LOCUS       sequence-33272-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 LC AAGY
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          91..223
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     206..225
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        268..286
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             304..327
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttatcacagt taattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc
       61 tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa
      121 ggagaccaat agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca
      181 cctattggtc ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc
      241 aattacagct cttaaggcta gagtacttaa tacgactcac tataggctag cctcgagacc
      301 atggactata aggatgacga tgacaaagaa ttcacgcgtg gtacctctag accagttcta
      361 cctcctgtgt tggtgccacg ccacacagag atcccggccg agttcccccc actggacgac
      421 tacagccatt ccatccccga aaacactaac ttccccgcag gcatcgagcc ccagagcaat
      481 attccagaga ccccagccgc tggctacctg agtgaagatg gagaaaccag tgaccaccag
      541 atgaaccaca gcatggacgc aggttctcca aacctatccc cgaatccgat gtccccagca
      601 cataataact tggacctgca gccagttacc tactgcgagc cggccttctg gtgctccatc
      661 tcctactacg agctgaacca gcgcgtcggg gagacattcc acgcctcgca gccatccatg
      721 actgtggatg gcttcaccga cccctccaat tcggagcgct tctgcctagg gctgctctcc
      781 aatgtcaaca ggaatgcagc agtggagctg acacggagac acatcggaag aggcgtgcgg
      841 ctctactaca tc
//
