LOCUS       sequence-332745-        6226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, pBBR origin, VanRAM repressor, 
            PVanCC promoter, mRFP, Gentamicin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pBVR5
FEATURES             Location/Qualifiers
     source          1..6226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1217..1988
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1989..2651
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     misc_RNA        4345..4394
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             4439..4450
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4457..5134
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        join(5411..5416,5417..5433,5434..5439)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5628..6161
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct gggcctgttt
       61 ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct tgatgatcgc
      121 ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa cgggcttcag
      181 gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc gcatctcacg
      241 cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt ttgtcagccg
      301 gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt cggccaatcc
      361 ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt ggcccactgg
      421 tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct tgctgccctc
      481 gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct ggtcgcgggt
      541 catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct gcgtcagcgg
      601 caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg ccgtcacgat
      661 gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga acgccgcctg
      721 ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt actcgaccgc
      781 caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc ccatcgcttc
      841 atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt cagcgttggg
      901 cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca ttttcgccag
      961 cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt ggtgtccaac
     1021 cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg cttgagtata
     1081 ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc gccctacggg
     1141 cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccatcc cgtggatatg
     1201 tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt ggacaaccct
     1261 gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag ggattgccca
     1321 ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc tgcggccttg
     1381 ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc ctgcgcgctt
     1441 cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg accgcgcagc
     1501 ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc agtcgaaggg
     1561 cgaagcccgc ccgcctgccc cccgagcctc acggcggcga gtgcgggggt tccaaggggg
     1621 cagcgccacc ttgggcaagg ccgaaggccg cgcagtcgat caacaagccc cggaggggcc
     1681 actttttgcc ggagggggag ccgcgccgaa ggcgtggggg aaccccgcag gggtgccctt
     1741 ctttgggcac caaagaacta gatatagggc gaaatgcgaa agacttaaaa atcaacaact
     1801 taaaaaaggg gggtacgcaa cagctcattg cggcaccccc cgcaatagct cattgcgtag
     1861 gttaaagaaa atctgtaatt gactgccact tttacgcaac gcataattgt tgtcgcgctg
     1921 ccgaaaagtt gcagctgatt gcgcatggtg ccgcaaccgt gcggcacccc taccgcatgg
     1981 agataagcat ggccacgcag tccagagaaa tcggcattca agccaagaac aagcccggtc
     2041 actgggtgca aacggaacgc aaagcgcatg aggcgtgggc cgggcttatt gcgaggaaac
     2101 ccacggcggc aatgctgctg catcacctcg tggcgcagat gggccaccag aacgccgtgg
     2161 tggtcagcca gaagacactt tccaagctca tcggacgttc tttgcggacg gtccaatacg
     2221 cagtcaagga cttggtggcc gagcgctgga tctccgtcgt gaagctcaac ggccccggca
     2281 ccgtgtcggc ctacgtggtc aatgaccgcg tggcgtgggg ccagccccgc gaccagttgc
     2341 gcctgtcggt gttcagtgcc gccgtggtgg ttgatcacga cgaccaggac gaatcgctgt
     2401 tggggcatgg cgacctgcgc cgcatcccga ccctgtatcc gggcgagcag caactaccga
     2461 ccggccccgg cgaggagccg cccagccagc ccggcattcc gggcatggaa ccagacctgc
     2521 cagccttgac cgaaacggag gaatgggaac ggcgcgggca gcagcgcctg ccgatgcccg
     2581 atgagccgtg ttttctggac gatggcgagc cgttggagcc gccgacacgg gtcacgctgc
     2641 cgcgccggta gcacttgggt tgcgcagcaa cccgtaagtg cgctgttcca gactatcggc
     2701 tgtagccgcc tcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     2761 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccgttt
     2821 ttatcaggct ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga
     2881 gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag
     2941 tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac
     3001 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     3061 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     3121 gccctgccac tcatcgcagt acggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3181 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
     3241 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     3301 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     3361 taaacgacgg ccagtgaaac agacgaagaa tccatggtca atctgcacga attgaccatg
     3421 ctgcacccag cggtgcgcct gcgcttgctg ctgcttcaaa aacttttgca ttacgaattg
     3481 ctgccagtgc atgatcacgc ataatacgtt ctgcaccttc ggcatcacca cagctaactg
     3541 catccagaac tgcctgatgc tgacgatgtg ctgccagcag atgttcatat tcggcagaca
     3601 ggtccatcag atccagggcc agtgcaccgg ctgctgcaaa cggttcaaaa ccattacgtg
     3661 ccagtgcgct ttcaactgca ccattacctg ctgcgctaac cagggtatca tgaaatgcct
     3721 gattatatgc ggcataacga tccagatctt caccattcag gcgaccggct gcaaacagtg
     3781 cttcaccttc tgcaatcagt acaacaaaac gtgcatgggt ttctgaggtc ataccacgtt
     3841 ctgccagacg acgtgctgca aaaccttcca gaacaccacg aacttcaatt gcatcacgaa
     3901 tctgatcgct gctaacacca cgggctgcat aaccacgtgc acccagacga acaaccagac
     3961 cttcttgttc cagtgaacgc agtgcgatac gaaccggcat acggctaaca cccagtgctg
     4021 ctgcggtcgg aatttctgca atacgttcac cacttttgat ttcaccgctt gcaatcattt
     4081 tacgcagtgc catcataaca cgctgacccg gtttaatacg aggcatgtcc atgtctatcc
     4141 tccttattta cgttcgttag tttaagcggg cgctatcatg ccataccgcg aaaggttttg
     4201 cgccattcga tggcgcgccg ccaattattg aaggcctccc taacgggggg cctttttttg
     4261 tttctggtct cccgcttaac gatcgttggc tgattggatc caattgacag ctagctcagt
     4321 cctaggtacc attggatcca atagctgtca ccggatgtgc tttccggtct gatgagtccg
     4381 tgaggacgaa acagcctcta caaataattt tgtttaagaa ctgggaagag cgcagattaa
     4441 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa
     4501 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg
     4561 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc
     4621 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca
     4681 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg
     4741 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga
     4801 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt
     4861 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg
     4921 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc
     4981 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac
     5041 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga
     5101 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc
     5161 gcgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5221 ggagaggcgg tttgcgtatt gggcgcatgc ataaaaactg ttgtaattca ttaagcattc
     5281 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     5341 ccttgtcgcc ttgcgtataa tatttgccca tggacgcaca ccgtggaaac ggatgaaggc
     5401 acgaacccag ttgacataag cctgttcggt tcgtaaactg taatgcaagt agcgtatgcg
     5461 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     5521 tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat ccaagcagca
     5581 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     5641 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg
     5701 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg
     5761 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg
     5821 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag
     5881 aagcggttgt tggcgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg
     5941 agatctatat ctatgatctc gcagtctccg gagagcaccg gaggcagggc attgccaccg
     6001 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc
     6061 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag
     6121 aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga
     6181 tcggcttccc ggccgcggag ttgttcggta aattgtcaca acgccg
//
