LOCUS       sequence-33278-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL* WT
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          99..231
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     214..233
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        276..294
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             312..335
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga
       61 acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
      121 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
      181 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
      241 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
      301 tcgagaccat ggactataag gatgacgatg acaaagaatt cacgcgtggt acctctagat
      361 cgtccatcct gcctttcact cccccgatcg tgaagcgcct gctgggctgg aagaagggcg
      421 agcagaacgg gcaggaggag aaatggtgcg agaaggcggt caagagcctg gtcaagaaac
      481 tcaagaagac ggggcagctg gacgagctgg agaaggccat caccacgcag aacgtcaaca
      541 ccaagtgcat caccatcccc aggtccctgg atggccggtt gcaggtgtcc catcggaagg
      601 ggctccctca tgtcatctac tgccgcctgt ggcgatggcc agacctgcac agccaccacg
      661 agctgcgggc catggagctg tgtgagttcg ccttcaatat gaagaaggac gaggtctgcg
      721 tgaatcccta ccactaccag agagtagaga caccagttct acctcctgtg ttggtgccac
      781 gccacacaga gatcccggcc gagttccccc cactggacga ctacagccat tccatccccg
      841 aaaacactaa cttccccgca ggcatcgagc cccagagcaa
//
