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc
      601 actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg
      661 aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat
      721 ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
      781 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
      841 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
      901 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
      961 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     1021 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     1081 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     1141 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     1201 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     1261 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     1321 ggccggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     1381 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     1441 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     1501 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     1561 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     1621 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     1681 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     1741 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     1801 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     1861 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1921 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1981 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     2041 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     2101 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     2161 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     2221 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     2281 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     2341 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     2401 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttctt tgggagtgaa
     2461 ttagcccttc caactactaa gtttgtagta catatttaac aaatacaatt tctttaaaat
     2521 gaaaataatt cagaggaatc acaggtttag agtaaatgaa accacaggta attggcagtg
     2581 gtaatagggt atggggtggg aagtttggga tgattttggt tagcttgagt tatccagttg
     2641 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2701 aaaaatgctt tatttgtgaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     2761 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2821 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2881 ggaaacctgt cgtgccacgg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     2941 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     3001 aattagtcag ccatgagctt gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4621 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5041 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatgatc tagccctttc
     5101 cttaattaac ccgggcctct cactctctga tattcatttc tttgcaagtt ataaatactg
     5161 aataataaga tgacatgaac tactactgct agagattttc cacactgact aaaagggtct
     5221 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     5281 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     5341 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     5401 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     5461 tgccttgtaa gtcattggtc ttaaaggatc tcaggcgggg aggcggccca aagggagatc
     5521 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
     5581 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
     5641 agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt ccccgacaac
     5701 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
     5761 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
     5821 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
     5881 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
     5941 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
     6001 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     6061 attttgtaat ccagaggttg attaggatct atcgcgataa gcttgatatc gaattgggag
     6121 ggactaaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     6181 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
     6241 cggccgcttt acttgtacat cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     6301 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     6361 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     6421 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     6481 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg
     6541 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     6601 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     6661 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     6721 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     6781 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     6841 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
     6901 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     6961 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     7021 gagaacctgc gtgcaatcca tcttgttcaa tcatggtgga ccggtaagct tatcatcgtg
     7081 tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt tttaacctcg
     7141 actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata caatggggta
     7201 ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat ttgactcttt
     7261 ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca ggtgtatctt
     7321 atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc cgctgcctgc
     7381 aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc cagaggaact gcttccttca
     7441 cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta ggaatgctcg
     7501 tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg
     7561 aaaataacat atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt
     7621 tagggggggg ggagggagag gggggggggg agaggggcgg aattcctcta gtgcggccgc
     7681 ggatccttac ttagttaccc ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca
     7741 ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa
     7801 tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat
     7861 atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca
     7921 attgcttgtc tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt
     7981 gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac
     8041 agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt
     8101 tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg
     8161 cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat
     8221 gactcgcctt ccagggggca gaagtgggta tgatgcctgt ccagcatctc gattggcagg
     8281 gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc
     8341 agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgacaccatt gagtaattcc
     8401 agagctccgt ttatgacttt gctcttgtcc agtctagaca tggtgaattc gactgcagga
     8461 ccggtacggg gtggagatcc gagctcggta ccaagcttcg tctaacaaaa aagccaaaaa
     8521 cggccagaat ttagcggaca atttactagt ctaacactga aaattacata ttgacccaaa
     8581 tgattacatt tcaaaaggtg cctaaaaaac ttcacaaaac acactcgcca accccgagcg
     8641 catagttcaa aaccggagct tcagctactt aagaagatag gtacataaaa ccgaccaaag
     8701 aaactgacgc ctcacttatc cctcccctca ccagaggtcc ggcgcctgtc gattcaggag
     8761 agcctaccct aggcccgaac cctgcgtcct gcgacggaga aaagcctacc gcacacctac
     8821 cggcaggtgg ccccaccctg cattataagc caacagaacg ggtgacgtca cgacacgacg
     8881 agggcgcgcg ctcccaaagg tacgggtgca ctgcccaacg gcaccgccat aactgccgcc
     8941 cccgcaacag acgacaaacc gagttctcca gtcagtgaca aacttcacgt cagggtcccc
     9001 agatggtgcc ccagcccatc tcacccgaat aagagctttc ccgcattagc gaaggcctca
     9061 agaccttggg ttcttgccgc ccaccatgcc ccccaccttg tttcaacgac ctcacagccc
     9121 gcctcacaag cgtcttccat tcaagactcg ggaacagccg ccattttgct gcgctccccc
     9181 caacccccag ttcagggcaa ccttgctcgc ggacccagac tacagccctt ggcggtctct
     9241 ccacacgctt ccgtcccacc gagcggcccg gcggccacga aagccccggc cagcccagca
     9301 gcccgctact caccaagtga cgatcacagc gatccacaaa caagaaccgc gacccaaatc
     9361 ccggctgcga cggaactagc tgtgccacac ccggcgcgtc cttatataat catcggcgtt
     9421 caccgcccca cggagatccc tccgcagaat cgccgagaag ggactacttt tcctcgcctg
     9481 ttccgctctc tggaaagaaa accagtgccc tagagtcacc caagtcccgt cctaaaatgt
     9541 ccttctgctg atactggggt tctaaggccg agtcttatga gcagcgggcc gctgtcctga
     9601 gcgtccgggc ggaaggatca ggacgctcgc tgcgcccttc gtctgacgtg gcagcgctcg
     9661 ccgtgaggag gggggcgccc gcgggaggcg ccaaaacccg gcgcggaggc cagatcttgg
     9721 gtgggttact ccagactgcc ttgggaaaag cgcctcccct acccggtaga atttctagtt
     9781 taattaatca ttactaagcg tagtctggga cgtcgtatgg gtattcgaac cgcgggccct
     9841 ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt
     9901 ccaaattctg ctgtctaaga taggacacga ggtaagggtc cctttcacta atgcttctga
     9961 agagcacgtc cagggccagg tcccctttct tctccaccat gctcagcaag gcctcattct
    10021 tgctctgccg tgtcttttcc tgctccacca gctccttctc attctcagta agaacctcat
    10081 tgtcttggag atcatcgagc acccctttca ggtcccccat cctggcttgg agttgccggt
    10141 ggttctcctt cacaaaggct gcacctgaga aaggaggagg ggctgatgca gctacaagct
    10201 ggagatccac tggcttcacc tcagtatccc acaccaaagt cccatgtctt ttttcagtaa
    10261 tctcaagttg aatgggttcc ttcatctgcc cagcatagaa ttttgagaag tgctgaattt
    10321 ctccagggct cctgtaggac aatttcaact ccttgggcat tactttcagg ttagcagaat
    10381 tagacacaat ataactggaa ccaaagttca ggggttccat tgggggcgaa gtctgcaggc
    10441 gcacaccatg gaagcgatct tcctcatcat ctatcgcctt tgttagcaag gcgtcgctgg
    10501 ggacaaggta caagtggaac ttaatatctt cggggtgggg gtgataatag atcaatgtgt
    10561 tggaagtgat ggggatggag aggcgagtcc cactggcgat ccgcagcagg atgcccatca
    10621 gagagaagct ggggctttcc aggacagcat agaaaggctc cacccgggct ggatgctcca
    10681 ggaccatccc tctattctta aaatgggcaa cgagaaacca ggagacgtcc acctcacctg
    10741 cttggaggga gatgaagtgg gggaggtgga tttcggcgac agcctcctct ggctctgcag
    10801 tgacatcaaa caaggggccg cccaccagcc actgttcatg gtgctgcagg tccagggcca
    10861 ggtgctgact ccagctgccg aaggcgatgg taactgtgac ctcatccctt accaggaagc
    10921 cgaggcctgt ggctgaccac agataccagc cagcagtggg gaaccaaacg ctgtatctgt
    10981 ttgtgctctt atcaatcaac tcaacatcca catttccttc aggccccaga aactgacgat
    11041 ttttataatc ttcttcgatc tcaaaacaga ctttagaagc ataagaggaa actatttgat
    11101 tctcttctga gcaaatgtct cctgaatctt gtccctctga agattcctgc tcttctgata
    11161 cactgggaat gtccccccca gatagttgac actcaggaac agcacggaac aataatggct
    11221 ctgcctctgt ctcatcatct tcttggaaaa aatgtgagat gtcacaaagg gtctcagaaa
    11281 cacagggtag ctccctgtat accgtatcat ttgtcacaca ggcctcagcc tgaaaaaaaa
    11341 ttccagtttt tgtgtattgc agttcccgta tgctattgtc aaccaacagt ttccgtgatc
    11401 cttgtagtct aatgagtttg gatgcatcta tgttcctact ggatccactg tcccgtctcg
    11461 gcagctcttc ctcactgctg ctactctttt ctggacactc ctttttttcc atggcgaagc
    11521 ctgctttttt gtacaaactt gttgatatct gcagaattcc accacactgg actagttcgg
    11581 ggccgcggag gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc
    11641 gtctccaggc gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta
    11701 cacgcctacc tcgacccggg taccgagc
//
