LOCUS       sequence-332825-        6428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5447:Dendra
FEATURES             Location/Qualifiers
     source          1..6428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2554..2577
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2834..3634
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3759..4347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4248..4267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgaccgcc ctcgacaccg atacgccgac ggctggtcgc
      301 tcggctccac tgatcagtcc cgggcccgtg atcccgcccc ccgatttcgg tccgctggac
      361 cgcgcgcagg gctgggcgat gacggccatc atcactgctc tggccgcgat cacccggttc
      421 ttgaacctgg ggtcgcccac cgatgccggc acgccgatct tcgacgagaa gcactacgcg
      481 ccgcaggcct ggcaggtgct gcacaacgac ggtgtcgagg acaatcccgg ctacgggctg
      541 gtggtgcacc cgcccgtcgg caagcagttg atcgccatcg gcgagtggct gttcggttac
      601 aacggcctgg gctggcggtt ctcgggcgcg gtgtgcggcg tcatcatcgt gatgctggtg
      661 acccgcatcg cgcggcgcat cagccgctcg acgctggtcg gcgcgatcgc cgggttgttg
      721 atcatcgccg acggggtcag cttcgtgtca tcgcgcacag cgctgctcga cgtgttcctg
      781 gtgatgttcg cggtcgcggc gttcgcgtgc ctcatggtcg accgcgacca ggtgcgcgaa
      841 cggatgtacc acgcgtttct cgacggccgt atcgccgaga cccgctgggg tacccggctc
      901 ggtgtgcgct ggtggcgttt cggtgcgggc gtgctgctgg gcctggcgtg cgcgacgaag
      961 tggtccgggc tgtacttcgt gttgttcttc ggcgtcatga cgctcgtgtt cgacgccatc
     1021 gcgcgcaagc agtaccacgt gccgcatccg tggcggggga tgctgagacg cgatctggga
     1081 cctgccgcat acgtcttcgg gctgatcccg ttcgcggtgt acctggcgtc gtacgcgccg
     1141 tggttcgcct ccgagaccgc cgtcaaccgc tacgaggtcg ggcgctccat cgggccggac
     1201 agcatcctgc ccatccccga cgcactgcgc tcgctgtggc actacaccca tgccgcctac
     1261 cgcttccact cgaacctgac caacgccgac ggcaatcacc acccgtggga gtcgaagccg
     1321 tggacgtggc cgatgtcgct gcgccccgtg ctgtacgcga tcgacaacca ggacgtgccc
     1381 ggctgcggcg cgcagtcctg cgtcaaggcc gtgatgctcg tcggcacgcc cgccatgtgg
     1441 ttcatcgcgg tgccggtgct cggctgggcg ctgtggcgga ccgtggtgcg acgtgactgg
     1501 cgctacggcg cggtgctcgt cgggtacatg gccgggttcc tgccgtggtt cgccgacatc
     1561 gaccgccaga tgtacttctt ctacgccacg gtgatggcgc cgttcctggt gctcgcgatc
     1621 gcgctgatcc tcggcgacat cctctacaaa cccaaccaga accccgaacg ccgcacactc
     1681 ggcctgctga cggtgtgttt ctatgtcgcg ctggtgatca cgaacttcgc ctggatgtac
     1741 ccgatcctga cgggcctgcc gatctcacag accacctgga acctgcagat ctggttgccg
     1801 agctggcgca agcttggtgc ggccgcgggc aacacgcctg gcatcaatct gatcaaggag
     1861 gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag
     1921 ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac cgtcaaggag
     1981 ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta cgggaaccgc
     2041 gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt cccggaggga
     2101 tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat ccgcagcgac
     2161 atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac caactttccg
     2221 ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac ggagaagctg
     2281 cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc
     2341 ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt ccagctgccc
     2401 gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga ctacaacaag
     2461 gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc
     2521 gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga cgacaagtga
     2581 tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt aactagcgta
     2641 cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
     2701 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
     2761 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg
     2821 ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa
     2881 agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt
     2941 ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc
     3001 cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga
     3061 cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga
     3121 cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc
     3181 ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt actcgcctgc
     3241 aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca
     3301 cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc
     3361 gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga
     3421 ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa
     3481 ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac
     3541 agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg
     3601 cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg
     3661 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc actagttcca
     3721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3901 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4141 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     4201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4381 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4441 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4501 aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg gcaccatctc
     4561 tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa
     4621 cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa ccttgtcccg
     4681 gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc
     4741 aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca
     4801 accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg aaagaaaaat
     4861 ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt
     4921 agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt
     4981 accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga
     5041 agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga gaggagacct
     5101 agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg atccaagcct
     5161 catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac gacaacaaga
     5221 tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag acctggaccc
     5281 ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag gagtacaccc
     5341 ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac agcgggcacg
     5401 cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg acgccagctc
     5461 tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc cggcatgcct
     5521 acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc gcagagaacc
     5581 cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg acgcctgagg
     5641 agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca tacatcctgg
     5701 cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac atcgtggacg
     5761 acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg
     5821 tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg cctcacgtcg
     5881 cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc cccgaggcat
     5941 tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc aagtcgctga
     6001 agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg
     6061 gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc
     6121 acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta
     6181 tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca
     6241 aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc
     6301 gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg
     6361 agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg
     6421 gacgtcga
//
