LOCUS       sequence-33275-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL AAGY
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(717..740)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(758..776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(819..838)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gctcgagcgg ccgcttagta ggtaactggc tgcaggtcca agttattatg tgctggggac
       61 atcggattcg gggataggtt tggagaacct gcgtccatgc tgtggttcat ctggtggtca
      121 ctggtttctc catcttcact caggtagcca gcggctgggg tctctggaat attgctctgg
      181 ggctcgatgc ctgcggggaa gttagtgttt tcggggatgg aatggctgta gtcgtccagt
      241 ggggggaact cggccgggat ctctgtgtgg cgtggcacca acacaggagg tagaactggt
      301 gtctctactc tctggtagtg gtagggattc acgcagacct cgtccttctt catattgaag
      361 gcgaactcac acagctccat ggcccgcagc tcgtggtggc tgtgcaggtc tggccatcgc
      421 cacaggcggc agtagatgac atgagggagc cccttccgat gggacacctg caaccggcca
      481 tccagggacc tggggatggt gatgcacttg gtgttgacgt tctgcgtggt gatggccttc
      541 tccagctcgt ccagctgccc cgtcttcttg agtttcttga ccaggctctt gaccgccttc
      601 tcgcaccatt tctcctcctg cccgttctgc tcgcccttct tccagcccag caggcgcttc
      661 acgatcgggg gagtgaaagg caggatggac gatctagagg taccacgcgt gaattctttg
      721 tcatcgtcat ccttatagtc catggtctcg aggctagcct atagtgagtc gtattaagta
      781 ctctagcctt aagagctgta attgaactgg gagtggacac ctgtggagag aaaggcaaag
      841 t
//
