LOCUS       sequence-33273-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL WT
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(712..735)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(753..771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(814..833)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 agcggccgct tagtaggtaa ctggctgcag gtccaagtta ttatgtgctg gggacatcgg
       61 attcggggat aggtttggag aacctgcgtc catgctgtgg ttcatctggt ggtcactggt
      121 ttctccatct tcactcaggt agccaggggg tggggtctct ggaatattgc tctggggctc
      181 gatgcctgcg gggaagttag tgttttcggg gatggaatgg ctgtagtcgt ccagtggggg
      241 gaactcggcc gggatctctg tgtggcgtgg caccaacaca ggaggtagaa ctggtgtctc
      301 tactctctgg tagtggtagg gattcacgca gacctcgtcc ttcttcatat tgaaggcgaa
      361 ctcacacagc tccatggccc gcagctcgtg gtggctgtgc aggtctggcc atcgccacag
      421 gcggcagtag atgacatgag ggagcccctt ccgatgggac acctgcaacc ggccatccag
      481 ggacctgggg atggtgatgc acttggtgtt gacgttctgc gtggtgatgg ccttctccag
      541 ctcgtccagc tgccccgtct tcttgagttt cttgaccagg ctcttgaccg ccttctcgca
      601 ccatttctcc tcctgcccgt tctgctcgcc cttcttccag cccagcaggc gcttcacgat
      661 cgggggagtg aaaggcagga tggacgatct agaggtacca cgcgtgaatt ctttgtcatc
      721 gtcatcctta tagtccatgg tctcgaggct agcctatagt gagtcgtatt aagtactcta
      781 gccttaagag ctgtaattga actgggagtg gacacctgtg gagagaaagg caaagtggat
      841 gtcagtaaga ccaataggtg cc
//
