LOCUS       sequence-332791-        6098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5058:Dendra
FEATURES             Location/Qualifiers
     source          1..6098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2224..2247
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2504..3304
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3429..4017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3918..3937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggccgaa attgtgttgg atcgggtgac caagagttac
      301 cccgacggcg caggcggtgt cagggcggcc gtcaaagagt tctcgatgac gatcgccgac
      361 ggcgagttca tcatcctggt cggaccgtcc ggatgcggaa agtcgaccac gctcaacatg
      421 attgccggtc tggaggagat cacctccggt gagctgcgca tcggtgggga acgcgtcaac
      481 gagaaggcgc ccaaggaccg tgacatcgcg atggtgttcc agtcctacgc gctctacccg
      541 cacatgacgg tgcggcagaa catcgcattc ccgctcacgc tggccaaggt gcccaaggcc
      601 gagatcgccg ccaaggtcga ggagaccgcg aagatcctcg atctgagcga actgctcgac
      661 cgcaagcccg gtcagctctc gggcggccag cgtcagcgtg tggcgatggg acgcgcgatc
      721 gtgcgcagcc ccaaggcgtt tctcatggac gagccgctga gcaacctgga cgccaagctg
      781 cgcgtgcaga tgcgcgcgga gatctcgcgg ctgcaggatc gcctgggcac caccacggtc
      841 tacgtcacgc acgaccagac cgaggcgatg acgctgggcg accgcgtggt ggtgatgctc
      901 gccggcgagg tgcagcagat cggcacaccc gacgagctct acagcagccc cgccaacctg
      961 ttcgtggccg gtttcatcgg ttcgccggcg atgaacttct tcccggcgac gcgtaccgat
     1021 gtcggtgtgc gcctgccgtt cggcgaggtg acactgaccc cgcacatgct cgacctgctg
     1081 gacaagcagg ccaggcccga gaacatcatc gtcggaatcc ggcccgagca catcgaggac
     1141 tcggcgctgc tcgacggcta cgcgcggatc cgcgcgctga cgttctcggt gcgtgccgac
     1201 atcgtcgaat cgctcggcgc cgacaagtac gtgcacttca ccaccgaggg tgccggcgcc
     1261 gagtcggccc aattggccga actcgccgcg gattcgggtg cgggcaccaa ccagttcatc
     1321 gcccgggtgt ccgcggactc gcgggtgcgc acgggtgagc agatcgaact ggccatcgac
     1381 accaccaagc tgtcgatctt cgacgccgca acgggtctga acctgacccg tgacatcacg
     1441 ccgacggatc ccaccgaagc ggcggggccg gacgccggga agcttggtgc ggccgcgggc
     1501 aacacgcctg gcatcaatct gatcaaggag gacatgcgtg tcaaggtgca tatggagggc
     1561 aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta agggtaagcc ttacgaaggg
     1621 acccagaccg ccaacctcac cgtcaaggag ggcgcccctc tcccattctc gtacgatatc
     1681 ctcactaccg cggtgcatta cgggaaccgc gtgttcacca agtaccccga ggatatccct
     1741 gactacttca agcagagctt cccggaggga tactcctggg agcggacgat gaccttcgaa
     1801 gacaagggta tctgtacgat ccgcagcgac atctcgctgg aaggggactg tttcttccag
     1861 aacgtccgtt tcaaggggac caactttccg ccgaacggcc cggtcatgca gaagaagacc
     1921 ctcaagtggg agccgagcac ggagaagctg cacgttcggg acggcctcct ggtcggcaac
     1981 atcaacatgg cgctgctgct ggaaggcggc ggccactacc tatgcgactt caagaccacg
     2041 tacaaagcga agaaggtcgt ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa
     2101 atcctgggga acgattcgga ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc
     2161 tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg gcggggagaa cctgtatttc
     2221 caggactaca aggacgacga cgacaagtga tttaaataac cccttggggc ctctaaacgg
     2281 gtcttgaggg gttttttgtt aactagcgta cgatcgaatt cctgcagctc acggtaactg
     2341 atgccgtatt tgcagtacca gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg
     2401 cctttgaatg ggttcatgtg cagctccatc agcaaaaggg gatgataagt ttatcaccac
     2461 cgactatttg caacagtgcc gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga
     2521 gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc atcggtcagc ttctcaacct
     2581 tggggttacc cccggcggtg tgctgctggt ccacagctcc ttccgtagcg tccggcccct
     2641 cgaagatggg ccacttggac tgatcgaggc cctgcgtgct acgctgggtc cgggagggac
     2701 gctcgtcatg ccctcgtggt caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc
     2761 cgttacaccg gaccttggag ttgtctctga cacattctgg cgcctgccaa atgtaaagcg
     2821 cagcgcccat ccatttgcct ttgcggcagc ggggccacag gcagagcaga tcatctctga
     2881 tccattgccc ctgccacctt actcgcctgc aagcccggtc gcccgtgtcc atgaactcga
     2941 tgggcaggta cttctcctcg gcgtgggaca cgatgccaac acgacgctgc atcttgccga
     3001 gttgatggca aaggttccct atggggtgcc gagacactgc accattcttc aggatggcaa
     3061 gttggtacgc gtcgattatc tcgagaatga ccactgctgt gagcgctttg ccttggcgga
     3121 caggtggctc aaggagaaga gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg
     3181 gttgatccgc tcccgcgaca ttgtggcgac agccctgggt caactgggcc gagatccgtt
     3241 gatcttcctg catccgccag aggcgggatg cgaagaatgc gatgccgctc gccagtcgat
     3301 tggctgagct catgagcgga gaacgagatg acgttggagg ggcaaggtcg cgctgattgc
     3361 tggggcaaca cgggggatcc actagttcca tgagcgtcag accccgtaga aaagatcaaa
     3421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3541 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3841 cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4141 accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc
     4201 tctagagtcg accaccaagg gcaccatctc tgcttgggcc accccgttgg ccgcagccag
     4261 ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg ccgacggcga gggttccgac
     4321 cgctgcaact cccggtgcaa ccttgtcccg gtctattctc ttcactgcac cagctccaat
     4381 ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat
     4441 cgaaagtagc cagatcaggg atgcgttgca accgcgtatg cccaggtcag aagagtcgca
     4501 caagagttgc agacccctgg aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag
     4561 cggagcgggc gacgggaatc gaacccgcgt agctagtttg gaagaatggg tgtctgccga
     4621 ccacatatgg gccggtcaag ataggttttt accccctctc ggctgcatcc tctaagtgga
     4681 aagaaattgc aggtcgtaga agcgcgttga agcctgagag ttgcacagga gttgcaaccc
     4741 ggtagccttg ttcacgacga gaggagacct agttggcacg tcgcggatgg ggatcgctga
     4801 agactcagcg cagcgggagg atccaagcct catacgtcaa cccgcaggac ggtgtgaggt
     4861 actacgcgct gcagacctac gacaacaaga tggacgccga agcctggctc gcgggcgaga
     4921 agcggctcat cgagatggag acctggaccc ctccacagga ccgggcgaag aaggcagccg
     4981 ccagcgccat cacgctggag gagtacaccc ggaagtggct cgtggagcgc gacctcgcag
     5041 acggcaccag ggatctgtac agcgggcacg cggagcgccg catctacccg gtgctaggtg
     5101 aagtggcggt cacagagatg acgccagctc tggtgcgtgc gtggtgggcc gggatgggta
     5161 ggaagcaccc gactgcccgc cggcatgcct acaacgtcct ccgggcggtg atgaacacag
     5221 cggtcgagga caagctgatc gcagagaacc cgtgccggat cgagcagaag gcagccgatg
     5281 agcgcgacgt agaggcgctg acgcctgagg agctggacat cgtcgccgct gagatcttcg
     5341 agcactaccg gatcgcggca tacatcctgg cgtggacgag cctccggttc ggagagctga
     5401 tcgagcttcg ccgcaaggac atcgtggacg acggcatgac gatgaagctc cgggtgcgcc
     5461 gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa cgccaagacc gtccggtcga
     5521 agcgtcctgt gacggttccg cctcacgtcg cggagatgat ccgagcgcac atgaaggacc
     5581 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
     5641 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
     5701 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
     5761 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
     5821 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
     5881 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
     5941 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
     6001 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
     6061 ggtgaccaca acgcgcccgc tttgatcggg gacgtcga
//
