LOCUS       sequence-332792-        6181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV transfer plasmid for neuronal expression of soma-targeted 
            jGCaMP8m..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: AAV-hSyn-Soma-jGCaMP8m
FEATURES             Location/Qualifiers
     source          1..6181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     336..357
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(463..484)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(724..743)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    1678..2266
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1731..1751)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2149..2160)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2298..2774
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2497..2516)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2814..2954
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2814..2943
                     /label=AAV2 ITR
     rep_origin      3029..3484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3116..3135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3326..3347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3501..3520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3620..3642
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3680..3698)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3766..3870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3871..3939,3940..4731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4089..4108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4902..5490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5391..5410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5552..5681
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        5734..6181
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 tcgagaaacc ggctagagga tccacccgcc accatgcatc atcaccatca tcacacgcgt
       61 cgcaagaaga ccttcaagga ggtggccacc gccgtgaaga tcatcggccg cctgatgggc
      121 ctgaagatca acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
      181 cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
      241 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtggagtcc
      301 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
      361 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
      421 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      481 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
      541 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      601 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      661 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
      721 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      781 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      841 ctggagtaca acctgccgga ccaactgact gaagagcaga tcgcagaata caaagaggct
      901 ttctccctat ttgacaagga cggggatggg acaataacaa ccaaggagct ggggacggtg
      961 atgcggtctc tggggcagaa ccccacagaa gcagagctgc aggacatgat caatgaagta
     1021 gatgccgacg gtgacggcac aatcgacttc cctgagttcc tgacaatgat ggcaagaaaa
     1081 atgaaataca gggacacgga agaagaaatt agagaagcgt tcggtgtgtt tgataaggat
     1141 ggcaatggct acatcagtgc agcagagctt cgccacgtga tgacaaacct tggagagaag
     1201 ttaacagatg aagaggttga tgaaatgatc agggaagcag acatcgatgg ggatggtcag
     1261 gtaaactacg aagagtttgt acaaatgatg acagcgaagg agctcggggg cagcggtggg
     1321 tctggaggga ctgggggtag cggtggtagc ggtgggacag ggggaagcgg cggtagcggt
     1381 gggacactgg aaatcgaggc cgctttcctg gagcaggaga acaccgccct ggagacagag
     1441 gtggctgagc tggagcagga ggtgcagcgg ctggagaaca tcgtgtctca gtacgagacc
     1501 agatacggac cactgggatc cctggagatc cgggccgctt ttctgaggag acggaacacc
     1561 gccctgagga caagggtggc tgagctgagg cagagagtgc agcggctgcg caacatcgtg
     1621 agccagtacg agacacgcta cggacctctg taagaattcg atatcaagct tatcgataat
     1681 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     1741 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     1801 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     1861 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     1921 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     1981 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2041 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2101 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     2161 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     2221 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     2281 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     2341 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     2401 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     2461 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     2521 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     2581 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     2641 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     2701 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     2761 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     2821 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2881 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2941 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     3001 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     3121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3241 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     3301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     3361 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     3421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     3481 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     3541 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3601 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3661 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3721 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3781 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3841 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3901 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3961 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4021 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4081 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4141 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4201 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4261 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4321 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4381 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4441 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4501 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4561 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4621 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4681 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4741 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4801 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     4861 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     4921 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     4981 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5041 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5101 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5161 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5221 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5281 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5341 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5401 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5461 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5521 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     5581 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     5641 gcgcagagag ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtttaat
     5701 taagtgtcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat
     5761 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     5821 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     5881 aggctcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     5941 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     6001 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     6061 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     6121 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     6181 g
//
