LOCUS       sequence-332830-        9870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression, SpyCas9 prime editor with 3HA-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Liu P, Liang SQ, Zheng C, Mintzer E, Zhao YG, Ponnienselvan K, Mir 
            A, Sontheimer EJ, Gao G, Flotte TR, Wolfe SA, Xue W
  TITLE     Improved prime editors enable pathogenic allele correction and 
            cancer modelling in adult mice.
  JOURNAL   Nat Commun. 2021 Apr 9;12(1):2121. doi: 10.1038/s41467-021-22295-w.
  PUBMED    33837189
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-04-9"; volume: "12(1):2121. doi"; pages: " 
            10.1038/s41467-021-22295-w"
COMMENT     Sequence Label: SpyCas9 PE2-3HA
FEATURES             Location/Qualifiers
     source          1..9870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             314..334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(335..2848,2849..2851,2852..4435)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             335..4435
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6605..6694
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             6725..6745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6754..6771
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6794..6811)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6800..7024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7095..7111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7095..7111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7108..7130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7119..7135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7143..7149,7150..7167,7168..7173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7188..7209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7326..7343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7497..8085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7577..7596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8256..9047,9048..9116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8879..8898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9117..9221)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9300..9319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9491..9870
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aaacggacag ccgacggaag
      301 cgagttcgag tcaccaaaga agaagcggaa agtcgacaag aagtacagca tcggcctgga
      361 catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa
      421 gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc
      481 cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag
      541 aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat
      601 ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga
      661 taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga
      721 gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga
      781 cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat
      841 cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca
      901 gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc
      961 catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc
     1021 cggcgagaag aagaatggcc tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc
     1081 caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac
     1141 ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt
     1201 tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac
     1261 cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca
     1321 ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat
     1381 tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga
     1441 agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct
     1501 cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat
     1561 cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta
     1621 cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta
     1681 ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga
     1741 ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag
     1801 cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa
     1861 gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt
     1921 gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga
     1981 cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa
     2041 gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc
     2101 cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga
     2161 ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga
     2221 gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca
     2281 gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat
     2341 ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa
     2401 cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa
     2461 agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag
     2521 ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt
     2581 gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac
     2641 ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga
     2701 gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa
     2761 gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat
     2821 caaccggctg tccgactacg atgtggacgc tatcgtgcct cagagctttc tgaaggacga
     2881 ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt
     2941 gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa
     3001 gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga
     3061 actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca
     3121 cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat
     3181 ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt
     3241 ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa
     3301 cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta
     3361 cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg
     3421 caaggctacc gccaagtact tcttctacag caacatcatg aactttttca agaccgagat
     3481 taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg
     3541 ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc
     3601 ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat
     3661 cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa
     3721 gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga
     3781 aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga
     3841 aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt
     3901 gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg
     3961 gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc
     4021 caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga
     4081 ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat
     4141 cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt
     4201 gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat
     4261 ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac
     4321 catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca
     4381 gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag gtgactctgg
     4441 aggatctagc ggaggatcct ctggcagcga gacaccagga acaagcgagt cagcaacacc
     4501 agagagcagt ggcggcagca gcggcggcag cagcacccta aatatagaag atgagtatcg
     4561 gctacatgag acctcaaaag agccagatgt ttctctaggg tccacatggc tgtctgattt
     4621 tcctcaggcc tgggcggaaa ccgggggcat gggactggca gttcgccaag ctcctctgat
     4681 catacctctg aaagcaacct ctacccccgt gtccataaaa caatacccca tgtcacaaga
     4741 agccagactg gggatcaagc cccacataca gagactgttg gaccagggaa tactggtacc
     4801 ctgccagtcc ccctggaaca cgcccctgct acccgttaag aaaccaggga ctaatgatta
     4861 taggcctgtc caggatctga gagaagtcaa caagcgggtg gaagacatcc accccaccgt
     4921 gcccaaccct tacaacctct tgagcgggct cccaccgtcc caccagtggt acactgtgct
     4981 tgatttaaag gatgcctttt tctgcctgag actccacccc accagtcagc ctctcttcgc
     5041 ctttgagtgg agagatccag agatgggaat ctcaggacaa ttgacctgga ccagactccc
     5101 acagggtttc aaaaacagtc ccaccctgtt taatgaggca ctgcacagag acctagcaga
     5161 cttccggatc cagcacccag acttgatcct gctacagtac gtggatgact tactgctggc
     5221 cgccacttct gagctagact gccaacaagg tactcgggcc ctgttacaaa ccctagggaa
     5281 cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc cagaaacagg tcaagtatct
     5341 ggggtatctt ctaaaagagg gtcagagatg gctgactgag gccagaaaag agactgtgat
     5401 ggggcagcct actccgaaga cccctcgaca actaagggag ttcctaggga aggcaggctt
     5461 ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc cccctgtacc ctctcaccaa
     5521 accggggact ctgtttaatt ggggcccaga ccaacaaaag gcctatcaag aaatcaagca
     5581 agctcttcta actgccccag ccctggggtt gccagatttg actaagccct ttgaactctt
     5641 tgtcgacgag aagcagggct acgccaaagg tgtcctaacg caaaaactgg gaccttggcg
     5701 tcggccggtg gcctacctgt ccaaaaagct agacccagta gcagctgggt ggcccccttg
     5761 cctacggatg gtagcagcca ttgccgtact gacaaaggat gcaggcaagc taaccatggg
     5821 acagccacta gtcattctgg ccccccatgc agtagaggca ctagtcaaac aaccccccga
     5881 ccgctggctt tccaacgccc ggatgactca ctatcaggcc ttgcttttgg acacggaccg
     5941 ggtccagttc ggaccggtgg tagccctgaa cccggctacg ctgctcccac tgcctgagga
     6001 agggctgcaa cacaactgcc ttgatatcct ggccgaagcc cacggaaccc gacccgacct
     6061 aacggaccag ccgctcccag acgccgacca cacctggtac acggatggaa gcagtctctt
     6121 acaagaggga cagcgtaagg cgggagctgc ggtgaccacc gagaccgagg taatctgggc
     6181 taaagccctg ccagccggga catccgctca gcgggctgaa ctgatagcac tcacccaggc
     6241 cctaaagatg gcagaaggta agaagctaaa tgtttatact gatagccgtt atgcttttgc
     6301 tactgcccat atccatggag aaatatacag aaggcgtggg tggctcacat cagaaggcaa
     6361 agagatcaaa aataaagacg agatcttggc cctactaaaa gccctctttc tgcccaaaag
     6421 acttagcata atccattgtc caggacatca aaagggacac agcgccgagg ctagaggcaa
     6481 ccggatggct gaccaagcgg cccgaaaggc agccatcaca gagactccag acacctctac
     6541 cctcctcata gaaaattcat caccctctgg cggctcaaaa agaaccgccg acggcagcga
     6601 attctaccca tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc
     6661 aggatcgtat ccttatgacg ttccagatta cgctaaaaga accgccgacg gcagcgaatt
     6721 cgagcccaag aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa
     6781 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6841 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     6901 aaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     6961 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     7021 atggcttctg aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc
     7081 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7141 cacaacatac gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa
     7201 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7261 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7321 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7381 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7441 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7501 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7561 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7621 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7681 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7741 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7801 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7861 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7921 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7981 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8041 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8101 ttttctacgg ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8161 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8221 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8281 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8341 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8401 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8461 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8521 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8581 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8641 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8701 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8761 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8821 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8881 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8941 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9001 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9061 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9121 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9181 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9241 ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc tactctcagt
     9301 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     9361 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     9421 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     9481 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     9541 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     9601 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     9661 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     9721 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     9781 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     9841 atctacgtat tagtcatcgc tattaccatg
//
