LOCUS       sequence-33280-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV expression of humanized ChR2(H134R) fused to EYFP driven by 
            CaMKIIa promoter for optogenetic activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Lee JH, Durand R, Gradinaru V, Zhang F, Goshen I, Kim DS, Fenno LE, 
            Ramakrishnan C, Deisseroth K
  TITLE     Global and local fMRI signals driven by neurons defined 
            optogenetically by type and wiring.
  JOURNAL   Nature. 2010 Jun 10. 465(7299):788-92.
  PUBMED    20473285
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Jun 10. 465(7299):788-92."
COMMENT     Sequence Label: pAAV-CaMKIIa-hChR2(H134R)-EYFP
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccttgctcac catggtggcg gccgctggca cggctccggc ctcggcttcg tcttcgacga
       61 gagtctcgac ctcgatctcc gttccgccga tgttcagttt ggtggttttg cggatatctc
      121 cgtgaatcaa tatgtgctcg tggatcagga cgcgcaggta gtgtcccaac aacccccaac
      181 aatttttact catcagatca ataatcgtgt gacctacggt ggagccatag acgctcagga
      241 cgccaaaacc ttcgggcccc aaaatgaaga gaattgggaa cataccccag ctcacgaaaa
      301 acagccatgc catgccggtc acgacctggc ggcaccgacc ctttggcaca gtatgataac
      361 cctcgatata tgctttggcg gcgtgaaaaa atgtgttcgc gccatagcac aatccaagac
      421 aaaagaagat gactttaaca tagccggttg ccatggcgct ggtagccccc cacacgatag
      481 tcccgatgtc tgagacaagg agtcccatgg ttctcctgct gtagtcgttg ctcaggccgg
      541 tgaggttgct caggcggata aggatgacag gacaagtgag cagccactct gcatagcgca
      601 gccactgcac ccggtgtcct gtggcaaggt agagcataga gggattctta aactcaaaaa
      661 agaactcgag aatcacctta accatttcaa tggcgcacac atagatctcc tcccagccgc
      721 atgtagattt ccaggtttgg taggcataga acatcagcag caaaatgctg aatcctgctg
      781 caagccactg caggacattt gacgcggtct gagcgccgtt cgtgccgcga gattcaatcc
      841 atccggcaca gtaacattga tcctcaggga ccaggacgga cccgttcacc accacaggat
      901 tagtaacgaa caaaagttcg cgtccgacgg cagacaaagc gccgcca
//
