LOCUS       sequence-332737-        6137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, pUC origin, Tn7 recognition 
            sites, VanRAM repressor, PVanCC promoter, mRFP, Gentamicin 
            Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pIVR5
FEATURES             Location/Qualifiers
     source          1..6137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..28
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        998..1047
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             1092..1103
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1110..1787
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     mobile_element  1867..2032
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(2302..2890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2382..2401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3061..3852,3853..3921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3684..3703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3922..4026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4094..4112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4150..4172)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4272..4291
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4705..4721
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4706..4722
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4750..4844
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4947..5033
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    5079..5126
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(5256..5789)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5978..5983,5984..6000,6001..6006))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    6047..6094
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtga aacagacgaa gaatccatgg tcaatctgca
       61 cgaattgacc atgctgcacc cagcggtgcg cctgcgcttg ctgctgcttc aaaaactttt
      121 gcattacgaa ttgctgccag tgcatgatca cgcataatac gttctgcacc ttcggcatca
      181 ccacagctaa ctgcatccag aactgcctga tgctgacgat gtgctgccag cagatgttca
      241 tattcggcag acaggtccat cagatccagg gccagtgcac cggctgctgc aaacggttca
      301 aaaccattac gtgccagtgc gctttcaact gcaccattac ctgctgcgct aaccagggta
      361 tcatgaaatg cctgattata tgcggcataa cgatccagat cttcaccatt caggcgaccg
      421 gctgcaaaca gtgcttcacc ttctgcaatc agtacaacaa aacgtgcatg ggtttctgag
      481 gtcataccac gttctgccag acgacgtgct gcaaaacctt ccagaacacc acgaacttca
      541 attgcatcac gaatctgatc gctgctaaca ccacgggctg cataaccacg tgcacccaga
      601 cgaacaacca gaccttcttg ttccagtgaa cgcagtgcga tacgaaccgg catacggcta
      661 acacccagtg ctgctgcggt cggaatttct gcaatacgtt caccactttt gatttcaccg
      721 cttgcaatca ttttacgcag tgccatcata acacgctgac ccggtttaat acgaggcatg
      781 tccatgtcta tcctccttat ttacgttcgt tagtttaagc gggcgctatc atgccatacc
      841 gcgaaaggtt ttgcgccatt cgatggcgcg ccgccaatta ttgaaggcct ccctaacggg
      901 gggccttttt ttgtttctgg tctcccgctt aacgatcgtt ggctgattgg atccaattga
      961 cagctagctc agtcctaggt accattggat ccaatagctg tcaccggatg tgctttccgg
     1021 tctgatgagt ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa gaactgggaa
     1081 gagcgcagat taaagaggag aaatactaga tggcttcctc cgaagacgtt atcaaagagt
     1141 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag
     1201 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag
     1261 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag
     1321 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt
     1381 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact
     1441 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt
     1501 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt
     1561 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg
     1621 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg
     1681 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg
     1741 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt
     1801 gctagtgtag atcgcgcttt ccagtcggga aacctgtcgt ggtacctcgc gaaggccttg
     1861 caggccaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
     1921 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
     1981 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
     2041 tttttcttcc tgttatgttt gggcgctgca ttaatgaatc ggccaacgcg cggggagagg
     2101 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gacccgctgc gctcggtcgt
     2161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2701 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3361 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3661 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4021 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4081 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     4141 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4201 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4261 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4321 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     4381 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tagaggacca
     4441 gccgcgtaac ctggcaaaat cggttacggt tgagtaataa atggatgccc tgcgtaagcg
     4501 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     4561 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     4621 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     4681 tcgtattaaa gaggggcgtg gggttcgagg tcgacggtat cgataagcta gcttaattag
     4741 ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt
     4801 tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagc tagactgcga
     4861 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     4921 ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     4981 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     5041 taggagcttg cggcccggac gatcgaattg gggatcttga agtacctatt ccgaagttcc
     5101 tattctctag aaagtatagg aacttcagag cgcttttgaa gctgatgtgc ttaaaaactt
     5161 actcaatgga attctagatc gaattggccg cggcgttgtg acaatttacc gaacaactcc
     5221 gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata
     5281 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg
     5341 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg
     5401 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga
     5461 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga
     5521 gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc
     5581 aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac
     5641 tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta
     5701 catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc
     5761 tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     5821 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc
     5881 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     5941 ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta
     6001 tgtcaattcg atccattgct gttgacaaag ggaatcaggg gatcttgaag ttcctattcc
     6061 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaagc taattcgatc
     6121 atgcatgagc tcactag
//
