LOCUS       sequence-33276-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL AAGY
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          96..228
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     211..230
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        273..291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             309..332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tagtttatca cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact
       61 taagctgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg
      121 tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat
      181 aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc
      241 agttcaatta cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg
      301 agaccatgga ctataaggat gacgatgaca aagaattcac gcgtggtacc tctagatcgt
      361 ccatcctgcc tttcactccc ccgatcgtga agcgcctgct gggctggaag aagggcgagc
      421 agaacgggca ggaggagaaa tggtgcgaga aggcggtcaa gagcctggtc aagaaactca
      481 agaagacggg gcagctggac gagctggaga aggccatcac cacgcagaac gtcaacacca
      541 agtgcatcac catccccagg tccctggatg gccggttgca ggtgtcccat cggaaggggc
      601 tccctcatgt catctactgc cgcctgtggc gatggccaga cctgcacagc caccacgagc
      661 tgcgggccat ggagctgtgt gagttcgcct tcaatatgaa gaaggacgag gtctgcgtga
      721 atccctacca ctaccagaga gtagagacac cagttctacc tcctgtgttg gtgccacgcc
      781 acacagagat cccggccgag ttccccccac tggacgacta cagccattcc atccccgaaa
      841 acactaactt ccccgcag
//
