LOCUS       sequence-332827-        6011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_5149:Dendra
FEATURES             Location/Qualifiers
     source          1..6011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        228..246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2137..2160
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2417..3217
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3342..3930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3831..3850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatattggat cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga
       61 tcggccgcat ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg
      121 cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc
      181 agtgatagag tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac
      241 tatagggaga aggaggccat tatgttgctg gtcgtcagca tcctggcgcg gctggcatgg
      301 acctacctcg tgcccaacgg cgccaacttc gtcgacctgc acgtctacgt gggcggcgcg
      361 gacgccctcg atggccccgg cgcgctctac gactacgtct atgccgacca gacaccggac
      421 ttcccgctgc cgttcacgta tccgccgttc gccgcgatcg tgttctaccc gctgcacctg
      481 ctgccgttcg gggtggtggc gttcatctgg cagatcggga tcatcgccgc gctctacggc
      541 gtcgtgcgtg tcagccagcg gctcatgggg ttgcagtccc aacggcgcgt cgcgatgctg
      601 tggaccgcgc tgggcatctg gaccgaaccg ctgcgcagca cattcgacta cggccaggtc
      661 aatgtcgtgc tggtgctcgc ggtgctctgc gcggtgtcga cgacgcggtg gtggctttcg
      721 ggtctgctcg tcgggttggc ggccggcatc aaactcacgc ccgcggtcgc gggcctgtac
      781 ttcctcgggg cgcgccgctg ggccgcggtc gcgtgctcgg cggcggtgtt cttcgcgacg
      841 gtcggcgtgt cgtggctcgt ggtgggtgcg caggcccgcc ggtacttcac cgaactgctg
      901 ggcgacgcgg accggatcgg gcccatcggc acgtcgttca accagtcgtg gcgcggcggg
      961 atctcacgga tcctgggtca cgacgcggga ttcgggccac tggtgctgat cggtatcggc
     1021 atcaccgcgg tcctcgcgct gctggcgtgg cgagcgatcg gcggcgcgca ggatcgactc
     1081 ggcggcatcc tcgtggtgag tctgttcggg ctcgtgctct cgccgatctc gtggacgcac
     1141 cactgggtgt ggctgatccc gctgatgatg tggctgctgc acgggccgtt gtcggccctg
     1201 cgcggcgcgc ggatcctggg ctggggctgg ctcgcgctga ccctgctggg tgtcccgtgg
     1261 ctgctcagtt tcgcgcagcc caccatctgg gagatcggca ggccgtggta cctggcctgg
     1321 gccgggctgg tctacatcgt cgcgacattg gcgacgctcg ggtggatcgc cttcagtcgg
     1381 aaaggatccg gtaagcttgg tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag
     1441 gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga acggtcacgc cttcgtgatc
     1501 gagggggagg gtaagggtaa gccttacgaa gggacccaga ccgccaacct caccgtcaag
     1561 gagggcgccc ctctcccatt ctcgtacgat atcctcacta ccgcggtgca ttacgggaac
     1621 cgcgtgttca ccaagtaccc cgaggatatc cctgactact tcaagcagag cttcccggag
     1681 ggatactcct gggagcggac gatgaccttc gaagacaagg gtatctgtac gatccgcagc
     1741 gacatctcgc tggaagggga ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt
     1801 ccgccgaacg gcccggtcat gcagaagaag accctcaagt gggagccgag cacggagaag
     1861 ctgcacgttc gggacggcct cctggtcggc aacatcaaca tggcgctgct gctggaaggc
     1921 ggcggccact acctatgcga cttcaagacc acgtacaaag cgaagaaggt cgtccagctg
     1981 cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg ggaacgattc ggactacaac
     2041 aaggtcaagt tgtacgagca cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg
     2101 ttcgaaggcg gcggcgggga gaacctgtat ttccaggact acaaggacga cgacgacaag
     2161 tgatttaaat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gttaactagc
     2221 gtacgatcga attcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg
     2281 gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc
     2341 atcagcaaaa ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc
     2401 gtgctatgat cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg
     2461 aaaagccgag ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct
     2521 ggtccacagc tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga
     2581 ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct
     2641 ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc
     2701 tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc
     2761 agcggggcca caggcagagc agatcatctc tgatccattg cccctgccac cttactcgcc
     2821 tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg
     2881 acacgatgcc aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt
     2941 gccgagacac tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa
     3001 tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca
     3061 gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc
     3121 gacagccctg ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg
     3181 atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgagc ggagaacgag
     3241 atgacgttgg aggggcaagg tcgcgctgat tgctggggca acacggggga tccactagtt
     3301 ccatgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3481 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3721 ctacagcgtg agctatgaga aagcgccacg cttcccgagg ggagaaaggc ggacaggtat
     3781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4081 cgcaacgcgt gcggccgcgg tacccgggga tcctctagag tcgaccacca agggcaccat
     4141 ctctgcttgg gccaccccgt tggccgcagc cagctcgctg agagccgtga acgacagggc
     4201 gaacgccagc ccgccgacgg cgagggttcc gaccgctgca actcccggtg caaccttgtc
     4261 ccggtctatt ctcttcactg caccagctcc aatctggtgt gaatgcccct cgtctgttcg
     4321 cgcaggcggg gggctctatt cgtttgtcag catcgaaagt agccagatca gggatgcgtt
     4381 gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt tgcagacccc tggaaagaaa
     4441 aatggccaga gggcgaaaac accctctgac cagcggagcg ggcgacggga atcgaacccg
     4501 cgtagctagt ttggaagaat gggtgtctgc cgaccacata tgggccggtc aagataggtt
     4561 tttaccccct ctcggctgca tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt
     4621 tgaagcctga gagttgcaca ggagttgcaa cccggtagcc ttgttcacga cgagaggaga
     4681 cctagttggc acgtcgcgga tggggatcgc tgaagactca gcgcagcggg aggatccaag
     4741 cctcatacgt caacccgcag gacggtgtga ggtactacgc gctgcagacc tacgacaaca
     4801 agatggacgc cgaagcctgg ctcgcgggcg agaagcggct catcgagatg gagacctgga
     4861 cccctccaca ggaccgggcg aagaaggcag ccgccagcgc catcacgctg gaggagtaca
     4921 cccggaagtg gctcgtggag cgcgacctcg cagacggcac cagggatctg tacagcgggc
     4981 acgcggagcg ccgcatctac ccggtgctag gtgaagtggc ggtcacagag atgacgccag
     5041 ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca cccgactgcc cgccggcatg
     5101 cctacaacgt cctccgggcg gtgatgaaca cagcggtcga ggacaagctg atcgcagaga
     5161 acccgtgccg gatcgagcag aaggcagccg atgagcgcga cgtagaggcg ctgacgcctg
     5221 aggagctgga catcgtcgcc gctgagatct tcgagcacta ccggatcgcg gcatacatcc
     5281 tggcgtggac gagcctccgg ttcggagagc tgatcgagct tcgccgcaag gacatcgtgg
     5341 acgacggcat gacgatgaag ctccgggtgc gccgtggcgc ttcccgcgtg gggaacaaga
     5401 tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc tgtgacggtt ccgcctcacg
     5461 tcgcggagat gatccgagcg cacatgaagg accgtacgaa gatgaacaag ggccccgagg
     5521 cattcctggt gaccacgacg cagggcaacc ggctgtcgaa gtccgcgttc accaagtcgc
     5581 tgaagcgtgg ctacgccaag atcggtcggc cggaactccg catccacgac ctccgcgctg
     5641 tcggcgctac gttcgccgct caggcaggtg cgacgaccaa ggagctgatg gcccgtctcg
     5701 gtcacacgac tcctaggatg gcgatgaagt accagatggc gtctgaggcc cgcgacgagg
     5761 ctatcgctga ggcgatgtcc aagctggcca agacctcctg aaacgcaaaa agcccccctc
     5821 ccaaggacac tgagtcctaa agaggggggt ttcttgtcag tacgcgaaga accacgcctg
     5881 gccgcgagcg ccagcaccgc cgctctgtgc ggagacctgg gcaccagccc cgccgccgcc
     5941 aggagcattg ccgttcccgc cagaaatcta gacggtgacc acaacgcgcc cgctttgatc
     6001 ggggacgtcg a
//
