LOCUS       sequence-332733-       10247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can 
            clone desired gRNA using BsaI digestion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Chen V, Griffin ME, Maguin P, Varble A, Hang HC
  TITLE     RecT recombinase expression enables efficient gene editing in 
            Enterococcus.
  JOURNAL   Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 
            10.1128/AEM.00844-21.
  PUBMED    34232061
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 
            10.1128/AEM.00844-21."
COMMENT     Sequence Label: pCas9
FEATURES             Location/Qualifiers
     source          1..10247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(170..248)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             544..4650
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             544..4647
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4671..4802
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   4803..4838
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   4869..4904
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(5086..5102)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5086..5102)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     rep_origin      6392..6980
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6881..6900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga
       61 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc
      121 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg
      181 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga
      241 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
      301 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
      361 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
      421 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac
      481 tattattgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca
      541 aaaatggata agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg
      601 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
      661 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
      721 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
      781 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
      841 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
      901 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
      961 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
     1021 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
     1081 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
     1141 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
     1201 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
     1261 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
     1321 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
     1381 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
     1441 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
     1501 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
     1561 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
     1621 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
     1681 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
     1741 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
     1801 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
     1861 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
     1921 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
     1981 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
     2041 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
     2101 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
     2161 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
     2221 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
     2281 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
     2341 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
     2401 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
     2461 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
     2521 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
     2581 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
     2641 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
     2701 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
     2761 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
     2821 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
     2881 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
     2941 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
     3001 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
     3061 cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
     3121 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
     3181 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
     3241 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
     3301 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
     3361 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
     3421 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
     3481 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
     3541 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
     3601 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
     3661 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
     3721 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
     3781 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
     3841 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
     3901 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
     3961 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
     4021 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
     4081 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
     4141 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
     4201 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
     4261 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
     4321 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
     4381 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
     4441 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
     4501 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
     4561 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
     4621 attgatttga gtcagctagg aggtgactga agtatatttt agatgaagat tatttcttaa
     4681 taactaaaaa tatggtataa tactcttaat aaatgcagta atacaggggc ttttcaagac
     4741 tgaagtctag ctgagacaaa tagtgcgatt acgaaatttt ttagacaaaa atagtctacg
     4801 aggttttaga gctatgctgt tttgaatggt cccaaaactg agaccagtct cggaagctca
     4861 aaggtctcgt tttagagcta tgctgttttg aatggtccca aaacttcagc acactgagac
     4921 ttgttgagtt ccatgtttta gagctatgct gttttgaatg gactccattc aacattgccg
     4981 atgataactt gagaaagagg gttaatacca gcagtcggat accttcctat tctttctgtt
     5041 aaagcgtttt catgttataa taggcaaaag aagagtagtg tgatggatcc actagttcta
     5101 gaatcgatac gattttgaag tggcaacaga taaaaaaaag cagtttaaaa ttgttgctga
     5161 acttttaaaa caagcaaata caatcattgt cgcaacagat agcgacagag aaggcgaaaa
     5221 cattgcctgg tcgatcattc ataaagcaaa tgccttttct aaagataaaa cgtataaaag
     5281 actatggatc aatagtttag aaaaagatgt gatccgtagc ggttttcaaa atttgcgacc
     5341 aggaatgaat tactatccct tttatcaaga agcgcacaaa aagaaaaacg aaatgataca
     5401 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
     5461 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
     5521 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
     5581 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
     5641 gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt
     5701 gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa
     5761 cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat
     5821 aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag gttattggga
     5881 taagttagag ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg
     5941 gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa
     6001 atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct
     6061 ttctattatt ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa
     6121 ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt
     6181 accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat
     6241 gaactctatt caggaattgt cagataggcc taatgactgg cttttataat tctatgagtc
     6301 gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta
     6361 tactactgac agcttccaag gagctaaaga gttgagatcc tttttttctg cgcgtaatat
     6421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6961 gggggcggag cctatggaaa cctagcgctt agaatcgctt taggaaacac gatccagtcc
     7021 aataatcgtc gataaaaact tttgaaaaag gttggtgaaa ttacctactt ttggaataat
     7081 cacaaatcac aagtgattaa tcacaaatca caagtgatta atcacttgtt tattaagata
     7141 ttaaaagcta taatttaaat aaagcgtgaa ttttattaca caaaaagagg ggggagaaac
     7201 ttggaactag catttagaga aagcttaaaa aagatgagag gtaccaaatc aaaagaaaaa
     7261 ttctcccaag aattagaaat gagtagatca aattattcac gaatagaatc aggaaaatca
     7321 gatccaacca taaaaacact agaacaaatt gcaaagttaa ctaactcaac gctagtagtg
     7381 gatttaatcc caaatgagcc aacagaacca gaaccagaaa cagaatcaga acaagtaaca
     7441 ttggatttag aaatggaaga agaaaaaagc aatgacttcg tgtgaataat gcgcgaaatc
     7501 gttgcttatt ttttttaaaa gcggtatact agatataacg aaacaacgaa ctgaatagaa
     7561 acgaaaaaag agccatgaca catttataaa atgtttgacg acattttata aatgcatagc
     7621 ccgataagat tgccaaacca acgcttatca gttagtcaga tgaactcttc cctcgtaaga
     7681 agttatttaa ttaactttgt ttgaagacgg tatataaccg tactatcatt atatagggaa
     7741 atcagagagt tttcaagtat ctaagctact gaatttaaga attgttaagc aatcaatcgg
     7801 aaatcgtttg attgcttttt ttgtattcat ttatagaagg tggagtttgt atgaatcatg
     7861 atgaatgtaa aacttatata aaaaatagtt tattggagat aagaaaatta gcaaatatct
     7921 atacactaga aacgtttaag aaagagttag aaaagagaaa tatctactta gaaacaaaat
     7981 cagataagta tttttcttcg gagggggaag attatatata taagttaata gaaaataaca
     8041 aaataattta ttcgattagt ggaaaaaaat tgacttataa aggaaaaaaa tctttttcaa
     8101 aacatgcaat attgaaacag ttgaatgaaa aagcaaacca agttaattaa acaacctatt
     8161 ttataggatt tataggaaag gagaacagct gaatgaatat cccttttgtt gtagaaactg
     8221 tgcttcatga cggcttgtta aagtacaaat ttaaaaatag taaaattcgc tcaatcacta
     8281 ccaagccagg taaaagcaaa ggggctattt ttgcgtatcg ctcaaaatca agcatgattg
     8341 gcggtcgtgg tgttgttctg acttccgagg aagcgattca agaaaatcaa gatacattta
     8401 cacattggac acccaacgtt tatcgttatg gaacgtatgc agacgaaaac cgttcataca
     8461 cgaaaggaca ttctgaaaac aatttaagac aaatcaatac cttctttatt gattttgata
     8521 ttcacacggc aaaagaaact atttcagcaa gcgatatttt aacaaccgct attgatttag
     8581 gttttatgcc tactatgatt atcaaatctg ataaaggtta tcaagcatat tttgttttag
     8641 aaacgccagt ctatgtgact tcaaaatcag aatttaaatc tgtcaaagca gccaaaataa
     8701 tttcgcaaaa tatccgagaa tattttggaa agtctttgcc agttgatcta acgtgtaatc
     8761 attttggtat tgctcgcata ccaagaacgg acaatgtaga attttttgat cctaattacc
     8821 gttattcttt caaagaatgg caagattggt ctttcaaaca aacagataat aagggcttta
     8881 ctcgttcaag tctaacggtt ttaagcggta cagaaggcaa aaaacaagta gatgaaccct
     8941 ggtttaatct cttattgcac gaaacgaaat tttcaggaga aaagggttta atagggcgta
     9001 ataacgtcat gtttaccctc tctttagcct actttagttc aggctattca atcgaaacgt
     9061 gcgaatataa tatgtttgag tttaataatc gattagatca acccttagaa gaaaaagaag
     9121 taatcaaaat tgttagaagt gcctattcag aaaactatca aggggctaat agggaataca
     9181 ttaccattct ttgcaaagct tgggtatcaa gtgatttaac cagtaaagat ttatttgtcc
     9241 gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg tcaacgtgtt catttgtcag
     9301 aatggaaaga agatttaatg gcttatatta gcgaaaaaag cgatgtatac aagccttatt
     9361 tagtgacgac caaaaaagag attagagaag tgctaggcat tcctgaacgg acattagata
     9421 aattgctgaa ggtactgaag gcgaatcagg aaattttctt taagattaaa ccaggaagaa
     9481 atggtggcat tcaacttgct agtgttaaat cattgttgct atcgatcatt aaagtaaaaa
     9541 aagaagaaaa agaaagctat ataaaggcgc tgacaaattc ttttgactta gagcatacat
     9601 tcattcaaga gactttaaac aagctagcag aacgccctaa aacggacaca caactcgatt
     9661 tgtttagcta tgatacaggc tgaaaataaa acccgcacta tgccattaca tttatatcta
     9721 tgatacgtgt ttgttttttc tttgctgttt agcgaatgat tagcagaaat atacagagta
     9781 agattttaat taattattag ggggagaagg agagagtagc ccgaaaactt ttagttggct
     9841 tggactgaac gaagtgaggg aaaggctact aaaacgtcga ggggcagtga gagcgaagcg
     9901 aacacttgat tttttaattt tctatctttt ataggtcatt agagtatact tatttgtcct
     9961 ataaactatt tagcagcata atagatttat tgaataggtc atttaagttg agcatattag
    10021 aggaggaaaa tcttggagaa atatttgaag aacccgatta catggattgg attagttctt
    10081 gtggttacgt ggtttttaac taaaagtagt gaatttttga tttttggtgt gtgtgtcttg
    10141 ttgttagtat ttgctagtca aagtgattaa atagaattct catgtttgac agcttatcat
    10201 cggagctccg atgataagct gtcaaacatg agaattcccg gggatcc
//
