LOCUS       sequence-332752-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, RSF1010 origin, CinRAM 
            activator, PCin promoter, mRFP, Kanamycin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pFCiR2
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      330..724
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1066..1153
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2392..3363
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             3874..4713
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             4700..5551
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     primer_bind     6012..6034
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6026..6043
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6027..6043
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        7192..7241
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             7286..7297
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7304..7981
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(8393..9187)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8504..8523)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9114..9133
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ggtggcactt ttcggggatc ttcaaattcc cgttgcacat agcccggcaa ttcctttccc
       61 tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg atagagaagg
      121 gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct ggccgtcctg
      181 gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac agtctatcgc
      241 ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac attgccagcc
      301 agtgcccgtc actcccgtac taactgtcac gaacccctgc aataactgtc acgcccccct
      361 gcaataactg tcacgaaccc ctgcaataac tgtcacgccc ccaaacctgc aaacccagca
      421 ggggcggggg ctggcggggt gttggaaaaa tccatccatg attatctaag aataatccac
      481 taggcgcggt tatcagcgcc cttgtggggc gctgctgccc ttgcccaata tgcccggcca
      541 gaggccggat agctggtcta ttcgctgcgc taggctacac accgccccac cgctgcgcgg
      601 cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca aaccccgcag aaatacgctg
      661 gagcgctttt agccgcttta gcggcctttc cccctacccg aagggtgggg gcgcgtgtgc
      721 agccccgcag ggcctgtctc ggtcgatcat tcagcccggc tcatccttct ggcgtggcgg
      781 cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac gcatccattg ccgccatgag
      841 ccgatcctcc ggccactcgc tgctgttcac cttggccaaa atcatggccc ccaccagcac
      901 cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc cttgcccgca cccgctgaat
      961 ttcggcattg attcgcgctc gttgttcttc gagcttggcc agccgatccg ccgccttgtt
     1021 gctcccctta accatcttga caccccattg ttaatgtgct gtctcgtagg ctatcatgga
     1081 ggcacagcgg cggcaatccc gaccctactt tgtaggggag ggcgcactta ccggtttctc
     1141 ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg gccattgcat
     1201 ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct tacggcgaaa
     1261 accggcagca ggtcgggcgg ccaatcggcc agggccaagg ccgactacat ccagcgcgaa
     1321 ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg aatccgggca catgccggag
     1381 ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgtatgaacg cgccaatggg
     1441 cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga ccagcagaag
     1501 gcgctggcgt ccgagttcgc ccagcacctg accggtgccg agcgcctgcc gtatacgctg
     1561 gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc cgagcggatc
     1621 aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg caagaccccg
     1681 gagaagggcg gggcacagaa gaccgaagcg ctcaagccca aggcatggct tgagcagacc
     1741 cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca cgacgcccgc
     1801 attgaccaca gaacacttga ggcgcagggc atcgagcgcc tgcccggtgt tcacctgggg
     1861 ccgaacgtgg tggagatgga aggccggggc atccgcaccg accgggcaga cgtggccctg
     1921 aacatcgaca ccgccaacgc ccagatcatc gacttacagg aataccggga ggcaatagac
     1981 catgaacgca atcgacagag tgaagaaatc cagaggcatc aacgagttag cggagcagat
     2041 cgaaccgctg gcccagagca tggcgacact ggccgacgaa gcccggcagg tcatgagcca
     2101 gacccagcag gccagcgagg cgcaggcggc ggagtggctg aaagcccagc gccagacagg
     2161 ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta gccgccgagg tgagcagcgc
     2221 cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg aagctatggc taaccgtgat
     2281 gctggcttcc atgatgccta cggtggtgct gctgatcgca tcgttgctct tgctcgacct
     2341 gacgccactg acaaccgagg acggctcgat ctggctgcgc ttggtggccc gatgaagaac
     2401 gacaggactt tgcaggccat aggccgacag ctcaaggcca tgggctgtga gcgcttcgat
     2461 atcggcgtca gggacgccac caccggccag atgatgaacc gggaatggtc agccgccgaa
     2521 gtgctccaga acacgccatg gctcaagcgg atgaatgccc agggcaatga cgtgtatatc
     2581 aggcccgccg agcaggagcg gcatggtctg gtgctggtgg acgacctcag cgagtttgac
     2641 ctggatgaca tgaaagccga gggccgggag cctgccctgg tagtggaaac cagcccgaag
     2701 aactatcagg catgggtcaa ggtggccgac gccgcaggcg gtgaacttcg ggggcagatt
     2761 gcccggacgc tggccagcga gtacgacgcc gacccggcca gcgccgacag ccgccactat
     2821 ggccgcttgg cgggcttcac caaccgcaag gacaagcaca ccacccgcgc cggttatcag
     2881 ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca ccgctggccc ggcgctggtg
     2941 cagcaggctg gccagcagat cgagcaggcc cagcggcagc aggagaaggc ccgcaggctg
     3001 gccagcctcg aactgcccga gcggcagctt agccgccacc ggcgcacggc gctggacgag
     3061 taccgcagcg agatggccgg gctggtcaag cgcttcggtg atgacctcag caagtgcgac
     3121 tttatcgccg cgcagaagct ggccagccgg ggccgcagtg ccgaggaaat cggcaaggcc
     3181 atggccgagg ccagcccagc gctggcagag cgcaagcccg gccacgaagc ggattacatc
     3241 gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc agcttgcgcg ggccgagctg
     3301 gcacgggcac cggcaccccg ccagcgaggc atggacaggg gcgggccaga tttcagcatg
     3361 tagtgcttgc gttggtactc acgcctgtta tactatgagt actcacgcac agaagggggt
     3421 tttatggaat acgaaaaaag cgcttcaggg tcggtctacc tgatcaaaag tgacaagggc
     3481 tattggttgc ccggtggctt tggttatacg tcaaacaagg ccgaggctgg ccgcttttca
     3541 gtcgctgata tggccagcct taaccttgac ggctgcacct tgtccttgtt ccgcgaagac
     3601 aagcctttcg gccccggcaa gtttctcggt gactgatatg aaagaccaaa aggacaagca
     3661 gaccggcgac ctgctggcca gccctgacgc tgtacgccaa gcgcgatatg ccgagcgcat
     3721 gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc gacgacgaat acgaggcgct
     3781 gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg ggtagtgacc ccgccagcgc
     3841 ctaaccacca actgcctgca aaggaggcaa tcaatggcta cccataagcc tatcaatatt
     3901 ctggaggcgt tcgcagcagc gccgccaccg ctggactacg ttttgcccaa catggtggcc
     3961 ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta aatccatgct ggccctgcaa
     4021 ctggccgcac agattgcagg cgggccggat ctgctggagg tgggcgaact gcccaccggc
     4081 ccggtgatct acctgcccgc cgaagacccg cccaccgcca ttcatcaccg cctgcacgcc
     4141 cttggggcgc acctcagcgc cgaggaacgg caagccgtgg ctgacggcct gctgatccag
     4201 ccgctgatcg gcagcctgcc caacatcatg gccccggagt ggttcgacgg cctcaagcgc
     4261 gccgccgagg gccgccgcct gatggtgctg gacacgctgc gccggttcca catcgaggaa
     4321 gaaaacgcca gcggccccat ggcccaggtc atcggtcgca tggaggccat cgccgccgat
     4381 accgggtgct ctatcgtgtt cctgcaccat gccagcaagg gcgcggccat gatgggcgca
     4441 ggcgaccagc agcaggccag ccggggcagc tcggtactgg tcgataacat ccgctggcag
     4501 tcctacctgt cgagcatgac cagcgccgag gccgaggaat ggggtgtgga cgacgaccag
     4561 cgccggttct tcgtccgctt cggtgtgagc aaggccaact atggcgcacc gttcgctgat
     4621 cggtggttca ggcggcatga cggcggggtg ctcaagcccg ccgtgctgga gaggcagcgc
     4681 aagagcaagg gggtgccccg tggtgaagcc taagaacaag cacagcctca gccacgtccg
     4741 gcacgacccg gcgcactgtc tggcccccgg cctgttccgt gccctcaagc ggggcgagcg
     4801 caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga tcgagttcag
     4861 cggcccggag ccgctgggcg ctgatgatct gcgcatcctg caagggctgg tggccatggc
     4921 tgggcctaat ggcctagtgc ttggcccgga acccaagacc gaaggcggac ggcagctccg
     4981 gctgttcctg gaacccaagt gggaggccgt caccgctgat gccatggtgg tcaaaggtag
     5041 ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat agtggtgggg cgctcaagca
     5101 catacaggac tgcatcgagc gcctttggaa ggtatccatc atcgcccaga atggccgcaa
     5161 gcggcagggg tttcggctgc tgtcggagta cgccagcgac gaggcggacg ggcgcctgta
     5221 cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt ggcggccagc atgtgcgcat
     5281 cagcatggac gaggtgcggg cgctggacag cgaaaccgcc cgcctgctgc accagcggct
     5341 gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc atagatacct tgtgcggcta
     5401 tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc gggtgcgcga
     5461 ggcgttgccg gagctggtcg cgctgggctg gacggtaacc gagttcgcgg cgggcaagta
     5521 cgacatcacc cggcccaagg cggcaggctg acccccccca ctctattgta aacaagacat
     5581 ttttatcttt tatattcaat ggcttatttt cctgctaatt ggtaatacca tgaaaaatac
     5641 catgctcaga aaaggcttaa caatattttg aaaaattgcc tactgagcgc tgccgcacag
     5701 ctccatagcg tttaagggca ccaataacgc cgcacagctc catagcgttt aagggcacca
     5761 ataactgcct taaaaaaatt acgcccagcc ctgccactca tcgcagtacg gcctattggt
     5821 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5881 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5941 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     6001 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgactatt accaattacg
     6061 tcgcgtcatg cggatcctat agggattgat gatgcgcaat tgaacagccc gcgacgcggc
     6121 ggccgagatc gtggtgcagc cgagcctgaa gcgggcggtt ttcaggtaat cgcgtgtggt
     6181 atgctctgat atgcccagga tgaccgaaat atccttgtaa tccttgccga gggcggtcca
     6241 gtgcagacac tcgatctcgc gcggcgacaa tgccggcacc ggatcgtttt cgccatgcag
     6301 ctcatatacg gccttgcggt ggatcagatg ggcgatctcg atccactcat tgcggcagcg
     6361 gcgcacgagc tcggtccatt cgtcggccgg tatatgggca ttcagcgaca gcagggcgcg
     6421 gcgctgcgcc ttgtcggcga cggggatgga gtagccattg tcatcgatgc cgtgtttctg
     6481 ggcgtcgacc agcatggcat aggcctccgg cgtcggttcg acctcgctcc agtcgaaggg
     6541 cagctggcgt tcgaagccct gcttgatgat cggatcgacc ttcacatagc agttgaggag
     6601 gtaacgggaa acccaggcat ccggataggt ggtgcgcacg aagggcgaat cgatcttgct
     6661 cgcgattgtc tgggcgagat ggtaggtgac gaaatcgagg ttatattccg cctggagaat
     6721 acggatggcg gcatccacgt tggccgccgc tttgatctgt tcgaacgcgg actcgaactt
     6781 ttcgctatag gtattctcaa tcattcgccg ttctccgttg acgggcgtct cggacctggg
     6841 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggcgcgccg cccaattatt
     6901 gaaggcctcc ctaacggggg gccttttttt gtttctggtc tcccgcttaa cgatcgttgg
     6961 ctgccctttg tgcgtccaaa cggacgcacg gcgctctaaa gcgggtcgcg atctttcaga
     7021 ttcgctcctc gcgctttcag tctttgtttt ggcgcatgtc gttatcgcaa aaccgctgca
     7081 cacttttgcg cgacatgctc tgatccccct catctggggg ggcctatctg agggaatttc
     7141 cgatccggct cgcctgaacc attctgcttt ccacgaactt gaaaacgcta gctgtcaccg
     7201 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt
     7261 ttaagaactg ggaagagcgc agattaaaga ggagaaatac tagatggctt cctccgaaga
     7321 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     7381 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     7441 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     7501 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     7561 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     7621 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     7681 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     7741 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     7801 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     7861 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     7921 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     7981 ataacgctga tagtgctagt gtagatcgcg ctttccagtc gggaaacctg tcgtgccagc
     8041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
     8101 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     8161 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     8221 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     8281 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     8341 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
     8401 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     8461 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     8521 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     8581 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     8641 cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     8701 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     8761 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     8821 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     8881 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     8941 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     9001 cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     9061 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     9121 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     9181 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     9241 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     9301 agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     9361 atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     9421 tccagatagc ccagtagctg acattcatcc ca
//
