LOCUS       sequence-332782-        9141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion 
            protein for controllable activation with dTAG ligands (dTAG13, 
            dTAGV-1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pLEX305-N-dTAG-CARD8-ZUC
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     238..258
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        286..512
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             513..693
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    740..865
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1358..1591
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1776..1820
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2051..2261
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2073..2269
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2120..2255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2301..2903
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2301..2320)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2797..2817
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2946..3063
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3121..3621
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3540..3558
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3630..3632,3633..3953)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3960..3986
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3960..3986
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3990..4016
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4032..4056
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5203..5227)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5279..5867
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5332..5352)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5750..5761)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5939..6172
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6250..6371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6287..6306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6341..6360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6411..6546
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6566..6585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6567..6585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6595..6612)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6595..6611)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6604..6626)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6753..7208
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6840..6859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7050..7071
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7234..7338
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7339..7407,7408..8199)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7557..7576)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8370..8958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8859..8878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9112..9129
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      241 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      301 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      361 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      421 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      481 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      541 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      601 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      661 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      721 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      781 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      841 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      901 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      961 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1021 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1081 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1141 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1201 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1261 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1321 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1381 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1441 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1501 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1561 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1621 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1681 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1741 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1801 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     1861 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     1921 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     1981 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2041 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt
     2101 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2161 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     2221 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag
     2281 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     2341 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     2401 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     2461 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
     2521 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     2581 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     2641 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     2701 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     2761 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     2821 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     2881 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc
     2941 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     3001 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     3061 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg
     3121 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     3181 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     3241 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     3301 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     3361 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     3421 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     3481 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     3541 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     3601 atcaccgacc tctctcccca ggggctagca tgggagtgca ggtggaaacc atctccccag
     3661 gagacgggcg caccttcccc aagcgcggcc agacctgcgt ggtgcactac accgggatgc
     3721 ttgaagatgg aaagaaagtt gattcctccc gggacagaaa caagcccttt aagtttatgc
     3781 taggcaagca ggaggtgatc cgaggctggg aagaaggggt tgcccagatg agtgtgggtc
     3841 agagagccaa actgactata tctccagatt atgcctatgg tgccactggg cacccaggca
     3901 tcatcccacc acatgccact ctcgtcttcg atgtggagct tctaaaactg gaaggcggct
     3961 acccctacga cgtgcccgac tacgccggct atccgtatga tgtcccggac tatgcaggca
     4021 tcgatagatc aacaagtttg tacaaaaaag caggcttcga aggagataga accatggggc
     4081 ctgaaggaaa tgtggatgtt gagttgattg ataagagcac aaacagatac agcgtttggt
     4141 tccccactgc tggctggtat ctgtggtcag ccacaggcct cggcttcctg gtaagggatg
     4201 aggtcacagt gacgattgcg tttggttcct ggagtcagca cctggccctg gacctgcagc
     4261 accatgaaca gtggctggtg ggcggcccct tgtttgatgt cactgcagag ccagaggagg
     4321 ctgtcgccga aatccacctc ccccacttca tctccctcca agcaggtgag gtggacgtct
     4381 cctggtttct cgttgcccat tttaagaatg aagggatggt cctggagcat ccagcccggg
     4441 tggagccttt ctatgctgtc ctggaaagcc ccagcttctc tctgatgggc atcctgctgc
     4501 ggatcgccag tgggactcgc ctctccatcc ccatcacttc caacacattg atctattatc
     4561 acccccaccc cgaagatatt aagttccact tgtaccttgt ccccagcgac gccttgctaa
     4621 caaaggcgat agatgatgag gaagatcgct tccatggtgt gcgcctgcag acttcgcccc
     4681 caatggaacc cctgaacttt ggttccagtt atattgtgtc taattctgct aacctgaaag
     4741 taatgcccaa ggagttgaaa ttgtcctaca ggagccctgg agaaattcag cacttctcaa
     4801 aattctatgc tgggcagatg aaggaaccca ttcaacttga gattactgaa aaaagacatg
     4861 ggactttggt gtgggatact gaggtgaagc cagtggatct ccagcttgta gctgcatcag
     4921 cccctcctcc tttctcaggt gcagcctttg tgaaggagaa ccaccggcaa ctccaagcca
     4981 ggatggggga cctgaaaggg gtgctcgatg atctccaaga caatgaggtt cttactgaga
     5041 atgagaagga gctggtggag caggaaaaga cacggcagag caagaatgag gccttgctga
     5101 gcatggtgga gaagaaaggg gacctggccc tggacgtgct cttcagaagc attagtgaaa
     5161 gggaccctta cctcgtgtcc tatcttagac agcagaattt ggacccagct ttcttgtaca
     5221 aagtggttac cggttagtaa tgaactagtt aagtcgacaa tcaacgcgtt aagtcgacaa
     5281 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5341 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5401 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5461 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5521 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5581 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5641 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5701 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5761 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5821 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     5881 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     5941 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6001 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6061 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6121 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     6181 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6241 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6301 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6361 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6421 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6481 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6541 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6601 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6661 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6721 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6901 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6961 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7021 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7081 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7141 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7201 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7261 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7321 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7381 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7441 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7501 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7561 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7621 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7681 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7741 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7801 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7861 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7921 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7981 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8041 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8101 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8161 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8221 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9061 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9121 gtgagcgagg aagcggaaga g
//
