LOCUS       sequence-332781-        1857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of wildtype L. major glcyoprotein-63 from chromosome 31 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Antonia AL, Barnes AB, Martin AT, Wang L, Ko DC
  TITLE     Variation in Leishmania chemokine suppression driven by 
            diversification of the GP63 virulence factor.
  JOURNAL   PLoS Negl Trop Dis. 2021 Oct 28;15(10):e0009224. doi: 
            10.1371/journal.pntd.0009224. eCollection 2021 Oct.
  PUBMED    34710089
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-10-28"; volume: "15(10):e0009224. doi"; 
            pages: " 10.1371/journal.pntd.0009224. eCollection 2021 Oct"
COMMENT     Sequence Label: Lmajor_gp63_31_2000_WT
FEATURES             Location/Qualifiers
     source          1..1857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1801..1830
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1831..1848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggtaccgcca ccatgtggtg gaggctgtgg tggttattac tgctgctgct gctgctgtgg
       61 ccaatggtgt gggcagcagc accaagctcc tgtggccact cccagatcgt gcacaagcag
      121 ggccacctgc ctctgacata cgccgccctg aggaacaatg gcaacggcct gcgctatcag
      181 gtggagatgg acctggagag gtggaatggc agcgccaccg atgagcagag ggcagtggag
      241 ccacaggcaa ccgtgacaat ctctagcgac cgggaggatt ctgagtgggc ccctatcaga
      301 atcgccgtgt tcatgccaga cctggaggat gagagcaagt actgtacagc catcggccag
      361 atgagacccg actcctctgg ctccctggtg ccttgcacct ctaacgccga cctgctgaca
      421 gatgccaaga agaccgtgct gatcaacagc atcctgccac aggccgtgaa tatgcacgca
      481 cagcggctga aggtgcggag acctgcagca tccacctcta tcgtggtgaa ttctatgcca
      541 ggcagctact gtggctcctt tacagtgcca accgcacaca gaaccacagg cgtgcccgac
      601 gccgatttcg tggtgtatgt ggcagcagga cccacctcta cacctaagag ctttatcgcc
      661 tgggccgtga catgccagta ctatcctaac accgcaacag tgaccagcag gccagcagtg
      721 ggcgtgatgt acttcaatcc aagatatctg cccacatccg agggcgagac ccaggagcac
      781 gtggacagat atggcggcaa ccctagcggc tccaccaata ggctgaggag ggtggcatcc
      841 cacgagctgc tgcacgccct gggcttcaca agctccgtgt ttaaggcaag gggaatgttt
      901 gcaaccgtgc cctctctgag gggcaagagg aacgtgcctg tgctgaattc tagcgccgtg
      961 agagcagcag caaaggcaaa gtacggcatc agcgacacag aggtgttcta tggagtggag
     1021 ctggaggatc agggagcacc aggcacctct ctgagccact ggaagcggag agccgccaag
     1081 gatgagctga tggcacccgt gttcggactg gcaaggtact cctctctgtc tctggccgcc
     1141 ctggaggaca tgggcttcta taaggtggac ttcagcaagg ccgagccagt ggccctggga
     1201 gcaacagcag gaggcaagct gttcaccgag ccatgcctga cagagggcac ctccaacaca
     1261 cccaccgtgt tttgcgactc cctgtctgcc agcgtgcgca gctgtacagc cgatcggctg
     1321 tccatcggca gatgcgccct gaccacatac agctccgccc tgcctagcta cgcccagtat
     1381 ttccctaacc agccaaccct gggcggctcc ctgtctcaca gcgactattg tcctgtgatc
     1441 cagccactgt ccaatacagg atgctccgga ggctctctgg gagcaatgcc aggctctatc
     1501 accggcgata acgccaggtg ttttgacgcc gataatctgc tggtgaagtt caccttcacc
     1561 cagccaggag caatctgcgc aaaggtgcac tgtaagaacg cctcccgcac ctacgaggtg
     1621 ctggtcatcg gcgccaatag ctggctgtcc tgcggagcag gaggcacagg agtgaccgtg
     1681 cagccagccg tgtctagccc taacgtgttc ctgaagggag gaaggatcgc atgcccacct
     1741 tacgacgacg tgtgctatgc caatccagtg gcctttgccg aggtggaggc accaccaacc
     1801 gagcagaagc tgatcagcga ggaggatctg caccaccacc accaccactg aggatcc
//
