LOCUS       sequence-332780-        1677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of wildtype L. major glcyoprotein-63 from chromosome 28 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Antonia AL, Barnes AB, Martin AT, Wang L, Ko DC
  TITLE     Variation in Leishmania chemokine suppression driven by 
            diversification of the GP63 virulence factor.
  JOURNAL   PLoS Negl Trop Dis. 2021 Oct 28;15(10):e0009224. doi: 
            10.1371/journal.pntd.0009224. eCollection 2021 Oct.
  PUBMED    34710089
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-10-28"; volume: "15(10):e0009224. doi"; 
            pages: " 10.1371/journal.pntd.0009224. eCollection 2021 Oct"
COMMENT     Sequence Label: Lmajor_gp63_28_0570_WT
FEATURES             Location/Qualifiers
     source          1..1677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      982..991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1621..1650
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1651..1668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggtaccgcca ccatgtggtg gaggctgtgg tggttattac tgctgctgct gctgctgtgg
       61 cctatggtgt gggcacaggg acacccagag agggcagact ccgaggagcc aagatgcggc
      121 tttgatgagc tggaggccca caccatcggc acaagggtgt ctggaatcag cagggtggag
      181 ctgccaaccg gagagctggt ggtggcagca gcagcaacag gcgccctgca gccaatcagg
      241 atcgccgtgt tcaccgacga tatctccaac agctcccagc actgcacagc atccggacag
      301 tctaggccaa acttccgggg ctccagggtg acctgttctg ccgcagaggt gctgacacgg
      361 gccaagaaga gagtgctgct ggagctgctg atcccaagcg ccgtgcagct gcaccaggag
      421 aggctgaacg tgcagcgcgt gaacggcaat atcgtggtgg actctagcat ccagaaggat
      481 cgggtgtgcg gccagttctc tatcagagag gagcacatga agaccggcgt gaaggacgcc
      541 gattttgtgc tgtacatgag cgccgcccct acatctggca gcgtgatcgc ctgggccctg
      601 aagtgtcaga acttcgacaa tggccggcca tccgtgggcg tggtgaacat ctctccaaag
      661 tatatcgccg ccgatcccaa gaccgtgaga gtgatcgcac acgaggtgct gcacgcactg
      721 ggcttctccc ggagcgtgtt ccaggagaga aatatgctgg caatggcaag cttccggtcc
      781 aagggaccaa gccccgtgat ctgctccgag aaggtggtgg ccaaggccca gcagcactac
      841 ggctgtaaga cccaggcctt tatggagctg gaggacacag gcgatatcga cgatgcctcc
      901 tctcactgga agcggagaaa tgccaaggac gagctgatgg caggcttctc cggagtgggc
      961 atctattctg ccctgaccat cgccgccatg gaggatacag gctactatca gggcaactac
     1021 gccaaggcag agccaatggc atatggacac gacgcaggat gcaagctgag ctccgatcag
     1081 tgcgtgacca atagcacatc ccagatccct ggcatgtttt gcgacgcccc cgatgcccct
     1141 tggtcttgta ccagcgacag gctgggagtg ggcagatgca tcctgacctc ccacaagtct
     1201 aacctgccta catacttcca gtatttttcc gatccaagac tgggcggccc tgacccactg
     1261 atggattttt gccctgtggt ggaggtggca gagggcacca tgtgcgcagc caccacaaat
     1321 gccctgaagg gcagcgtgta cggcgtgatg tccagatgcg tggacacacc cgtgggcttc
     1381 tctatggacg atagcgccgt gcgccagcac ggaatctgcg tggaggtgca gtgtgacagc
     1441 accaagtact atatcaaggc caacggagca tccgccttct gcgattgtcc acctggctct
     1501 acctacaatc tgagcacact gtctcccagc ttttccaagg gctacctggt gtgccctagc
     1561 tatgagagcg tgtgcgccat caagatcaac gcctctctgt acgaggagta tagcggcttc
     1621 gagcagaagc tgatcagcga ggaggacctg caccaccacc accaccactg aggatcc
//
