LOCUS       sequence-33274-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL WT
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          98..230
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     213..232
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        275..293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             311..334
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa cagtctcgaa
       61 cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg ttacaagaca
      121 ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg
      181 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtccactc
      241 ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat aggctagcct
      301 cgagaccatg gactataagg atgacgatga caaagaattc acgcgtggta cctctagatc
      361 gtccatcctg cctttcactc ccccgatcgt gaagcgcctg ctgggctgga agaagggcga
      421 gcagaacggg caggaggaga aatggtgcga gaaggcggtc aagagcctgg tcaagaaact
      481 caagaagacg gggcagctgg acgagctgga gaaggccatc accacgcaga acgtcaacac
      541 caagtgcatc accatcccca ggtccctgga tggccggttg caggtgtccc atcggaaggg
      601 gctccctcat gtcatctact gccgcctgtg gcgatggcca gacctgcaca gccaccacga
      661 gctgcgggcc atggagctgt gtgagttcgc cttcaatatg aagaaggacg aggtctgcgt
      721 gaatccctac cactaccaga gagtagagac accagttcta cctcctgtgt tggtgccacg
      781 ccacacagag atcccggccg agttcccccc actggacgac tacagccatt ccatccccga
      841 aaacactaac ttccccgcag gcatc
//
