LOCUS       sequence-332778-       11645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet/Dox-inducible mammalian expression of CARD8 with a site III 
            mutation (R394E) with sgRNA binding site mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11645)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 11645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pInducer20 CARD8-HA PAM R394E
FEATURES             Location/Qualifiers
     source          1..11645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(161..178)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     388..407
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             583..1377
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             583..1377
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(637..656)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1247..1266
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    1554..2142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1607..1627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2025..2036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2217..2450
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2569..2587)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2655..2759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2760..2828,2829..3620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2978..2997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3791..4379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4280..4299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4533..4550
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4790..4811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4826..4831,4832..4849,4850..4856)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4864..4880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4869..4891
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4888..4904
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4888..4904
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4954..4973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5165..5798
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5845..5970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6467..6700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6885..6929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7222..7339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    7394..7412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7436..7454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7478..7496
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7520..7538
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        7571..7609
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7606..7630
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7739..7763
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9381..9405)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9458..9484
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        9570..10781
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     10618..10637
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(10840..11457,11458..11463,11464..11499,11500..11502,
                     11503..11538,11539..11541,11542..11577,11578..11586)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(11639..11645,1..567)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
       61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
      121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
      181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
      241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
      301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
      361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
      421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
      481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
      541 gttttccttt gaaaaacacg atgataagct taccggtcca ccatgattga acaagatgga
      601 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
      661 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
      721 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
      781 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
      841 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
      901 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
      961 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     1021 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     1081 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
     1141 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     1201 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     1261 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     1321 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgatgt
     1381 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     1441 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1501 tgttgttgtt tagtccctcc caattcgata tcaagcttat cgcgatagat cctaatcaac
     1561 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     1621 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     1681 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     1741 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     1801 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     1861 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     1921 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     1981 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2041 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2101 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     2161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     2221 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     2281 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2341 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2401 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     2461 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     2521 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     2581 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3001 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     4621 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     4741 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4801 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4861 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
     4921 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4981 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     5041 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     5101 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     5161 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     5221 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     5281 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     5341 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     5401 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     5461 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     5521 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     5581 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     5641 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5701 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     5761 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     5821 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     5881 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     5941 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     6001 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     6061 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     6121 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     6181 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     6241 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     6301 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     6361 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     6421 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     6481 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     6541 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     6601 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     6661 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6721 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6781 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6841 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6901 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     6961 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     7021 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     7081 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     7141 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     7201 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     7261 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     7321 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     7381 gatcctttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     7441 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     7501 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta
     7561 cccgggtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     7621 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     7681 ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt ggaattctgc agatatcaac
     7741 aagtttgtac aaaaaagcag gcttcgccat ggaaaaaaag gagtgtccag aaaagagtag
     7801 cagcagtgag gaagagctgc cgagacggga cagtggatcc agtaggaaca tagatgcatc
     7861 caaactcatt agactacaag gatcacggaa actgttggtt gacaatagca tacgggaact
     7921 gcaatacaca aaaactggaa ttttttttca ggctgaggcc tgtgtgacaa atgatacggt
     7981 atacagggag ctaccctgtg tttctgagac cctttgtgac atctcacatt ttttccaaga
     8041 agatgatgag acagaggcag agccattatt gttccgtgct gttcctgagt gtcaactatc
     8101 tgggggggac attcccagtg tatcagaaga gcaggaatct tcagagggac aagattcagg
     8161 agacatttgc tcagaagaga atcaaatagt ttcctcttat gcttctaaag tctgttttga
     8221 gatcgaagaa gattataaaa atcgtcagtt tctggggcct gaaggaaatg tggatgttga
     8281 gttgattgat aagagcacaa acagatacag cgtttggttc cccactgctg gctggtatct
     8341 gtggtcagcc acaggcctcg gcttcctggt aagggatgag gtcacagtta ccatcgcctt
     8401 cggcagctgg agtcagcacc tggccctgga cctgcagcac catgaacagt ggctggtggg
     8461 cggccccttg tttgatgtca ctgcagagcc agaggaggct gtcgccgaaa tccacctccc
     8521 ccacttcatc tccctccaag caggtgaggt ggacgtctcc tggtttctcg ttgcccattt
     8581 taagaatgaa gggatggtcc tggagcatcc agcccgggtg gagcctttct atgctgtcct
     8641 ggaaagcccc agcttctctc tgatgggcat cctgctgcgg atcgccagtg ggactcgcct
     8701 ctccatcccc atcacttcca acacattgat ctattatcac ccccaccccg aagatattaa
     8761 gttccacttg taccttgtcc ccagcgacgc cttgctaaca aaggcgatag atgatgagga
     8821 agatcgcttc catggtgtgc gcctgcagac ttcgccccca atggaacccc tgaactttgg
     8881 ttccagttat attgtgtcta attctgctaa cctgaaagta atgcccaagg agttgaaatt
     8941 gtcctacgag agccctggag aaattcagca cttctcaaaa ttctatgctg ggcagatgaa
     9001 ggaacccatt caacttgaga ttactgaaaa aagacatggg actttggtgt gggatactga
     9061 ggtgaagcca gtggatctcc agcttgtagc tgcatcagcc cctcctcctt tctcaggtgc
     9121 agcctttgtg aaggagaacc accggcaact ccaagccagg atgggggacc tgaaaggggt
     9181 gctcgatgat ctccaagaca atgaggttct tactgagaat gagaaggagc tggtggagca
     9241 ggaaaagaca cggcagagca agaatgaggc cttgctgagc atggtggaga agaaagggga
     9301 cctggccctg gacgtgctct tcagaagcat tagtgaaagg gacccttacc tcgtgtccta
     9361 tcttagacag cagaatttgg acccagcttt cttgtacaaa gtggttgata tccagcacag
     9421 tggcggccgc tcgagtctag agggcccgcg gttcgaatac ccatacgacg tcccagacta
     9481 cgcttagtaa tgattaatta aactagaaat tctaccgggt aggggaggcg cttttcccaa
     9541 ggcagtctgg agtaacccac ccaagatctg gcctccgcgc cgggttttgg cgcctcccgc
     9601 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
     9661 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
     9721 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
     9781 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
     9841 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
     9901 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
     9961 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
    10021 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
    10081 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
    10141 atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
    10201 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
    10261 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
    10321 ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta
    10381 cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
    10441 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
    10501 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
    10561 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
    10621 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
    10681 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
    10741 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg taccgagctc
    10801 ggatctccac cccgtaccgg tcctgcagtc gaattcacca tgtctagact ggacaagagc
    10861 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
    10921 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
    10981 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
    11041 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
    11101 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
    11161 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
    11221 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
    11281 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
    11341 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
    11401 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
    11461 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
    11521 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
    11581 gggtaactaa gtaaggatcc gcggccgcac tagaggaatt ccgcccctct cccccccccc
    11641 cctct
//
