LOCUS       sequence-33277-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL* WT
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(638..661)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(679..697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(740..759)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 cggccgcacc tgcgtccatg ctgtggttca tctggtggtc actggtttct ccatcttcac
       61 tcaggtagcc agggggtggg gtctctggaa tattgctctg gggctcgatg cctgcgggga
      121 agttagtgtt ttcggggatg gaatggctgt agtcgtccag tggggggaac tcggccggga
      181 tctctgtgtg gcgtggcacc aacacaggag gtagaactgg tgtctctact ctctggtagt
      241 ggtagggatt cacgcagacc tcgtccttct tcatattgaa ggcgaactca cacagctcca
      301 tggcccgcag ctcgtggtgg ctgtgcaggt ctggccatcg ccacaggcgg cagtagatga
      361 catgagggag ccccttccga tgggacacct gcaaccggcc atccagggac ctggggatgg
      421 tgatgcactt ggtgttgacg ttctgcgtgg tgatggcctt ctccagctcg tccagctgcc
      481 ccgtcttctt gagtttcttg accaggctct tgaccgcctt ctcgcaccat ttctcctcct
      541 gcccgttctg ctcgcccttc ttccagccca gcaggcgctt cacgatcggg ggagtgaaag
      601 gcaggatgga cgatctagag gtaccacgcg tgaattcttt gtcatcgtca tccttatagt
      661 ccatggtctc gaggctagcc tatagtgagt cgtattaagt actctagcct taagagctgt
      721 aattgaactg ggagtggaca cctgtggaga gaaaggcaaa gtggatgtca gtaagaccaa
      781 taggtgccta tcagaaacgc aaaantcttc tctgtctcga caagcccagt ttctattggt
      841 ct
//
