LOCUS       sequence-332793-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5261:Dendra
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1870..1893
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2150..2950
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3075..3663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3564..3583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgagtgaa ctgcggttga tggcggtgca tgcccacccg
      301 gacgacgagt ccagcaaggg tgcggcaacc accgcgcgct acgcggccga gggtgcccgc
      361 gtcatggtgg tgaccctgac cggcggcgag cgcggagaca ttctcaatcc ggcgatggac
      421 ctgcccgagg tccacggccg gatcgccgag gtgcgccgtg acgagatggc caaagcggcc
      481 gagatcctgg gtgtcgagca ccactggctg ggtttcgtcg actccggatt gccggagggt
      541 gacccgctgc cgccgctgcc cgacgggtgt ttcgccctgg tcccgctcga ggagcccgtc
      601 aagcgtctgg tgcgggtgat ccgcgagttc cgcccgcacg tgatgaccac gtacgacgag
      661 aacggtggtt acccgcatcc cgaccacatc cgctgccacc aggtgtcggt ggccgcgtac
      721 gaggccgcgg ccgaccacct gctgtatccc gacgccggtg agccgtgggc ggtgcagaag
      781 ctgtactaca accatggctt cctccgtcag cgcatgcagc tcctgcagga ggagttcgcc
      841 aagaacgggc aggagggccc gttcgccaaa tggctcgagc actgggatcc cgacaacgac
      901 gtgttcgcca accgggtgac cacccgcgtg cactgcgcgg agtacttcca ccagcgtgac
      961 gacgcgttgc gtgcgcacgc gacacagatc gatcccaagg gcgacttctt ccacgccccg
     1021 atcgagtggc agcagcggtt gtggccgacc gaggagttcg agctcgcgcg ggcccgcgtg
     1081 ccggtcacgc tgcccgagga cgacctgttc aagggggtcg agccaaagct tggtgcggcc
     1141 gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa ggtgcatatg
     1201 gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg taagccttac
     1261 gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc attctcgtac
     1321 gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta ccccgaggat
     1381 atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg gacgatgacc
     1441 ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg ggactgtttc
     1501 ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt catgcagaag
     1561 aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg cctcctggtc
     1621 ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg cgacttcaag
     1681 accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt ggaccaccgc
     1741 atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga gcacgctgtg
     1801 gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg ggagaacctg
     1861 tatttccagg actacaagga cgacgacgac aagtgattta aataacccct tggggcctct
     1921 aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg cagctcacgg
     1981 taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc acgctgaaaa
     2041 tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg ataagtttat
     2101 caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc
     2161 ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg gtcagcttct
     2221 caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc gtagcgtccg
     2281 gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc tgggtccggg
     2341 agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg atcctgccac
     2401 gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc tgccaaatgt
     2461 aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag agcagatcat
     2521 ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc gtgtccatga
     2581 actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga cgctgcatct
     2641 tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca ttcttcagga
     2701 tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc gctttgcctt
     2761 ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg gtcatgcctt
     2821 tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac tgggccgaga
     2881 tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg ccgctcgcca
     2941 gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca aggtcgcgct
     3001 gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc cgtagaaaag
     3061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     3241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3481 acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3721 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3781 gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg cggtacccgg
     3841 ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc cgttggccgc
     3901 agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt
     3961 tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc
     4021 tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt
     4081 cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga
     4141 gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct
     4201 gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc
     4261 tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct gcatcctcta
     4321 agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc acaggagttg
     4381 caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc ggatggggat
     4441 cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg caggacggtg
     4501 tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc tggctcgcgg
     4561 gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg gcgaagaagg
     4621 cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg gagcgcgacc
     4681 tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc tacccggtgc
     4741 taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg tgggccggga
     4801 tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg gcggtgatga
     4861 acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag cagaaggcag
     4921 ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc gccgctgaga
     4981 tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc cggttcggag
     5041 agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg aagctccggg
     5101 tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc aagaccgtcc
     5161 ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga gcgcacatga
     5221 aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg acgcagggca
     5281 accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc aagatcggtc
     5341 ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag
     5401 gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg atggcgatga
     5461 agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg
     5521 ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc taaagagggg
     5581 ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac cgccgctctg
     5641 tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc cgccagaaat
     5701 ctagacggtg accacaacgc gcccgctttg atcggggacg tcga
//
