LOCUS       sequence-332822-        5510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5073:Dendra
FEATURES             Location/Qualifiers
     source          1..5510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1636..1659
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1916..2716
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2841..3429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3330..3349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatggccagc tcagccgagg cgatcgtcac gcacgcagaa
      301 cggtcgatct ccgaggacgc gatcgtcgcg gcggcccgcg agcgcgccga cgacatcggc
      361 gccggagccg tcacccccgc ggtcggtgcg ctgctgagcg tgctggcgcg cctcaccggc
      421 ggtcgcgccg tggtcgaggt cggcaccggc gcgggcgtga gcggcctgtg gctgctgtcg
      481 ggtatgcgcg acgacggcgt gctgaccacg atcgacgtcg aacccgagca ccagcgcatc
      541 gccaagcagg gcttctccga ggccggcgtc gggccggggc gcacccggct gatcagcggg
      601 cgcgcgcagg aagtgctgac gcgcctggcc gacgagtcct acgacctcgt gttcatcgac
      661 gccgacccgg tcgaccagcc gcagttcgtg gtcgagggtg tgcgtctgtt gcgttcgggc
      721 ggcgcgatcg tggtgcaccg cgccgcgctg ggcggacgcg cgggcgacgc cgacgcgcgc
      781 gacgccgagg tcaccgcggt gcgcgaggcc gcgcgcctga tcgccgagga cgagcggctc
      841 accccggtgc tcatccccct cggcgacggc cttctcgccg ccgtccgcga caagcttggt
      901 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
      961 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1021 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1081 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1141 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     1201 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     1261 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     1321 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     1381 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     1441 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     1501 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     1561 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     1621 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     1681 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     1741 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     1801 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     1861 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     1921 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     1981 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2041 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2101 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2161 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2221 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     2281 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     2341 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     2401 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     2461 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     2521 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     2581 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     2641 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     2701 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     2761 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     2821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3241 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3541 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     3601 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     3661 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     3721 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     3781 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     3841 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     3901 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     3961 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4021 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4081 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4141 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     4201 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     4261 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     4321 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     4381 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     4441 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     4501 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     4561 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     4621 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     4681 aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg
     4741 ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg agcctccggt
     4801 tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg acgatgaagc
     4861 tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga
     4921 ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg atccgagcgc
     4981 acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc
     5041 agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga
     5101 tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc
     5161 aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg
     5221 cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca
     5281 agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa
     5341 gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc
     5401 gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc cgttcccgcc
     5461 agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga
//
