LOCUS       sequence-332773-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for SARS-CoV-2 ORF8 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  TITLE     SARS-CoV-2 protein E. coli expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046735
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..38
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             53..75
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             446..466
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             467..493
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      614..1201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1102..1121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1307..1966
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1373..1392)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2037..2071
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     join(2099..2104,1..14)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(2099..2104,1..13)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
       61 actttaagaa ggagactctt ctatgaaatt tcttgttttc ttaggaatca tcacaactgt
      121 agctgcattt caccaagaat gtagtttaca gtcatgtact caacatcaac catatgtagt
      181 tgatgacccg tgtcctattc acttctattc taaatggtat attagagtag gagctagaaa
      241 atcagcacct ttaattgaat tgtgcgtgga tgaggctggt tctaaatcac ccattcagta
      301 catcgatatc ggtaattata cagtttcctg tttacctttt acaattaatt gccaggaacc
      361 taaattgggt agtcttgtag tgcgttgttc gttctatgaa gactttttag agtatcatga
      421 cgttcgtgtt gttttagatt tcatcgagaa cctgtacttt caaagctacc cgtatgacgt
      481 tcctgattat gcctaaccgg cttatcggtc agtttcacct gatttacgta aaaacccgct
      541 tcggcgggtt tttgcttttg gaggggcaga aagatgaatg actgtccacg acgctatacc
      601 caaaagaaaa agcttgagat ccttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      661 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      721 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      781 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      841 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      961 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1021 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1081 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1141 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1201 attgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
     1261 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     1321 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     1381 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     1441 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     1501 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
     1561 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     1621 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     1681 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     1741 ttcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     1801 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     1861 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     1921 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttg atatcgagct
     1981 cgcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     2041 gaacgctcgg ttgccgccgg gcgtttttta ttggtcatcg atctcgatcc cgcgaaatta
     2101 atac
//
