LOCUS       sequence-332826-        8327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5044:Dendra
FEATURES             Location/Qualifiers
     source          1..8327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4453..4476
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4733..5533
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5658..6246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6147..6166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgttcgtc accgaggacg gcggtagcgg accgcgggtg
      301 atctacagcg cgtcggacct cgcggccgcc gcgcgctgtg agtacgcgct gctgcgggcg
      361 ttcgacgccc agctgggacg cggaccggcg gtgtcttcgg aggacgaact tctcaagcgc
      421 accgcgcaac tcggcgggga gcacgaacag cggcatctcg acgagttgcg cgagggctcg
      481 gagatcacgg tcatcgggcg cccgcgctac accgtgccgg gcctcacggc cgcggccaac
      541 gcgacgctcg ccgcggtcac ccagcgtgca ccggtgatct atcaggccgc gatgttcgac
      601 gggcggttcg ccgggttcgc ggatttcctg atcctctccg acgggcagta ccgcctgcgc
      661 gacaccaagc tggcccgctc ggtcaaggtc gaggcgctgc tgcagctcgc ggcgtacagc
      721 gagacgctga gcgccgcggg cgtacccgtc gcgccggagg tcgaactggt gctcggcgac
      781 ggcgccgtcg ccacctaccc cgtcgacgaa ctgctgcccg tgtaccggcc gcgccgcgcg
      841 gcgcttgagc acctgctcga cgcgcacctg gcctccgggg aaccggtcag ctgggaggac
      901 accgaaatcc gcgcgtgctt ccgctgcccc gagtgcgagg tccaggtgcg tgcgcacgac
      961 gacctgctgc tcgtcgcggg tatgcgcacc agccagcgcg cccggttgat cgacgcgggc
     1021 atcaccacgg tcgccgatct cgccgcgcgt cgcgacgacg agccggtcga gggcctgcac
     1081 ccacgcatcg cgcaggggct gaccgcgcag gcgcggttgc agcgcgcacc gcggaccggc
     1141 aagaaaccgc cgtacgaggt ggtggacccg cagccgctca tgtcgctgcc caaccccgac
     1201 cgcggtgatc tgttcttcga cttcgagggt gacccgctgt ggaccgacga cggcgtgcag
     1261 tggggcctgg agtacatgtt cggcgtgctg ggcaccgcgc cgcgggccga cgaggacttc
     1321 cacccgctgt gggcccacaa ccgccgcgag gagcggcagg cgctgcgcga cttcctggcg
     1381 ctggtgcgca aacgccgcaa gcgtcatccg cacatgcacg tctaccacta cgcggcgtac
     1441 gagaagaccg cgctgctgcg cctggccggg cgttacggcg aaggcgagga cgaggtcgac
     1501 gaactgctgc gcgcaggtgt gctggtggac ctgtatccgc tggtgcgcaa gagcattcgc
     1561 gtgggaaccg agaactacag cctgaaatcc cttgagccgc tgtacatggg tgcgcagttg
     1621 cgtgacggcg acgtcaccac cgcgaccgat tccatcacgc agtacgggct ctactgcacg
     1681 ctgcgcgccg agggccgcga cgacgaggcc gcgctggtgc tcaaggagat cgaggattac
     1741 aacctctacg actgcgtgtc gacgcgcaag ctgcgggact ggctgatcaa ccgggcgatc
     1801 gagtgcagcg tgccgcccct tggcgcgcaa ccggtcggcc gcgacaccgc acccgaaccc
     1861 gaggaccaga tcgcgcgcac gctgcgccgc ttcgtcggcg acgacgagcg caccccgcag
     1921 cagcaggctg tcgccatgat cgcggccgcg cggggctacc accgtcgcga ggacaagccg
     1981 ttctggtgga gccacttcga ccggctcaac cacccggtcg aggaatggtc tgacggcgca
     2041 ggcgttttcg tcgccgacac cgcgcacgtc gaggtcgact ggcacacccc gccgcgggcg
     2101 cgcaagcccc agcggtgggt gcgcctcacc ggcgccatgg ccgccgggga actcagccgc
     2161 gagatgtacg cgctctacga gccgccggcc ccctccggct tgaccgacga tcccgagcgc
     2221 cgcgcgttcg ggtcggtcca ggtgctcgag gccgacgatg cgtccgcacc caccgaggtg
     2281 ctgatctgcg agcgggaacc caaggagggc ggcacctttc accagttgcc gttcgcgctg
     2341 acgccgggcc cgcccatccg caccaccgcg ctgcagaatt ccatcgaggc cacggccgcg
     2401 cagatcgccg aaggacttcc ccggctaccc ggcaacgccg tcgtcgacat cctgctgcgc
     2461 aggccgccgc gcacccgcag cggcggcccg ctgccacacg gccaggcagc caagcacgag
     2521 ctgatcaccg cggcactgct cgacctcgac tcgtcctacc tcgcggtgca cggtccaccc
     2581 ggcacgggca agacattcac gtctgcggcc gtgatcgcac ggctggtcaa tcgacaccat
     2641 tggcgcatcg gcgtggtcgc acaggcacat gccgtggtgg agaacctttt ccgcgacgtc
     2701 atcgccgcgg acgtgccggc cgacgccgtg gccaagaagc cccatggtga caacgcgtgc
     2761 tggaccgaac tgagcgagaa ggaatatccc gacttcctcg atcagcacac cggcaccggc
     2821 tgcgtgatcg gcggcaccgc atgggatttc gcgaacccgg tacgcatccc gcccggcagt
     2881 ctggatctgc tggtggtcga ggaggccggc cagtacagcc tggccaacac catcgcggtc
     2941 gcccccgcgg cgcacaacct gctgctgctg ggcgacccgc agcagctccc ccaggtcagc
     3001 cagggcacac atcccgaacc cgtcgacacc tcggcgctgg gctggctcgt ggacggccac
     3061 cacacgctgc ccgccgaact cggctacttc ctggactgct cgtaccgcat gcaccccgcg
     3121 gtgtgcgcgg cggtgtcgcg gctgtcctac gacaaccgcc tgcgctcggt cgacgacgtc
     3181 accgcggcgc gccacctcga cggccaggaa cccggcgtgc acgtactgct ggtcgaccac
     3241 gacggcaacg ccaccgacag cccggaggag gcccaggtga tcgtcgccga gatccggcga
     3301 ctgctcggcg cggcctggac cgacgagcgc ggcaccgtgc cgctgggcca ggaccacgtg
     3361 ctggtggtga cgccgtacaa cgcacaggtg gtgacgatgc gccggtatct cgacgcggcc
     3421 ggtctcaccg acgtgcgtgt gggcacggtg gacaagttcc agggccagca ggcgcccgtg
     3481 gtgttcatct cgatggtggc gtcctcggtc gacgatgtgc cgcgcggaat ctcattcctg
     3541 ctcaaccgca accgcctcaa cgtcgcggtg agccgcgcca agtacgccgc ggtgatcgtg
     3601 cggtcggccg cgctcaccga atacctgccc tcgaccccgg ccggtgtcgt ggaactcggc
     3661 gcgttcttgg ccctacccga cgccctaccc gacgccctga acaccgtgaa gcttggtgcg
     3721 gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt caaggtgcat
     3781 atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa gggtaagcct
     3841 tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct cccattctcg
     3901 tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag
     3961 gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga gcggacgatg
     4021 accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga aggggactgt
     4081 ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc ggtcatgcag
     4141 aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga cggcctcctg
     4201 gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct atgcgacttc
     4261 aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac
     4321 cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta cgagcacgct
     4381 gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg cggggagaac
     4441 ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc ccttggggcc
     4501 tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc ctgcagctca
     4561 cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga
     4621 aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt
     4681 tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc
     4741 agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct
     4801 tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt
     4861 ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc
     4921 gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc
     4981 cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc gcctgccaaa
     5041 tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat
     5101 catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg cccgtgtcca
     5161 tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca
     5221 tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca ccattcttca
     5281 ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc
     5341 cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc
     5401 ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg
     5461 agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg
     5521 ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg gcaaggtcgc
     5581 gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga ccccgtagaa
     5641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6061 gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6361 tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc
     6421 cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca ccccgttggc
     6481 cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc cgacggcgag
     6541 ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct tcactgcacc
     6601 agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt
     6661 tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga
     6721 agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc gaaaacaccc
     6781 tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg aagaatgggt
     6841 gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg gctgcatcct
     6901 ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag
     6961 ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt cgcggatggg
     7021 gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac ccgcaggacg
     7081 gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa gcctggctcg
     7141 cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac cgggcgaaga
     7201 aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc gtggagcgcg
     7261 acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc atctacccgg
     7321 tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg tggtgggccg
     7381 ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc cgggcggtga
     7441 tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc gagcagaagg
     7501 cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc gtcgccgctg
     7561 agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc ctccggttcg
     7621 gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg atgaagctcc
     7681 gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac gccaagaccg
     7741 tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca
     7801 tgaaggaccg tacgaagatg aacaagggcc ccgaggcatt cctggtgacc acgacgcagg
     7861 gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg
     7921 gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg
     7981 caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga
     8041 tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc
     8101 tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag
     8161 gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct
     8221 ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccaga
     8281 aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcga
//
